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Results Pores were detected both on electrified erythrocytes and leukocytes with round or ellipse shapes. The erythrocytes often have one or more pores while the leukocytes often have more pores looked like cribble. The rates of perforated cells were increased with the prolonging time of electrification, 5 s with 6% and 1min increased to 40%.

结果 电击后,即可以观察到红细胞与白细胞的细胞膜穿孔,其中红细胞穿孔可为一个或多个,形状多为圆形或卵圆形,白细胞穿孔则为多个,呈筛网状;穿孔细胞数随电击时间延长而增多;电击5s时为6%左右,以后逐渐增加,至电击1min时,穿孔细胞数可达40%左右

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果:1合成的CS-PAA磁性聚合物微球呈球形,大小均一,TEM测定其干态下粒径为80nm左右,磁化曲线证实具有超顺磁性,具有一定的PH敏感性,固载表柔比星后,水溶液性状稳定,无沉淀物,DLS测定直径约200nm左右,测定载药率为15%,较文献报道高,包封率为20%。2肉眼观察试验组大鼠膀胱表面呈褐色,可见大量的Fe粒子聚集,普鲁士兰染色法显示,试验组大鼠膀胱壁内有大量的Fe粒子,分布至膀胱壁全层,与对照组大鼠相比,试验组大鼠的肝、脾内的Fe粒子聚集量明显降低;HPLC测定结果与Fe染色相同;高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高,其他组大鼠血生化指标没有明显变化。3MTT法发现,高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组,FCM发现试验药物组可引起明显的肿瘤细胞凋亡,试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C,试验组细胞Bcl-2蛋白减少,Bax蛋白变化不明显,Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P<0.01),其中高剂量组的抑制率最高。

The practice shows that the organic matter content of compositive wastewater can be reduced from about 15000 mg/L to 4000~6000 mg/L by diffluent treating saline wastewater of high contaminant concentration and dispersed treating sulfur-included wastewater, in the result the possibility of finally releasing up to par is improved.

实践表明通过对光敏剂生产废水中高含盐废水的分流处理、含硫废水分散预处理,可以使综合废水的COD从15000mg/L左右下降到4000~6000mg/L左右,提高了废水末端处理达标排放的可行性。

Mainly research results are:(1) Through electromagnetic stirring the crystal grain has been thined and the equiaxed rate enhanced from 85% to 93%.

主要研究结果为:(1)铸坯等轴晶率从未搅拌的平均85%左右提高到电磁搅拌后的平均93%左右

Supersonic wave disperse research indicated, ethanediol as the disperse medium, it could got suspending liquid about for firve days, but relatively large in grain size. However, combined use of lauryl sodium sulfate and maleic anhydride as disperse medium, the disperse result among water was better than the former, and it would be steady for two days.

超声分散研究表明,使用聚乙二醇作为分散介质,可得到分散五天左右的悬浮液,但分散后粒度较大,使用十二烷基硫酸钠和顺丁烯二酸酐混合作为分散介质在水中分散效果较好,可稳定两天左右

After Huang Qu senior official yellow Jinlong carries on the contact to finish, immediately defers to the planning for action which formulates in advance, leads side one group of terse motion members, nonstop is rapid rushes recent to the control center, nearly with the ellipse planetoid rugged surface, nearby the flatwise bend thorough giant steam hole, sees only this hole diameter to have about fully 200 meters, this time fierce toward the outside is erupting the pressure full steam; Huang Qu senior official yellow Jinlong earnestly examined without enough time that, then immediately lets the retinue survey group with the most advanced laser analyzer, surveyed has spouted the steam the temperature, the pressure as well as includes other material each data, obtained the data and formerly obtained the data astonishing consistency, in which vapor temperature reached as high as 500 degrees, about the pressure 50 atmospheric pressure, most worrying was in this steam includes some kind of humanity's unknown micro material, possible ambush some kind of unexpected danger, to be needing the further experimental analysis, could obtain the correlation the conclusion!

