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Microparticle can carry antisense MCP-1 gene into aorta successfully, which can inhibitate MCP-1 gene expression, microphage infusion and slow down the development of AAA. At the same time, there is no MCP-1 gene expression in other position, suggesting the microparticle si an secure gene transfer carrier.

以微粒子为载体,能成功地将反义MCP-1基因导入动脉组织中,并能明显抑制内源性MCP-1基因的表达和巨噬细胞在动脉壁的浸润程度,减缓腹主动脉瘤的进一步生长,同时外源基因在机体的其他部位没有表达,提示微粒子是一种安全的基因转移载体。

Rat AAA model was made by perfusion PPE elastase through the abdominal aorta, and the specimen was obtained on postoperativeday 3, 7, 14, 28, respectively. Specific elastic fiber staining was used to observe the disruption of elastic fiber in aortic wall. Immunohistochemistry staining of CD68 was applied to observe the microphage infiltration. The in tisu hybridization and western blot of MCP-1 and MMP-2 were used to study the expression of mRNA and protein. Thus we can analyze the facilitated function of MCP-1 gene to the microphage adherence and infiltration on the earlier AAA.

采用经腹主动脉腔内导管灌注的方法制成大鼠AAA模型,分别于术后3天、1周、2周、3周、4周获取各组大鼠腹主动脉标本,行弹力纤维特殊染色观察动脉壁弹力纤维的破坏情况;行CD68免疫组织化学染色观察动脉壁中巨噬细胞的浸润;行MCP-1及MMP-2的原位分子杂交、Western蛋白质印迹观察二者的mRNA表达和蛋白表达;分析MCP-1基因促进巨噬细胞的黏附、浸润在早期AAA发病中的作用。

The conclusions are as follows:(1) As mono-macrophages and neutrophilic granulocytes increase in mumber by stimulation of LPS, it is suggested that they present a pronounced dynamic distribution.

本研究的结论是:(1)LPS可刺激脾内单核-巨噬细胞和中性粒细胞增多,表明它们有明显的动态分布;(2)脾脏NOS活性反应和NOS及NO含量有严格的时间性;(3)就幼龄大鼠来说,腹注LPS的有效毒性只能维持在24h之内。

An Australian thief got more than he bargained for when he snatched a bag containing a venomous red-bellied black snake.The bag was stolen from the boot of a professional snake catcher''s car as he stopped at traffic lights in Redfern, Sydney.

一个小偷在澳大利亚悉尼市雷德福区的一辆汽车里偷拿了一个袋子,却不料这个袋子里装着的是一个危险的动物——一条1.2米长的巨毒大腹黑蛇。

An Australian thief got more than he bargained for when he snatched a bag containing a venomous red-bellied black snake.The bag was stolen from the boot of a professional snake catchers car as he stopped at traffic lights in Redfern, Sydney.

一个小偷在澳大利亚悉尼市雷德福区的一辆汽车里偷拿了一个袋子,却不料这个袋子里装着的是一个危险的动物——一条1.2米长的巨毒大腹黑蛇。

推荐网络例句

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了,但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场,你等着挨绳子吧!

Several theories about reigniting and extinguishing of the arc have been refered.

本文综合考虑了几种电弧重燃和熄灭理论。