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Cauterizing the episcleral vessels to build chronic elevated IOP can result in the decrease of stained grains in RGCs nucleus.

1。采用Akira法,烙闭大鼠右眼上巩膜静脉,制作大鼠持续性高眼压模型可导致RGCs细胞核内银染颗粒减少。

The models of EIOP were established by obstructing episcleral veins, using immunohistochemistry stain for PEDF expression in retina and evaluate the rule of the change.

应用巩膜浅层静脉烧烙法制作慢性高眼压(elevated intraocular pressure, EIOP)模型,用免疫组织化学染色法观察视网膜各层结构PEDF的表达和变化规律。

A diode laser with wavelength of 532-nm was used to burn the trabecular meshwork and episcleral veins of right eyes at 45 SD rats, while the left eyes were referred as control.

SD大鼠45只,麻醉后使用532二极管激光行右眼角膜缘360°光凝,角膜缘激光斑为80~100个,大鼠角膜缘颞侧、颞上及颞下巩膜浅层静脉3条,每条静脉光凝3~4个斑点,功率

Methods Condensation of the enternal sclera,episcleral buckling,cerculer ligation and injection of air to the vitreous body were performed in 36 cases with Ⅲ-grade retinal detachment.

选择本院Ⅲ级膜视网膜脱离 36例,应用巩膜外冷凝、垫压、环扎、压力空气作分离膜牵引的操作工具,塑形充填剂于Ⅲ级膜视网膜脱离的治疗。

Methods Tweleve healthy SD rats were cauterized three episcleral vessels and were divided into two groups (tPNS treated group including normal IOP and elevated IOP, untreated group including simple normal IOP and elevated IOP)with 6 rats in each group.

健康SD大鼠12只,采用Akira法,烙闭大鼠右眼上巩膜静脉,制作大鼠持续性高眼压模型,随机分为对照组,tPNS治疗组。

IOP was raised by electrocoagulating at least 3 episcleral and limbal veins on the right eye of each rat in ocular hypertensi on group and its contralateral eye was used as control.

采用电凝鼠巩膜表面至少3组静脉及角膜缘周围血管,减少房水静脉回流升高眼压。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. The n 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule.

取健康SD大鼠60只,正常对照组和正常银杏叶治疗组各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水组,治疗A组(每日EGb 50mg/kg)、治疗B 组(每日EGb 100mg/kg)、治疗C 组(每日EGb 150mg/kg)、治疗D 组(每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

Management. Treatment of manifestations: Early diagnosis and treatment of RB and RB-related tumors can reduce morbidity and increase longevity; care is best provided by specialists from ophthalmology, pediatric ophthalmology, radiation oncology, oncology; treatment options depend on tumor stage, number of tumor foci (unifocal, unilateral multifocal, or bilateral), localization and size of the tumor within the eye, presence of vitreous seeding, and age of the child; treatment options may include enucleation, cryotherapy, photocoagulation, photochemistry, external beam radiation therapy, and radiation therapy using episcleral plaques; newer options include systemic chemotherapy combined with or followed by local therapy.

处理。 表征的治疗: RB 和 RB 相关肿瘤的早期诊断和治疗能减轻病状、延长存活时间;护理最好由眼科学、小儿眼科学、肿瘤学和放射肿瘤学等方面的专业医师来提供;治疗方案的选择取决于肿瘤阶段、病灶数量(单病灶、单侧多病灶或双侧病灶)、眼内肿瘤的位置和大小、玻璃体散布的存在与否以及患儿的年龄;治疗选项可能包括摘出术、冷冻疗法、光凝固术、光化学法、外照射放疗和用巩膜外斑块进行的放射治疗;新的治疗选项包括与局部治疗结合的或先行的系统化学疗法。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. Then 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只,正常对照组和正常银杏叶治疗组各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水组,治疗A组(每日EGb 50mg/kg)、治疗B 组(每日EGb 100mg/kg)、治疗C 组(每日EGb 150mg/kg)、治疗D 组(每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into 3 groups: 6 rats in normal control group, 6 rats in EGb treating normal IOP group, and the other 48 rats were established chronic high IOP models of rats by cauterizing two episcleral veins. The n 30 rats satisfying experimental level were selected and randomly divided into five groups: 6 rats in physiological brine treating group, and the other 24 rats were divided into four experimental groups according to the dose of EGb: group A, EGb 50mg/; group B, EGb 100mg/; group C, EGb 150mg/; and group D EGb 200mg/. After the treating time of 1 month, the rats were sacrificed on schedule. Flat preparation of whole retinaes was stained distinctively and neuron counting in retinal ganglion cell layer from both eyes of each rat were performed to evaluate neuron situation.

取健康SD大鼠60只,正常对照组和正常银杏叶治疗组各6只,其余48只采用烧烙法,烙闭大鼠左眼2条浅层巩膜静脉,制作大鼠持续性高眼压模型,从中选出眼压稳定在实验要求水平的大鼠30只,随机分为生理盐水组,治疗A组(每日EGb 50mg/kg)、治疗B 组(每日EGb 100mg/kg)、治疗C 组(每日EGb 150mg/kg)、治疗D 组(每日EGb 200mg/kg),治疗时间为1mo,处死大鼠后做视网膜全层铺片,对RGCL神经元做特异性染色后行神经元计数分析来评估神经元的情况。

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