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We constructed a CALB-displaying yeast whole-cell biocatalyst and applied it to esterification from caproic acid and ethanol.

重组酵母菌经冻干能有效地实现在非水相中全细胞催化己酸和乙醇酯化合成己酸乙酯。

In order to research the relations of carbon sources to structure of PHAs, six kinds of PHAs sample were synthesized by activated sludge with six different carbon sources respectively in the aerobic-anaerobic-anoxic process. Their structures were determined by 〓HNMR、〓CNMR and GC-MS. When the carbon source was acetate, the unit composition of PHAs was 93. 91mol% 3-hydroxybutanoic acid and 6. 09mol% 3-hydroxypentanoic acid ; When the carbon source was propionate, the unit composition of PHAs was 28. 66mol% HB, 63. 13mol%HV, 2. 55mol% 2-methy-3-hydroxy-butanoic acid (2MHB) and 5. 66mol% 2-methyl-3-hydroxypentanoic acid (2MHV); When the carbon source was 80% butyrate and 20% 1, 4-butanediol, the unit composition of PHAs was 65. 03mol% HB, 28. 06mol%HV, 1. 91mol%2MHB, 2. 69mol% 2MHV, 0.73mol% 4-hydroxy butanoic acid (4HB), 0.78mol% 4-methyl-3-hydroxy-pentanoic acid (4MHV), 0.80mol% 3-hydroxyhexanoic acid ; When the carbon source was 20% butyrate and 80% 1, 4-butanediol, the unit composition of PHAs was 61. 39mol% HB, 23. 01mol%HV, 4. 58mol%2MHB, 5. 97mol% 2MHV, 0.91mol% 4HB, 2. 37mol% 4MHV, 1. 77mol% HHx; When the carbon source was 60% pentanoate and 40%glucose, the unit composition of PHAs was 41. 24mol% HB, 58. 76mol%HV; When the carbon source was 60% benzoate and 40% glucose, the unit composition of PHAs was 65. 48mol% HB and 34. 52mol% HV.

为了研究不同碳源与产物PHAs结构的关系,采用好氧-厌氧-缺氧模式利用6种不同碳源培养活性污泥得到6种不同的PHAs样品,通过〓HNMR、〓CNMR和GC-MS谱图确定了这6种PHAs的单体成分和比例:以乙酸钠培养活性污泥得到的PHAs的单体组成是93.91mol%3-羟基丁酸和6.09mol%3-羟基戊酸;以丙酸钠培养活性污泥得到的PHAs的单体组成除28.66mol%HB、63.13mol%HV外,另外还有2.55mol%3-羟基2-甲基丁酸(2MHB)和5.66mol%3-羟基2-甲基戊酸(2MHV);以80%丁酸钠和20%1,4-丁二醇混合溶液培养活性污泥得到的PHAs含有七种单体组成:65.03mol%HB,28.06mol%HV,1.91mol%2MHB,2.69mol%2MHV,0.73mol%4-HB(4-羟基丁酸),0.78mol%4MHV(3-羟基-4-甲基戊酸),0.80%HHx(3-羟基己酸);以20%丁酸钠和80%1,4-丁二醇培养活性污泥所得到的PHAs含有与3#样品相同的七种单体,只是比例不同,分别是61.39mol%,23.01mol%,4.58mol%,5.97mol%,0.91mol%,2.37mol%,1.77mol%;以60%戊酸钠和40%葡萄糖培养活性污泥所得到的PHAs由HB和HV组成,比例是41.24 mol%∶58.76 mol%;以60%苯甲酸钠和40%葡萄糖培养活性污泥所得到的PHAs也由HB和HV组成,比例是65.48 mol%∶34.52 mol%。

In order to improve interlayer pit mud fermentation, self-made pit mud fermenter was used and pit mud functional bacteria were supplied in each turn of fermentation. Such measures could evidently increase contact area between fermented grains and pit mud, enhance ethyl caproate content in fermented grains (enhanced by almost three times than before) with no adverse influence on liquor yield, and improve liquor quality. Its application could effectively settle the problems such as pit mud odor in liquor, difficult operation, recycle use and unsatisfactory effects etc.

