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However, relatively lower column utilization limits its application to the complex proteome isolation. In case of binding biotin molecules to a specific kind of functional groups existed in protein structure, and flowing the digested peptides over the affinity column in which avidin has been immobilized, biotinylated peptides can be selectively separated and the complicated system is simplified.

因此根据需要,变换生物素试剂与蛋白质反应的作用位点,就可以选择性地分离出不同的目标肽段,从而大大提高亲和色谱柱的使用效率,降低亲和分离的成本;结合乙酰化同位素标记技术,还可以建立多种针对不同目标肽段的特色定量分析方法,ICAT即是将同位素标记与蛋白质巯基生物素化亲和分离成功结合的一个范例。

So it is suitable for the conventional DNA extraction of cotton Verticillium wilt pathogen.

结果表明:CTAB法比氯化苄法提取的DNA片段长(50kb),纯度高(OD260/OD280=1.56),且稳定、无污染,适合于常规分子生物学研究;该提取方法中EDTA,NaCl和β-巯基乙醇的最佳配比为10 mmol,1.4 mol和2%。

Where sterility test media are to be used in the Direct Inoculation of the Culture Medium method under Test for Sterility of the Product to be Examined , modify the preparation of Fluid Thioglycollate Medium and the Soybean–Casein Digest Medium as follows.

当无菌检查培养基用于供试产品无菌检查项下的培养基直接接种法时,按如下内容变更巯基醋酸盐液体培养基和大豆-酪蛋白消化物培养基的制备方法。

In addition, two control systems of which control system Ⅰand Ⅱ were, respectively, 2-mercaptobenzimidazole and 〓Br〓 stabilized silver colloids were prepared and studied.

另外,制备了两个对照体系Ⅰ和Ⅱ,分别为2-巯基苯并噻唑和〓Br〓稳定的银胶体,并对其进行了研究。

Using poly(benzyl-ether) dendrons with thiol functionalities and CNTs/CdS core-shell nanowires as reactants, using chloroform as solvent, through adjusting the temperature and time, soluble CNTs/CdS core-shell nanowires have been obtained.

采用带巯基的聚苯甲醚树枝状分子和CNTs/CdS核壳纳米线在有机溶剂中作用,通过调节反应时间和温度,成功制备了可溶性CNTs/CdS核壳纳米线结构。

After γ ray treatment, two kinds of cells were incubated on 6-well gelatin-coated plate with density of 2.5×104/cm3. hESCs were cultured on HAFi or MEF feeder cells containing β-mercaptoethanol DMEM/F12 and basic fibroblast growth factor.

取人胚胎干细胞,分别接种在小鼠胚胎成纤维细胞或永生化人成纤维细胞饲养层上,加入含β-巯基乙醇的DMEM/F12培养基,使用前添加碱性成纤维细胞生长因子。

Results of the FTIR spectra indicate that the mercaptosuccinic acid chains are assembled on the gold cores via the Au-S bond.

傅里叶变换红外光谱仪结果表明,巯基丁二酸与金形成了Au—S键,并得到了Auxy形式的MPC。

These derivatives are readily obtained by alkylation of 2-mercaptopyridine and alkyl halides under alkaline conditions.

这些衍生物可以容易地在碱性条件下通过2-巯基吡啶和卤代烷的烷基化而得到。

We also find that the bioactivitiesincrease with the decrease of the saturation of the linkers.

本文还用 2,5-二巯基-1,3,4-噻二唑与卤代物反应合成了六种噻二唑衍生物。

Tissue concentrations of Cys is maintained at low levels by regulated production and efficient removal of these thiols.

组织中Cys的浓度通过调节Cys生成和有效移除巯基维持在较低水平。

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Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。