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Results By optimization test, capture antibody and detection antibody (from PVA detection kit, Agdia, USA) were finally diluted at 1:50 (4ug/mL) and 1:25 to be used in one step CBA, respectively. A total CBA reaction time of 2 h was needed. No cross reactivity with other plant virus similar to potato virus Y, lettuce mosaic virus and tomato ring spot virus were found. Detection sensitivity for positive control sample in one-step CBA was 4 folds higher than that in normal plate double antibodies sandwich enzyme-linked immunosorbent assay.

通过实验优化检测条件,最佳捕获抗体工作浓度为4μg/mL、最佳检测抗体工作浓度为1:25倍稀释、最佳反应时间为2h;与马铃薯Y病毒、莴苣花叶病毒、番茄环斑病毒等均未出现交叉反应;阳性样品经64倍稀释后依然可检出,检测灵敏度是传统微孔板ELISA的4倍。

The technique discussed above helps us to determine the distribution of direct singular positions in the manipulators workspace.

本研究使用上述条件找出3PPSR并联式机械手臂正向奇异位置在工作空间的分布,并发现此机构在工作空间内任何区域皆有正向奇异位置。

Because of the tetrode working in severe condition, such as high voltage over to 13.5kV, the whole system must be under control. This system acquires the corresponding electric parameter of the tetrode constantly during the process of measuring.

此系统在检测的过程中,连续采集四极管相应的电参数,如果相应管件不在额定的条件下工作时,则发出声音及视觉警报,向现场工作人员提出警示,并加以保存。

Price : Mode of payment : irrevocable pay ordervia SWIFT or by irrevocable conditional money transfer MT 103 SWIFT, within five bank working days after reception of signed contract and receipt of the per advice payment to be released into applicant's account within five working days banking day after receipt, verification and authentication of the contracted delivery of each and every trenched SBLC.

价格:付款方式:不可撤销支付令通过SWIFT或不可改变有条件汇钱MT103系统迅速,在五个银行工作日内接收签订的合同和收据的,每发放意见被释放到申请人的户口在五个工作日内银行当日收到后,核查和认证的合同规定的交付每个槽式储能产生高射频峰值。这么多字'''汗

Size measured at the shoulder--Males 25 to 27 inches; females 23 to 25 inches. Oversized dogs should not be penalized when general soundness and proportion are in favor. Penalize undersize.

首先,黑褐猎浣熊犬的本质是一种工作犬,一种追踪和将猎物赶到树上去的猎犬,需要他在工作时,能够抵御冬季的严寒和夏季的炎热,并适应复杂的地形条件。

In this paper, the space-time finite element theory and its application technologies are studied and discussed. Furthermore the space-time finite element method is used to analyze the transient characteristics of electromagnetism equipments. The work is important to develop the numerical method for the transient electromagnetic field. The detailed work is as follow.In the basis theory of finite element method used in the transient engineering problem, the functional theory and the finite element theory are applied to studied the existence condition of a generalized variation principle, which is the basis of the space-time finite element method.

本文在完善时空有限元法的基础理论和应用技术并将该方法应用于解决电磁装置的瞬态过程分析等相关方面进行了深入的研究与探讨,该工作对于发展瞬变电磁场问题的数值计算方法具有重要的意义,为此进行了如下几方面的工作:在工程瞬变问题的有限元方法的基础理论方面,本文应用泛函理论和有限元理论,系统讨论了一般工程瞬变问题的变分定理的存在条件及对应的变分定理。

First, i want to introduce myself, my name is Zhangsan,I am 24years old,from a small city which in the south of China,,in 2007 ,I was graduate from nomal university,get the baccalaureate of law.After my graduated,I once did some selling work, but I still want to do some work in law, but in China, that the students who's major was law was very difficult for get job,it is quite diffrent from your weatern country.

我先向你自我介绍下,我叫张三,今年24岁,来自中国南方的一个小城市,2007年毕业于一所普通的大学,拿到了法学学士,毕业后做过一段时间的销售工作,我一直想从事法律方面的工作,但是在中国教育背景下,法律专业的就业前景不好,这不同于你们西方国家,所以我早就萌发了出国继续深造攻读硕士的念头,但是由于英语水平太差和家庭条件

First, i want to introduce myself, my name is Zhangsan,I am 24years old,from a small city which in the south of China,,in 2007 ,I was graduate from nomal university,get the baccalaureate of law.After my graduated,I once did some selling work, but I still want to do some work in law, but in China, that the students who's major was law was very difficult for get job,it is quite diffrent from your weatern country. so long ago, I have been get a plan for go abroad to get a master's degree in law. but for my poor English and economy,I think it is very hard to me.

我先向你自我介绍下,我叫张三,今年24岁,来自中国南方的一个小城市,2007年毕业于一所普通的大学,拿到了法学学士,毕业后做过一段时间的销售工作,我一直想从事法律方面的工作,但是在中国教育背景下,法律专业的就业前景不好,这不同于你们西方国家,所以我早就萌发了出国继续深造攻读硕士的念头,但是由于英语水平太差和家庭条件不好,感觉不太现实,一直没有去争取。

Certainly, the applications of our results are constrained because of the using of some approximation. The tag discloses this point and several subjects that are worth, we considering, continuing to be investigated on the EOSs and anharmonic properties of solids.

当然,本文研究工作的结果要在一定的宏观条件下才能适用,并不是无条件普适的,因此就此进一步开展工作将是十分有意义的,结束语中指出了对EOS及非谐性质的研究我们认为值得继续探讨的课题。

TheBlack and Tan Coonhound is first and fundamentally a working dog, atrail and tree hound, capable of withstanding the rigors of winter, theheat of summer, and the difficult terrain over which he is called uponto work.

首先,黑褐猎浣熊犬的本质是一种工作犬,一种追踪和将猎物赶到树上去的猎犬,需要他在工作时,能够抵御冬季的严寒和夏季的炎热,并适应复杂的地形条件。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。