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Nuclear factor B DNA binding activity in alveolar macrophages and systemicmediator levels did not change.

DNA 核因子к B 结合的活跃性在肺泡中的巨噬细胞和全身的介质水平没有改变。

In their habitat researchers have found fruit pits, the fig, bitter almond and so on. They haven't seen any mark of insects, rodents and birds. Snails perhaps are the only creature eaten by the giant lizard.

研究人员在它们的栖息地发现各种水果核、无花果以及苦杏仁等,但没有任何昆虫、啮齿动物以及鸟类痕迹,蜗牛可能是这种巨蜥吃过的唯一一种动物。

Using the model of CTX mice,on"Dazhui","Shenshu"and"Zusanli"by crude herb moxibustion with cantharis anhydro preparation,examine the effect on the number of karyocyte of bone marrow and WBC and spleen index and the production ability of GM CSF by abdominal cavity macrophage.

天灸"大椎"、"肾俞"、"足三里"穴,对化疗骨髓抑制有明显的改善作用,可以显著增加骨髓有核细胞和白细胞计数,增加脾指数;对腹腔巨噬细胞产生GM CSF的能力有显著的增强作用。

Mr Qaddafi, now the world's longest-ruling dictator, with 41 years under his cartridge belt, has been re-embraced by some in the West as a reward for dropping his nuclear ambitions and because of his vast oil wealth.

作为对他放弃核野心的奖赏,同时也因为他巨量的石油财富,他又重新受到西方的拥抱。

Germinal center of various cell proliferation, nuclear fission, as seen, and there are many phagocytic cells macrophages clastic.

生发中心内各种细胞增生,核分裂像多见,并有许多吞噬了细胞碎屑的巨噬细胞。

The main pathological findings include a desquamative interstitial pneumonia-like reaction with intra-alveolar macrophages and numerous large multinucleated histiocytes that ingested inflammatory cells were admixed with macrophages.

GIP主要病理表现有脱屑性间质性肺炎样反应,即在肺泡腔内有巨噬细胞和大量的多核巨细胞聚集,位于肺泡腔内的多核巨细胞内可见被吞噬的炎性细胞,是GIP的特点。

The results indicated that the erythropoietic/granulopoietic ratio was markedly increased with megaloblastic morphology in all stage of erythrocyte.

结果表明:2例CDAⅠ型患者骨髓幼红细胞比例增加,各阶段细胞存在不同程度巨幼样变;原红细胞核不规则,早幼或中幼红细胞可见奶酪核,约半数晚幼红细胞有核溶解和破碎现象,晚幼红细胞核损伤有时伴胞质溶解,细胞间染色质桥少见;各阶段红细胞核膜和内质网同时出现不同程度溶解。

Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of MCSF (25μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed. These cells were also cultured in the presence of both RANKL (30 μg/L) and M-CSF (25 μg/L) with or without 10 μg/L TNF-α for4, 5, 6 and 9 days. The number of TRAP multinucleated cells and resorption pits on dentine slices were counted under light microscope.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞,在含有25μg/L M-CSF和0,1,10,100μg/L TNF-α的α-MEM培养液中培养5d后,观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成;细胞在含有25μg/L M-CSF和30μg/L sRANKL的α-MEM培养液中进行培养,比较加入和不加入10μg/L TNF-α培养4、5、6和9d后,所形成的TRAP多核细胞的数目和骨吸收面积。

Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of M-CSF (25 μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞,在含有25μg/LM-CSF和0,1,10,100μg/LTNF-α的α-MEM培养液中培养5d后,观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成;细胞在含有25μg/LM-CSF和30μg/LsRANKL的α-MEM培养液中进行培养,比较加入和不加入10μg/LTNF-α培养4、5、6和9d后,所形成的TRAP多核细胞的数目和骨吸收面积。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞;以不同浓度的LPS(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml)和不同作用时间(0min,5min,15min,30min,60min,120min)分别刺激小室培养的细胞单层,观察NF-κB的核内浓度及NO合成量的动态变化,选择LPS的最佳用量和作用时间;然后分成四组实验,设正常对照组,LPS处理组,特异性PKC抑制剂阻断组,NF-κB抑制剂阻断组;收集培养的单层细胞及培养液;采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平;免疫组化法检测NF-κB的核内移位变化;硝酸还原酶法测定细胞培养液中NO含量;吖啶橙染色观察凋亡细胞的形态学变化,凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

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