巨噬细胞

We got the result: Bufo Raddei Strauch Tadpole (1) Bufo Raddei Strauch Tadpole was capable of lens regeneration, which originated from the epithelial cells at iris dorsal margin by dipigmentation.(2) Dipigmentation of Bufo Raddei Strauch by two methods: one was licked up by amoebocyte, the other one was flowed from the pigment cells.(3) In the lens regeneration cells of Bufo Raddei Strauch profiles of the mitochondria became complicated ribosome manifold, and the endoplasmic reticulum was cisternal dilation. During the process of deferentiation of lens fiber the cell nucleolus became little and chromatin shrinked.(4)The crystalline electrophoresis of Bufo Raddei Strauch Tadpole lens regeneration showed that during lens regeneration P-crystalline was expressed earliest then was γ-crystalline and α-crystallin, this result is consistent with newt.

结果表明：花背蟾蜍蝌蚪(1)具有晶状体再生的能力，其晶状体的再生来源于虹膜背缘的上皮细胞；(2)虹膜背缘去色素是转分化的前提，主要有两种方式：一种也是最主要的方式是在晶状体被摘除后虹膜外侧产生巨噬细胞，巨噬细胞吞噬虹膜色素上皮细胞中的色素颗粒；另一种方式是虹膜色素上皮细胞自身释放色素颗粒；(3)电镜观察发现虹膜色素上皮细胞转分化过程中线粒体有明显变化，核糖体增多，粗面内质网增多，晶状体纤维分化过程中细胞核逐渐凝聚；(4)SDS-PAGE电泳发现，花背蟾蜍蝌蚪晶状体再生中相关蛋白表达顺序与有尾类蝾螈相似，蛋白表达顺序依次是β-晶状体蛋白、γ-晶状体蛋白和α-晶状体蛋白，并且后两种蛋白几乎同时表达。

RESULTS: Compared with control group, the activity of the LDH/ACP/Arg in the AMФ or PMФ and in the culture fluid of the AMФ or PMФ were significantly increased (P.05 or P.01), and the ability devouring neutral red and stimulating index in the PMФ were strengthened (P.01), the ergastoplasm was hyperplasia and the amount of lysosome was manifold in the spleen macrophages of old rats induced by paecilomyces cicadidae total polysaccharides.

结果：蝉拟青霉总多糖使老龄大鼠肺泡巨噬细胞、腹腔巨噬细胞胞内和细胞培养上清液内ACP、LDH、arginase活力显著高于对照组（P.05或P.01）；使PMФ的吞噬功能及其刺激指数显著高于对照组（均P.01）；使老龄大鼠脾脏细胞胞质粗面内质网和溶酶体数量多于对照组。

Results α-Linolenic acid was capable of increasing the quantity of antibody-producing cells and the level of serum hemolysin, promoting the expurgation effect and the phagocytic function of monocyte/macrophage, stimulating...

结果α-亚麻酸能提高小鼠的抗体生成细胞数和血清溶血素水平，促进小鼠的单核-巨噬细胞的碳廓清和腹腔巨噬细胞的吞噬能力，促进小鼠的迟发型变态反应，提高小鼠NK细胞的活性。结论α-亚麻酸具有增强小鼠免疫力作用。

The results shown that:(1) Bufo raddei Strauch tadpole was capable of lens regeneration, which originated from the epithelial cells at dorsal iris margin by depigmentation.(2) Depigmentation of Bufo raddei Strauch during lens regeneration was by two methods: First, pigment granules initially dispersed all over the cytoplasm were moved towards the periphery of the cell, and then were directly taken up by the amoeboid cells. Second, pigment granules in a cell were first crowded in a mass, surrounded by a membranous structure in the cytoplasm, which were eventually discharged as a mass from the cell.

结果表明：花背蟾蜍蝌蚪(1)具有晶状体再生的能力，其晶状体的再生来源于虹膜背缘的上皮细胞；(2)虹膜背缘去色素是转分化的前提，主要有两种方式：一种也是最主要的方式是在晶状体被摘除后虹膜外侧产生巨噬细胞，巨噬细胞吞噬虹膜色素上皮细胞中的色素颗粒；另一种方式是虹膜色素上皮细胞自身释放色素颗粒；(3)电镜观察发现虹膜色素上皮细胞转分化过程中线粒体有明显变化，核糖体增多，粗面内质网增多，晶状体纤维分化过程中细胞核逐渐凝聚。

Methods: Mice were inoculated with tumor cells. Inhibitory effect of transfection with pCH510 on murine tumor origined from different inoculative dose and inhibiting effect of immediate transfection pCH510 after chemotherapy on tumor were observed, respectively. By cell culture technique, the influence of chemotherapeutic drug to activation of marcrophages and lymphocytes after i.p. injection of drug was observed. By cell counting method, the kinetics of the change of number of peripheral blood immunocytes induced by administration of chemotherapeutic drug was observed. The inhibitory effect of transfection with pCH510 five days after chemotherapy on murine tumor was observed.

