工程

Slope; limit equilibrium method; the finite element method; clinometer; displacement; water level; efficiency rainfall; grey system; grey association

工程科技II，工业通用技术及设备，工程基础科学滑坡；极限平衡法；有限元法；测斜；位移；水位；有效降雨量；灰色系统；灰色关联度

As a result, it is an urgent realistic problem that land subsidence induced by tunneling is forecasted and controlled so as to ensure the safety of close-by building and buried pipeline.

因此，如何在隧道施工过程中防止坍塌并可靠地预计和有效地控制施工所引起的地面沉陷以保护工程沿线建筑物和地下管线的安全，已成为城市地铁工程建设中必须解决的一项重要课题。

Furthermore, bad economic loss and social effect can be brought about. As a result, it is an urgent realistic problem that land subsidence induced by tunneling is forecasted and controlled so as to ensure the safety of close-by building and buried pipeline.

因此，如何在隧道施工过程中防止坍塌并可靠地预计和有效地控制施工所引起的地面沉陷以保护工程沿线建筑物和地下管线的安全，已成为城市地铁工程建设中必须解决的一项重要课题。

CMG previously had made private label machinery for the U.S. market for both Cumberland Engineering and Gloucester Engineering.

康联以前已取得私人标签的机制，美国市场为坎伯兰工程和格洛斯特工程。

About HUD: Founded in Guangzhou in 2000, CMG Decoration Design Co., Ltd., after many years of efforts and development, has accumulated rich experience in the design and construction is to a first-class management, high-level...

职位简介：1、三年以上现场工程管理经验，证件齐全。2、熟悉装饰材料及施工工艺，熟悉装修工程管理流程。3、形象好、有良好的沟通协调能力，和强烈的团队协作精神

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

Simulation of the construction of RCC cofferdam in Three Gorges Project is taken as the example by this program, The result are quite good and can be used in the design and the construction of mass concrete structure.

利用本文所研制的有限元程序FEAPTEP对"三峡工程三期RCC围堰施工过程仿真及蓄水安全评价"这一重大项目进行了分析研究，取得了较为满意的效果，为工程设计和施工提供了可靠的依据。

Coll. of Information and Communication Engineering, Harbin Engineering Univ., Harbin 150001, China

哈尔滨工程大学信息与通信工程学院，黑龙江哈尔滨 150001

Information and Communication Engineering Coll., Harbin Engineering Univ., Harbin 150001, China

哈尔滨工程大学信息与通信工程学院，黑龙江哈尔滨 150001

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。