黄区长官黄金龙进行联络完毕后,就马上按照预先制定的行动计划,带领身边一批精悍的行动队员们,马不停蹄迅速赶到离指挥中心最近的,几乎与椭圆小行星崎岖不平的表面,平直弯曲深入的巨大蒸汽孔洞跟前,只见这个孔洞直径足有200米左右,此时正剧烈的往外喷发着压力十足的水蒸气;黄区长官黄金龙来不及认真查看,便马上让随行探测小组用最先进的激光分析仪,测量了一下喷出蒸汽的温度、压力以及含有其他物质的各项数据,得出的数据和先前得到的数据惊人的一致,其中蒸汽温度高达500度,压力50个大气压左右,最令人担心的是此蒸汽中含有某种人类未知的微量物质,可能潜伏着某种意想不到的危险,需要进一步的试验分析,才能得出相关的结论!

As Coiled Tubing, there are 30 sets of Coiled Tubing Working trucks in China and 30 million yuan ' s (200 thousand meters) requirement. It is broadly used in Tuha, Sichuan, Daqing and Shengli and so on oilfields. Freet ' s Coiled Tubing Working Truck worked in Hekou, Shengli, Zhuangxi, Chunliang and so on oil extraction fields to take the wax-cleaning, sand-washing work and achieved great effect. There are 1,050 sets of working trucks abroad and annual requirement is 1.5 to 2 billion yuan (8 million meters).

对于连续油管,目前国内有30台连续油管作业车,每年需求在3000万元左右(20万米),广泛应用于吐哈、四川、辽河、大庆、胜利等油田,我公司的连续油管作业车先后在胜利油田的河口、胜利、桩西、纯梁等采油厂进行清蜡、冲砂等作业,效果显著;国外有1050台作业车,每年需求量在15-20亿元左右(800万米)。

Among several neutral oils, transformer oil shows the best agglomeration effect. For the complex tailings, in which over 90% of molybdenum distributed in the range of -0.038 mm, the optimal dosage of transformer oil and frother was about 12 kg/t and 0.5 kg/t respectively, and the least agitation time and rate should above 3 min and 400 r/min, respectively.

结果表明,在众多中性油中,变压器油表现出了较好的油团聚浮选效果,对于钼金属分布率高于90%的-0.038mm粒级的浮选尾矿,其合适用量需达到12kg/t左右,与之配合的起泡剂用量在0.5kg/t左右,油团聚时间及搅拌强度分别需达到3min和400r/min以上。

Breed can be used sow law, cuttage law and Qiegenfa to breed, sow ℃ of 20-25 of gemmiparous comfortable Wen Wei to control, spring, summer, Qiu Jun but, left and right sides of a month after sowing is gemmiparous, sow although the law is OK short time acquires more plant amount, but from the time that sows to blossom longer, still be much commonly so with cuttage breed is given priority to, cuttage with spring, summer two Ji Wei is comfortable period, insert with terminal bud for beautiful, cuttage sends a root extremely easily, cuttage hind can become a young plant about 4 weeks.

繁殖可利用播种法。扦插法及切根法繁殖,播种发芽适温为20-25℃左右,春。夏。秋均可,播种后约1个月左右发芽,播种法虽然可以短时间获得较多的植株数量,但是从播种到开花的时间较长,所以一般还是多以扦插繁殖为主,扦插以春。夏两季为适期,以顶芽插为佳,扦插极轻易发根,扦插后约4周即可成苗。

Prices for the furniture range from US$20 to about US$100 for Guidecraft's wide range of wooden pieces, blocks, vehicles and learning toys.

家具类玩具的售价从$20到$100左右不等,售价$100左右的为Guidecraft公司的比较宽泛的领域,像木质拼片,积木,交通工具和学习玩具。

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Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势,并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。

This dramatic range, steeper than the Himalayas, is the upturned rim of the eastern edge of Tibet, a plateau that has risen to 5 km in response to the slow but un stoppable collision of India with Asia that began about 55 million years ago and which continues unabated today.

这一引人注目的地域范围,比喜马拉雅山更加陡峭,是处于西藏东部边缘的朝上翻的边框地带。响应启始于约5500万年前的、缓慢的但却不可阻挡的印度与亚洲地壳板块碰撞,高原已上升至五千米,这种碰撞持续至今,毫无衰退。