工程化学

By Percoll fluid and density gradient centrifugation,the MSC was obtained;after the cells filled the bottom of vessel,subcultured them,when they subculture in third generation ,redigested them,500 R/min centrifugate,alter the

经percoll液密度梯度离心法能获得纯度较高的人骨髓间充质干细胞，这种细胞在体外培养体系中保持旺盛的增殖能力；经轻度离心和化学限定培养基法可诱导骨髓间充质干细胞向成软骨方向分化，增殖旺盛，可传一代培养，是软骨组织工程理想的种子细胞。

Institute of Physical Chemistry,College of Chemistry and Molecular Engineering ,State Key Laboratory for Structural Chemistry of Unstable ＆ Stable Species,Pek ing University,Beijing 100871;Department of Biophysics,The School of Basic Medical Sciences,Peking University,Beijing 100083;Institute of Organic Chemis try,Chinese Academy of Sciences,Shanghai 200032

高峰；沙印林；邱阳；王跃丰；李银玲；来鲁华；吴厚铭北京大学物理化学研究所，化学与分子工程学院分子动态与稳态国家重点实验室，北京 100871；北京大学基础医学院，生物物理系，北京 100083；中国科学院上海有机化学研究所，上海 200032

Objective: To examine the property and proliferation ability of primary culturing MSC, investgate the effect of lightly centrifugation and chemical definition medium to induce MSC differentiate into chondroblast.

目的：了解骨髓基质干细胞原代培养的特性及增殖能力，探讨轻度离心和化学限定培养基法诱导骨髓基质干细胞向成软骨方向分化的作用；为软骨组织工程提供足量、合适的种子细胞。

MSC can be obtained by percoll fluid and density gradient centrifugation, this cells kept proliferation and differentiation ability in vitro; MSC can differentiate into chondroblast by lightly centrifugation and chemical definition medium, after one generation, the differential cells kept the phentype of chondroblast, they are a kind of ideal seed-cells for cartilage tissue engineering.

经percoll液密度梯度离心法能获得纯度较高的人骨髓间充质干细胞，这种细胞在体外培养体系中保持旺盛的增殖能力；经轻度离心和化学限定培养基法可诱导骨髓间充质干细胞向成软骨方向分化，增殖旺盛，可传一代培养，是软骨组织工程理想的种子细胞。

This investigation include two parts: lThe primary culture, chondroblast induction and evaluation of MSC. 2The chondroblast cultured with compound porous materials PDLLA and PDLLA/chitosan.

第一部分人骨髓基质干细胞的原代分离、体外培养、成软骨诱导及鉴定目的：了解骨髓基质干细胞原代培养的特性及增殖能力，探讨轻度离心和化学限定培养基法诱导骨髓基质干细胞向成软骨方向分化的作用；为软骨组织工程提供足量、合适的种子细胞。

Although some of its coal technology, but still not enough to meet the curr 化学工程论文 ent mine development and production needs.

尽管一些煤矿对其进行的技术改造，但仍然满足不了当前矿井发展和生产的需要。

The soybean-protein fiber was developed by Li-Guanqi who is the member of the HuaKang group that is united with chemistry bioengineering in Pu Yang, HeNan province. The producer stuff of the soybean-protein fiber primarily contains beans dregs, hydroxyl and cyanic acid high polymer. Its production principle: First, soaking beans dregs into water and purifying out soybean-protein from the solution.

大豆蛋白改性纤维是由河南省濮阳华康化学生物工程联合集团公司李官奇等研制并生产的一种新型纺织纤维，它的主要生产原料是豆粕、羟基和氰基高聚物，其生产原理是将豆粕水浸分离提纯出蛋白质，将蛋白质改变空间结构并在适当的条件下与羟基和氰基高聚物共聚接枝，通湿法纺丝生成。

1Department of Enterochirurgia, Institute of Traditional Chinese Medicine, Chongqing 400013, China, 2Laboratory of Chemistry, Academy of Chinese Materia Medica, Chongqing 400065, China, 3Laboratory of Basic Medicine Project Research, First Affiliated Hosp

徐月1；秦剑2；周远大3；何海霞31重庆市中医研究院肛肠外科，重庆 400013，2重庆市中药研究院化学研究室，重庆 400065，3重庆医科大学附属第一医院临床药理学研究室，基础医学院生物工程研究室，重庆 400016

In the article modified methods are summarized,including physical modification ,chemical modification ,enzymic modification and bioengineering modification .

本文介绍了大豆蛋白质的改性方法，包括物理改性、化学改性、酶改性和生物工程改性等。

Phase equilibria of fluids and their mixtures are of central importance to chemical engineering.

流体及其混和物的相平衡一直是化学工程中非常重要的问题。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。