采用自制夹泥发酵器和每轮补充窖泥功能菌的夹泥发酵法进行浓香型白酒生产,结果表明,该方法能明显增大糟醅接触窖泥的面积;提高糟醅中己酸乙酯等香味物质的含量,己酸乙酯的含量比对照糟醅增加了近3倍;不影响曲酒的出酒率;可明显提高曲酒的质量;有效地解决了窖泥易进入糟醅,使酒中出现明显的窖泥味、操作困难、难以循环使用、效果不明显等问题。

The trial use of M-6 in Xifeng Luzhou-flavor liquor had achieved satisfactory effects compared with contrast samples: evident increment of ethyl caproate content, pure and mellow liquor flavor, enjoyable liquor aftertaste and clean liquor body, and better liquor quality.

在酿酒生产试用中,加红曲霉M-6生产的麸曲发酵酒比对照酒己酸乙酯高2倍多;在西凤浓香酒生产中试用效果比较明显,产酒己酸乙酯增高,酒窖香纯正浓郁、酒体醇厚爽净、回味好,质量优于对照样。

The text tells the relations of caproate bacteria and pit mud, methane bacteria ,pit mud and Fen-flavour liquor,Luzhou-flavour liquor,Feng-flavour liquor,analyses the aging reason and prevention measures of pit mud,and introduce the prescripionmaterials chooseoperating and manage of manmade pit mud.

己酸菌与窖泥、己酸菌与甲烷菌、窖泥与清香、浓香、凤香等白酒之间的关系作了详细阐述,分析了浓香型白酒生产中窖泥退化的原因及防治措施,并就人工窖泥培养的配方、原料选择、工艺操作及管理等方面作了介绍。

By using sodium bisulfate as the catalyst, hexyl hexanoate was synthesized.

采用硫酸氢钠为催化剂合成了己酸己酯,考察了催化剂用量、醇酸物质的量比、反应时间、带水剂用量及催化剂重复使用次数等因素对收率的影响。

Methods and Results— We conducted a meta-analysis to compare aprotinin,-aminocaproic acid, and tranexamic acid with placebo and head to head on 8 clinical outcomes from 138 trials.

方法和结果:我们对138个试验中的8项临床数据进行了一项荟萃分析来比较蛋白酶抑制剂、-氨基己酸、氨甲环酸(反式对氨甲基环己烷羧酸)和安慰剂的作用。

Nonaqueous titration;Aminocaproic acid;Hydrochloride;Hyperehloric acid-acetic acid;Potentiometric titration

非水滴定;6-氨基己酸;6-氨基己酸盐酸盐;冰乙酸-高氯酸;电位滴定

It was increased ethyl hexanoate synthesis substrate concentration mediated by Rhizopus chinensis 2113 whole-cell lipase after improved synthetic activity and optimized reaction condition. The reaction condition were: caproic acid concentration 2.4mol/L, substrate molar concentration ratio 1:1.1, initial water content ≤0.2%, lipase water activity 0.3~0.66, enzyme amount 16%, reaction temperature 30℃, agitation speed 150 r/min.

通过合成酶活的提高及反应条件的优化,提高了华根霉2113全细胞脂肪酶催化己酸乙酯合成的反应底物浓度,其反应条件为:己酸浓度为2.4mol/L;酸醇摩尔浓度比1:1.1;反应体系含水量≤0.2%;脂肪酶水活度0.3~0.66;加酶量16%;反应温度30℃;150r/min振荡反应。

The esterification reaction and itsinfluencing factors of hexanoic acid with n-butanol, catalyzed by lipaseNovozym100T, were studied in water-in-oil microemulsions formed by alkylpolyglucoside C_(12)G_(1.50)/n-butanol/cyclohexane/water system.

研究发现,十二烷基聚葡糖苷C_(12G_(1.50)/正丁醇/环己烷/H_2O体系形成的油包水微乳液中,脂肪酶Novozym100T催化正己酸和正丁醇的酯合成反应,反应初始速率V_0随正己酸的初始浓度C_~0增大而增大;随正丁醇的初始浓度C_~0的增大而减小,后V_0进入一&坪区&。

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