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巨血小板

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Objective:To observe the effect of different extraction sites of peanut seed coat on mice with thrombocytopenia in the bone marrow megakaryocyte number.

目的:观察花生种皮不同提取部位对血小板减少型小鼠骨髓巨核细胞数的影响。

After therapy, we compared the change of the patients' platelet count and megakaryocyte count and the time when the bleeding symptom is disappeared.

观察治疗前及治疗开始后第3、6、9、14天血小板计数,和巨核细胞计数,并统计治疗开始后出血症状消失的时间。

Expression,distribution and function of PTA1 molecule,a novel member of immunoglobulinsuperfamily,on human megakaryocyte/platelet lineage

中文题名 PTA1在巨核细胞/血小板谱系中的表达,分布与功能研究

No significant associations of megakaryocyte quantity and anti-platelet autoantibody with the three kinds of cells were found,and there were no statistical differences between primary ITP and recurrent ITP patients.

4巨核细胞数目与Th17、Th1和Tc1细胞亚群之间无明显相关性。抗血小板抗体阳性的患者与阴性患者相比,三种细胞亚群的比例无明显差别。

Objective To explore the significant changes of transcription factor GATA- 1 and nuclear factor erygthroid 2 (NF -E2) in megakaryocytes of bone marrow in children with acute idiopathic thrombocytopenic purpura.

目的探讨转录因子GATA-1和NF-E2在急性特发性血小板减少性紫癜患儿骨髓巨核细胞表达水平的改变及其意义。

Among the cases, hypergranular promyelocytic was present in 15%. Infected bone marrow and megaloblastic anemia were observed in 5% respectively. Fungi infected bone marrow and idiopathic thrombocytopenic purpura were found in 2.5% respectively. Pure red cell aplasia and granulocytic series deficiency due to regenerative granulocyte failure were observed in 2.5% respectively.

在40例艾滋病患者中,反应性早幼粒细胞增多者占15%,感染性骨髓象和巨幼细胞性贫血各占5%,真菌感染性骨髓象和特发性血小板减少性紫癜各占2.5%,纯红再障和生成减少性粒细胞缺乏症各占2.5%。

Methods: CFU-MK and the model of thrombopenia of aplastic anemia were analysed by methylcellulose culture or with sera from patients with aplastic anemia. The number of megakaryocyte colony-forming unit and the content of CD41+ cells in CFU-MK were measured simultaneously. Results: After 14 days of culture, the number of CFU-MK and megakaryocyte cluster-forming unit of the model groups were significantly less than that of the normal control groups.

应用甲基纤维素半固体体外培养法建立CFU-MK培养体系;混合再障血清进行培养建立再障患者血小板减少的模型;使用不同的造血细胞生长因子进行组合观察模型中CFU-MK集落生成情况和集落中巨核祖细胞的含量。

At the same time, the process of the wound healing was observed histologically, and the regeneration cycle of epidermal cells and the temporal change in inflammatory cells were measured. Results Inflammatory cells infiltrated into wound surface were neutrophils, followed by macrophages and lastly lymphocytes. Epidermal cells proliferated most actively on PBD 3 and the mitoses of them increased significantly on day 7 after burn. The gene expression of PDGF, PDGFR and EGFR reached peaks on PBD 1 and the gene expression of EGF and TGFβ-R2 were highest on PBD 3. In addition, the gene expression of TGFβ-R1 and TGFβ1 increased significantly on PBDs 5 and 7 respectively.

结果 炎性细胞到达创面依次为中性粒细胞、巨噬细胞和淋巴细胞;表皮细胞周期变化为伤后3天细胞增殖活跃,伤后7天细胞分裂明显增多;伤后1,3天PDGF、PDGFR(血小板源性生长因子受体)和EGFR基因表达明显增强,伤后3,5天EGF、TGFβ-R2基因表达增强,伤后5,7天TGFβ-R1(转化生长因子β受体1)基因表达增强,伤后7,10天TGFβ1基因表达增强。

The culture supernant of transfect cells had the ability to stimulate the proliferation of HEL cell line in vitro.

注入体内了解其对巨核系造血的影响,发现大量给入时,可促进小鼠血小板增高。

TPO can inducing megakaryocyte progenitor cells differentiate committedly, and IL-3,IL-6 and TPO can synergized with TPO.

TPO可以定向诱导巨核系细胞的分化,IL-3、IL-6、SCF可以协同TPO的作用,这在造血调控研究,体外定向扩增外周血干细胞,促进外周造血干细胞移植患者血小板的恢复具有应用前景。

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