采用瘤细胞接种建立小鼠肿瘤模型：通过基因转染，观察pCH510对不同接种量所形成的小鼠肿瘤的抑制作用以及小鼠肿瘤化疗后立即进行pCH510转染的抑瘤效果；采用细胞培养技术，观察小鼠体内注射化疗药物后，其对小鼠腹腔巨噬细胞和脾淋巴细胞激活功能的影响；采用细胞计数的方法，观察化疗药物所致小鼠腹腔巨噬细胞和外周血免疫细胞数量变化的动力学；另外小鼠肿瘤化疗后第5天进行pCH510转染，观察抑瘤效果。

Single nucleus-macrophage function determination:mouse carbon clearance test; macrophage in the mouse peritoneal cavity phagocytose the chicken red blood cell test, with half in vivo.

小鼠碳廓清试验法测定单核-巨噬细胞功能：用半体内法进行小鼠腹腔巨噬细胞噬鸡红细胞试验。

Single nucleus-macrophage function determination; mouse carbon clearance test; macrophage in the mouse peritoneal cavity phagocytose the chicken red blood cell rest, with half in vivo.

小鼠碳廓清试验法测定单核－巨噬细胞功能：用半体内法进行小鼠腹腔巨噬细胞噬鸡红细胞试验。

However, fully consideration of special structure and physiological function of CRP is critical to investigate whether CRP provide protect effect or pathogenic effect in the progression of atherosclerosis. CRP is a member of pentrxins family, which consists of five identical, noncovalently associated 23-kDa protomers arranged symmetrically around a central pore with a 102 A external diameter. Each protomer has a recognition face with a phosphocholine binding site consisting of two coordinated calcium ions adjacent to a hydrophobic pocket. The opposite face of the pentamer is the effector face, where complement Clq binds and Fc receptors are presumed to bind. A cleft extends from the center of the protomer to the central pore of the pentamer. Both faces ofpentamer form a special bi-phase structure: one face is the ligand recognition phase, which can recognize apoptosis cells and enzyme modified low density lipoprotein in which PCh is exposed.

CRP是一种五聚体蛋白，外径为102 A，由5个相同的单体以非共价键结合，形成双面的环形结构，每个单体的配体识别位点或受体结合位点分别位于五聚体两个平面上，分别组成配体识别相和效应器相，从而构成特殊的二相性结构：一面为配体识别相，含有磷酸胆碱(phosphorycholine，PCh)结合位点，能够使CRP识别在病理条件下暴露出PCh的凋亡细胞、酶修饰低密度脂蛋白(enzyme modified low density lipoprotein，E-LDL)等。E-LDL是天然低密度脂蛋白(native Low density lipoprotein，N-LDL)在多种蛋白酶，如胰蛋白酶、神经氨酸酶、胆固醇酯酶等作用下的代谢产物，其主要特点是结构发生改变(N-LDL大小均匀，平均直径250±30 A，E-LDL大小差异极大，直径为100-2000 A)，暴露磷酸胆碱位点，能够被CRP识别；另一面为效应器相，能够与巨噬细胞、单核细胞表面受体FcγRⅡa结合，介导巨噬细胞、单核细胞识别及吞噬凋亡、坏死细胞作用。

Cells were re-suspended and cultured in a humidified incubator with 5%CO_2 at 37℃.

建立巨噬细胞和死亡细胞的共培养模型在96孔板上种植RAW264.7细胞或小鼠腹腔原代巨噬细胞，分为3组，即对照组、凋亡细胞处理组和坏死细胞处理组。

Methods Human monocyte cell line THP-1 was cultured and induced to macrophage by PMA treatment. The effect of 9-cisRA on the differentiation induced by PMA was studied. Cell morphology was observed by phase contrast microscope and the adhesion rate of THP-1 was detected by methyl thiazolyltetrazolium assay. The cell surface markers involved in differentiation of mono cyte/macmphage (CD11b, CD36) were analyzed by flow cytometry.

体外培养人单核细胞系THP-1，经佛波酯诱导分化为巨噬细胞，同时给予9-顺式维甲酸对佛波酯诱导分化进行干预，通过相差显微镜观察细胞形态，MTT比色法检测细胞贴壁率，流式细胞仪检测与单核／巨噬细胞分化有关的细胞表面标志物CD11b和CD36的表达。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。