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Fund Project: the National Natural Science and Technology Source Program, No. 2001DEA1006*Abstract: Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have no cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少,从细胞形态来看神经干细胞为圆形或椭圆形,无或有较短的突起,核质比大,核染色较深,形态上与其它种类的细胞没有明显的差异,并且未找到一种细胞表面特异性标志物,因此目前鉴定神经干细胞主要有以下3个方面:特异性神经抗原的表达,包括巢蛋白、Musashi、转录因子及细胞黏附分子;自我更新能力,包括单细胞克隆分析、BrdU标记S期细胞;多向分化潜能,包括免疫细胞化学法、聚合酶链反应色法。

Number of neural stem cells is small. NSCs look like circle or ellipse with or without short neurite, with large nuclear-cytoplasmic ratio and deep karyotin. NSCs have no visible differences with other kinds of cells in appearance, and have cell surface marker. Currently, there are mainly three aspects to identify NSCs: expression of specific nerve antigen, including nestin, Musashi, transcription factor, and cell adhesion molecules; self-renewal ability including single cell clone analysis, BrdU mark and S phase cell; the potential of multi-direction differentiation including immunocytochemical process and reverse transcriptase polymerase chain reaction.

由于神经干细胞的数量很少,从细胞形态来看神经干细胞为圆形或椭圆形,无或有较短的突起,核质比大,核染色较深,形态上与其它种类的细胞没有明显的差异,并且未找到一种细胞表面特异性标志物,因此目前鉴定神经干细胞主要有以下3个方面:特异性神经抗原的表达,包括巢蛋白、Musashi、转录因子及细胞黏附分子;自我更新能力,包括单细胞克隆分析、BrdU标记S期细胞;多向分化潜能,包括免疫细胞化学法、聚合酶链反应色法。

A tree whose hungry mouth is prest againsr the earth's sweet flowing breast; a tree that looks at god all day, and lifts her leafy arms to pry; a tree that may in summer wear a nest of robins in her hair; upon whose bosom snow has lain; who intimately lives with rain.

一株树,他的饥渴的嘴吮吸着大地的甘露。一株树,他整日望着天高擎着叶臂,祈祷无语。一株树,夏天在他的发间会有知更鸟砌巢居住。一株树,白雪躺在他胸上,他和雨是亲密的伴侣。

A tree whose hungry mouth is prest againsr the earth's sweet flowing *; a tree that looks at god all day, and lifts her leafy arms to pry; a tree that may in summer wear a nest of robins in her hair; upon whose bosom snow has lain; who intimately lives with rain.

我向,永远不会看到一首诗,可爱的如同一株树一样。一株树,他的饥渴的嘴吮吸着大地的甘露。一株树,他整日望着天高擎着叶臂,祈祷无语。一株树,夏天在他的发间会有知更鸟砌巢居住。

Bees hold commitee meetings to discuss new nesting sites, flocks of quelea learn from each other where to find the best food, fire ants even use their own bodies to form a raft and escape when their nest is destroyed by floods.

Bees委员会举行会议,讨论新的筑巢地点,成群的红嘴奎利亚互相学习在哪里可以找到最好的食物,甚至用火蚁他们对自己的身体形成一个木筏逃生时,它们的巢是由floods。

As a member of septation initiation network, sepH is a key factor of cytokinesis in Aspergillus nidulans. However, how the suppressors of sepH affect cytokinesis is not yet understood.

在构巢曲霉Aspergillus nidulans的细胞隔膜开始网(septation initiation network,SIN)的信号途径中,sepH基因对胞质分裂起着关键的正调节作用,但对该途径中同样起着十分重要作用的有关反向调节子的研究目前还不清楚。

For the purpose of environment protection and reasonable development of scale breeding, Juchao District in Anhui Province, which is situated by the middle of Chaohu Basin, was taken to be studied. The maximum waste load of farmland was estimated through the breeding numbers and respective excretive coefficients. By comparing the actual load of livestock manure with the maximum admissible load, environmental risk of actual breeding scale in each community was evaluated.

以巢湖流域内的安徽省居巢区作为研究区域,结合当地规模化养殖小区的畜禽饲养量、排污量以及有效耕地面积,对畜禽粪便的耕地最大污染负荷量进行了估算,并与当地畜禽粪便的实际负荷量进行比较,对现有养殖规模条件下的环境风险进行了评价。

The 600,000-acre (242,812-hectare) forest boasts seemingly endless stands of longleaf pines and is home to the endangered red-cockaded woodpecker, whose designated nest trees are identifiable by the white band painted around their trunks.

60万英亩( 242812公顷)森林拥有似乎无休止的看台长叶松树是家园中的濒危动物红色cockaded啄木鸟,其指定的巢树是可识别的,由白色带子描画的周围的树干。

Two specific hypotheses that availability of high quality food, predation and interspecific competition have independent and addictive effects on population system dynamics for microtine small mammals; and that availability of high quality food could reduce home range and aggressive behavior level between individuals of microtine small mammals, predation and interspecific competition have independent effects on spatial behavior of microtine small mammals were generally supported by the results in this study.

研究结果检验了本项研究提出的特定假设:高质量食物可利用性,捕食及种间竞争对田鼠类小型哺乳动物种群系统动态具有独立的和累加的效应;高质量食物可利用性能降低田鼠类小型哺乳动物的攻击行为和巢区,捕食与种间竞争能减少田鼠类小型哺乳动物的活动。

This picture of how cancer cells shift between two alternating states -- travelers and nesters -- represents a new understanding of how cancer metastasizes, or spreads to other parts of the body, said the Duke Comprehensive Cancer Center researchers who conducted the study.

&该研究描绘出癌细胞如何在两种交互的状态下转变-迁移态和成巢态。这表明对于癌细胞是如何转移或扩散到人体其他部位的机制有了新的认识。&实施该项研究的杜克综合肿瘤中心的研究人员说。

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Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

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There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。