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We constructed a CALB-displaying yeast whole-cell biocatalyst and applied it to esterification from caproic acid and ethanol.

重组酵母菌经冻干能有效地实现在非水相中全细胞催化己酸和乙醇酯化合成己酸乙酯。

The main esters in Chinese distilled liquor include,ethyl caproate,ethyl acetate,ethyl lactate and ethyl butyrate etc.

中国蒸馏酒中含有一定比例的乙酸乙酯、乳酸乙酯、己酸乙酯和丁酸乙酯等,这几种酯的含量在一定程度上形成了中国蒸馏酒与外国蒸馏酒很不相同的地方。

In order to improve interlayer pit mud fermentation, self-made pit mud fermenter was used and pit mud functional bacteria were supplied in each turn of fermentation. Such measures could evidently increase contact area between fermented grains and pit mud, enhance ethyl caproate content in fermented grains (enhanced by almost three times than before) with no adverse influence on liquor yield, and improve liquor quality. Its application could effectively settle the problems such as pit mud odor in liquor, difficult operation, recycle use and unsatisfactory effects etc.

采用自制夹泥发酵器和每轮补充窖泥功能菌的夹泥发酵法进行浓香型白酒生产,结果表明,该方法能明显增大糟醅接触窖泥的面积;提高糟醅中己酸乙酯等香味物质的含量,己酸乙酯的含量比对照糟醅增加了近3倍;不影响曲酒的出酒率;可明显提高曲酒的质量;有效地解决了窖泥易进入糟醅,使酒中出现明显的窖泥味、操作困难、难以循环使用、效果不明显等问题。

The trial use of M-6 in Xifeng Luzhou-flavor liquor had achieved satisfactory effects compared with contrast samples: evident increment of ethyl caproate content, pure and mellow liquor flavor, enjoyable liquor aftertaste and clean liquor body, and better liquor quality.

在酿酒生产试用中,加红曲霉M-6生产的麸曲发酵酒比对照酒己酸乙酯高2倍多;在西凤浓香酒生产中试用效果比较明显,产酒己酸乙酯增高,酒窖香纯正浓郁、酒体醇厚爽净、回味好,质量优于对照样。

And the characteristics of body their trace components are as follows: Luzhou-flavour liquor has ethyl caproate the main flavour component with its content 160~250mg/100ml (above 40% of total ester); Fen-flavour liquor has ethyl acetate the main body flavour component, above 50% of total ester, and the content of ethyl lactate and ethyl acetate above 90% of total ester; Tte main body flavour component of Maotai-flavour liquor is still uncertain, and it has higher furfural content (260mg/100ml); Sanhua-flavour liquor,β-phenethyl alcohol is a special characteristic for it and the content of ethyl lactate is 73% of total ester; In other flavour liquors,the specific flavouring components vary because of different types.

其微量成分特征为:浓香型白酒以己酸乙酯为主,含量在160~250mg/100ml,占总酯40%以上;清香型白酒以乙酸乙酯为主体香,占总酯的50%以上,乙酸乙酯与乳酸乙酯的含量占总酯90%以上;酱香型白酒的主体香暂无定论,其糠醛含量较高,达260mg/100ml;米香型白酒以β-苯乙醇为特征成分,乳酸乙酯含量占总酯高达73%。其他香型白酒因门类不同,以数种成分为其特征。

Lipase mediates esterification between caproic acid (0.6mol/L) and ethanol (0.6mol/L) in n-heptane at 40℃. The decrease in caproic acid is measured by titration and 1 unit of lipase synthetic activity is defined as 1 μmol caproic acid consumed per min.

在40℃下,脂肪酶催化己酸(0.6mol/L)与乙醇(0.6mol/L)在正庚烷中进行酯合成反应,通过滴定法测定剩余的己酸,1个脂肪酶有机相合成酶活单位定义为:在测定条件下每分钟消耗1μmol己酸的酶量。

Isocyanurate based on TDI was synthesized and the heat-resistance property of casting polyurethane elastomer which synthesized using isocyanurate based on TDI and PEPA was obviously enhanced compared to polyurethane elastomer which did not introduce the isocyanurate by the TGA under the different temperature and the mechanics performance maintenance rate at high temperature.

在催化剂作用下,合成了TDI-100型异氰脲酸酯,并与己二酸乙二醇丙二醇酯合成了浇注型聚氨酯弹性体,通过不同温度下的热失重分析和弹性体的力学性能高温保持率对比分析表明,在TDI型聚氨酯弹性体中引入异氰脲酸酯基团,可明显提高弹性体的耐热性能。

A sort of lipase producing strain with relatively high esterification rate of ethyl hexanoate was obtained was isolated from Lavandula vera L. root soil. The strain was identified and named as Arthrobacter Y-605. The optimal lipase producing conditions of the strain was indicated in details and the results were as follows:2% maize meal, 1% soy flours, 0.1% urea, 0.05% MgSO4, 0.1% K2HPO4, 0.15% KCl.

以有机相中合成酶活为指标,通过对薰衣草根际土壤产脂肪酶菌株的筛选,获得了一株在有机相中合成己酸乙酯转化率相对较高的细菌Arthrobacter Y-605,其己酸乙酯转化率为67.53%。

A series of 3 - aryl - 3 -( 5,5 - dimethyl - 3 - hydroxy - 2 - cyclohexene - 1 - one - 2 - yl) propanoic ester s were synthesized by reaction between aromatic aldehydes ,5,5- dimethyl -1,3-cyclohexanedione and isopropylidene malonate catalyzed by KF - Al2O3 in alcohols.

在KF-Al_2O_3催化下,将芳香醛3,5-二甲基-1,3-环己二酮及丙二酸亚异丙酯在醇中反应,一锅式合成了一系列的3-芳基-3-(5,5-二甲基-3-羟基-2-环己烯-1-酮-2-基)丙酸酯。

The esterification reaction and itsinfluencing factors of hexanoic acid with n-butanol, catalyzed by lipaseNovozym100T, were studied in water-in-oil microemulsions formed by alkylpolyglucoside C_(12)G_(1.50)/n-butanol/cyclohexane/water system.

研究发现,十二烷基聚葡糖苷C_(12G_(1.50)/正丁醇/环己烷/H_2O体系形成的油包水微乳液中,脂肪酶Novozym100T催化正己酸和正丁醇的酯合成反应,反应初始速率V_0随正己酸的初始浓度C_~0增大而增大;随正丁醇的初始浓度C_~0的增大而减小,后V_0进入一&坪区&。

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