巢的

A complete bovine 18ku-bFGF gene was cloned by nested-PCR and subcloned into expression vector pET-28a.The recombinant plasmid pET-28a-bFGF was transformed into Escherichia coli BL21.At 25℃,recombinant protein was induced by 0.5mmol/L IPTG for 5 hours.Supernatant of cell lysate was purified using Ni-NTA method and the products was submitted to detect the bioactivity by Western-blotting,which indicated that the fusion protein contained recombinant bovine bFGF.Results showed that recombinant bovine bFGF was produced and solubly existed in the supernatant.NIH-3T3 fibroblasts were used to detect the bioactivity of the fusion protein.

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列，并构建了原核表达载体pET-28a-bFGF，将其转化大肠杆菌BL21，在25℃低温条件下，用0.5mmol/L IPTG诱导表达5h，用Ni-NTA亲和纯化细胞裂解上清液，经Western-blotting检测，结果显示，在特定的诱导条件下，重组牛bFGF基因在大肠杆菌中获得了表达，并且主要以可溶性状态存在于细胞中。

Type genus of Terebellidae; tube-forming marine polychete worms with many filamentous tentacles.

蛰龙介科的模式属；有许多丝状触手的海产菅巢多毛类蠕虫。

The Third World Action Group accused Nestle of unethically persuading nervous mothers of newborns that bottle feeding was best for their babies.

第三世界行为组织起诉了雀巢公司，并且规劝新生儿的母亲，母乳喂养是对新生儿来说最健康的哺乳方式。

Levels of soluble markers that associate with peripheral mobilisation and homing were studied in the serum samples of all subjects.

采集所有参与者的血标本测定与外周动员和归巢有关的可溶性标志物水平。

It may be a transitory situation, as the Eagles squad does not look equipped to last the distance, but why take the fun out of the job of headline writers, those molehill-into-a-mountain merchants who are already conjuring up "FantaLazio"(wow, that must have required some time to think up) and the like?

虽然形势还并不明朗，因为鹰巢似乎还没有准备好将这种态势保持到赛季末，但是对于那些撰写大标题的人来讲，这个现象可是乐趣多多的，他们挖地三尺，费了很大的劲终于杜撰出&FantaLazio&这个称谓来（哇塞，看起来他们一定费了不少劲）。

Then we see them go to sleep together in their nest for the night.

然后我们在他们的夜的巢看见他们去一起睡。

Baidu's initial motive for developing the platform had been to make more money from auctioning search terms.

百度开发&凤巢&平台的最初动机是，从拍卖搜索词的业务中赚取更多利润。

Admiring nature's handiwork, Dave Stomberg basks in the glow of a frame full of honey.

戴维 斯通伯格钦佩这大自然的艺术品，他在阳光下一块布满蜂蜜的巢框。

That's a partial reason behind the cerebrates' merging into a new, singular Overmind during the early events of Brood War.

这也是为什么在母巢之战中脑虫们融合成一个新的主脑的原因。

So people like the idea of nests and cocoons, too.

因此，喜欢关于巢和茧的人的想法亦是如此。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。