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When proposed method was used to determine Hgin water by Separating and Enriching Sulphydryl Cotton ,satisfactory results were obtained,the relative st.

新建方法联结巯基棉分离富集汞,用于环境水样中痕量汞的测定,相对标准偏差为2.1%~4.7%,样品加标回收率为99.3%~101.2%,结果满意。

Both different sodiumalginate and CaCl_2 concentration had significant influences on active sulphydryl;CaCl_2 had a significant influence on shear force and pH, Ca~(2+) can tender the meatdirectly or indirectly, and affect the pH of meats.

不同的海藻酸钠浓度和氯化钙浓度对于活性巯基的含量有显著的影响;不同的氯化钙浓度对于剪切力和pH也有显著的影响。

Through analyzing of the theory and the results, we concluded that method 2, namely mixed reaction of defatted soy flour, Urea-SDS solution and Ellman's reagent, can determined exactly sulphydryl content of defatted soy flour.

通过理论和数据分析得到,方法二即脱脂豆粕、尿素-SDS溶液和Ellman's试剂相混合反应可用于测定脱脂豆粕中的巯基含量。

The dry heated defatted soy flour was treated with three different methods respectively. then the sulphydryl content of defatted soy flour was determinated with Ellman's reagent.

采用3种不同方法对干热处理后的脱脂豆粕进行处理,再加入Ellman's试剂来测定脱脂豆粕中的巯基含量。

The hardness of the mixed gels increased with salts concentration, while the protein solubility, water-holding capacity and sulphydryl group content had a decreased trend. The microstructure of mixed gels showed a porous network and particulates structures by addition of NaCl and CaCl2 respectively.

结果表明:随着pH的增加,凝胶的硬度和溶解度减小,添加淀粉进一步降低了混合凝胶的溶解度;盐浓度的增加使混合凝胶硬度增加,而蛋白溶解度、持水力和巯基含量则呈下降趋势;添加NaCl 和CaCl2的混合凝胶分别呈现出多孔网状和颗粒状结构。

The key point for preparing the isolated 1D metal driven nanowires is the non-stoichemetric additions of metal ions and thiolic ligands.

其中,金属离子与巯基乙酸的非等量反应是分离状态螺旋结构的纳米带形成的主要原因。

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2~3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12,在酵母细胞中实现了多拷贝强表达;荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液,测定510 nm处的荧光发射强度结果显示,表达的绿色荧光蛋白对氧化还原水平敏感,且在510 nm处的荧光强度与一定的重金属浓度呈正相关,即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强,从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用;同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属;遗传学的点突变频率及致死率实验数据表明,重金属能导致菌体的点突变频率和致死率升高,且活性氧的清除剂巯基脲能明显降低这种点突变和致死率,说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

The morning venous blood was collected to determine the serum level of glyceryl trinitrate, cholesterol and the plasma level of Ox-LDL, malondyladehyde and superoxide dismutase before administration and 8, 16 weeks after.

本研究旨在探讨高血压病患者是否存在血浆Ox-LDL增高现象,口服化学结构无巯基的血管紧张素转换酶抑制剂降压的同时,是否会对Ox-LDL产生影响。

As a result of the analysis of unreduced SDS-PAGE, the content of large molecular rCPB polymers was decreased inβ-ME solutions as well.

经非还原SDS-PAGE分析,添加β-巯基乙醇后,溶解rCPB聚体的含量减少。

And probable mechanism for the solubility differences was illuminated by the analysis of unreduced SDS-PAGE. Results The purity and following treatment of inclusion bodies of CPB were influenced by the culturing temperature after the inducement with IPTG.

结果诱导后生长温度不同对rCPB包涵体的纯度及后期处理均有影响;rCPB包涵体在10 mol/L尿素溶液中的溶解度比在8 mol/L 尿素溶液中提高2~3倍;添加0.75%β-巯基乙醇能显著改善rCPB包涵体的溶解效果。

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