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The eyes were periodically examined by retinoscopy, and A-scan ultrasonography. Two weeks later, the eyeballs were removed and the apoptotic cells were determined by electron microscopy, TUNEL technique and flow cytometry in scleras. Both bFGF and the bcl-2 protein were determined by the immunohistochemisty method and the Western-blot method. Results: All eyes treated with -10.00D or -20.00D concave lens produced defocus-induced myopia.

实验前后用睫状肌麻痹下验光、A超动态观察豚鼠眼屈光度和眼轴长度,并取后极部巩膜组织,用电镜、终末脱氧核糖核苷酸转移酶介导原位缺口末端标记法和流式细胞术检测巩膜的凋亡细胞,应用免疫组织化学染色和蛋白印迹(Western-blot)法检测bFGF和bcl-2蛋白。

Hematoxylin and eosin staining, and Masson staining were used to study the pathological changes of sclera.

应用苏木精伊红染色和Masson染色对照观察激光和刀片切口术后巩膜的病理变化。

It snggests that the isotropy assumption could be used for preliminary mechanical model of cornea.

众多学者已意图研究角膜和巩膜的非线性模量和蠕变反应等生物力学特性,其中值得

Elevation of IOP changes the curvature of the sclera but not that of the cornea.

IOP的升高改变了巩膜的曲率而不改变角膜的。

The irritated sclera was observed by light microscopy and scanning electron microscopy.

应用光学显微镜和扫描电镜观察激光作用后巩膜的形态学变化,并用Nd:YAG激光作为对比。

The elastic moduli of the cornea and sclera are independent of IOP.

结果显示,角膜和巩膜的弹性系数不受IOP的影响。

Results NGF and its mRNA were localized in chondrocytes in the sclera, especially in the binucleate cell. In NGF and its mRNA respectively, the amount of the positive cells in the occluded eyes was significantly higher than that of the control eyes (P.05). In NGF F(subscript DG=20.556, P.001 or in its mRNA F(subscript DG=25.520, P.001, the amount of the positive cells among the occluded groups or the control groups was significantly decreased along with the time.

结果 NGF蛋白及其mRNA均表达於巩膜的软骨细胞中尤其在双核细胞中;两者在各时间段遮盖眼巩膜中阳性细胞数均明显高於对照组(P.05);免疫组织化学F(下标 DG=20.556 P.01和原位杂交F(下标 DG=25.520 P.01在对照组各组间阳性细胞的表达数目随时间变化明显下降。

AIM: Growth factors at certain concentration in body are essential for the normal development of eyeball; either less or more can result in scleral abnormity.

目的:研究表明,体内一定浓度的生长因子是保证眼球正常发育的必要条件,过少或过量都会引起巩膜的异常改变。

AbstractAIM: To study the changes of refractive status and axial length in chicken form deprivation myopia and its recovery model, to observe the metamorphism of posterior sclera and to lay foundation for investigation of axial myopia pathogenesisMETHODS: Twenty-five newly incubated chicken were selected. Their left eyes were covered with semi-translucent membrane to deprive form perception for 14 days (form-deprivation group), or covered for 11 days followed by uncovered for 3 days. Unoccluded eyes in either group served as control eyes respectively. Retinoscopy and A- ultrasound were conducted to collect the refraction and axial length data.

目的:研究雏鸡形觉剥夺性近视眼及形觉剥夺性近视眼恢复模型的屈光状态和眼轴长度的变化,并观察后极部巩膜的形态学改变,为探讨轴性近视的发病机制打下基矗方法:选用新孵出的普通肉食家鸡25只,采用半透明薄膜眼罩遮盖的方法对左眼进行形觉剥夺14d或遮盖11d后,去遮盖3d。

The experimental method was improved, by using a ball to press the sclera samples, which were obtained nine months after surgery. The pretesting, relaxing and creeping experiments were carried out.

改进实验方法,采用球面致压拉伸巩膜的实验方法,对术后9月组上述5种兔眼巩膜试件进行预拉伸、应力松弛、蠕变的生物力学实验研究。

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