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Results: The positive detection ratio of IHC and nested PCR were 64.2% and 81.1%, respectively. It was showed that the positive detection ratio of nested PCR was significantly higher than that of IHC (P.01). The nested PCR assay had advantages over IHC on sensitivity, specificity, Youden's index, positive predictive value and negative predictive value.

结果:免疫组化检测和式PCR检测的阳性检出率分别为64.2%和81.1%,式PCR技术检测肾癌组织支原体在灵敏度、特异度、约登指数、阳性预测值和阴性预测值方面均优于免疫组化技术。

A classic example is noisy begging by nestling songbird when a parent returns to the nest with food.These loud cheeps and peeps might give the location of the nest away to a listening hawk or raccoon, resulting in the death of the defenseless nestlings.

一个经典的例子就是当成鸟带着食物归里的雏鸟就会大声的乞食,这些叽叽喳喳的声音可能就会将穴的位置暴露给听到这些声音的鹰隼或是浣熊,导致毫无防备的雏鸟死亡。

The honeycombs are fi lled with lots of small hexagon spaces that the bees have built of bee wax.

每个蜂都是六角形的小空间,蜜蜂使其空间的利用率达到最大,称为室,蜜蜂将蜂蜜存放在室中。

Colonial breeding provides birds several advantages, such as mobbing, information- centre, and enhancing feeding efficiency. However, it might also bring some disadvantages, such as increasing chances of attracting predators, intra-specific parasite, nest-parasite and infectious diseases.

行集体营具有如共同攻击天敌、资讯交换、增加群体觅食效益等好处,但同时也具有吸引天敌、种内托卵寄生、寄生虫及疾病传染等缺点,因此在不同大小的生殖群聚及生殖群聚内的不同位,对於其生殖表现都可能造成影响。

There is a rare primary liver tumor that has been reported as ossifying stromal-epithelial tumor (3 cases), desmoplastic nested spindle cell tumor (4 cases), and nested stromal-epithelial tumor (6 cases).

肝脏有一种罕见的原发性肿瘤,先前分别有文献将其报道为骨化性间质-上皮肿瘤(3例)、促纤维组织增生性状梭形细胞肿瘤(4例)和状间质-上皮肿瘤(6例)。

Teng Hsiao-ping, you, too, have a nest , and if I destroyed it, wouldn't you make a few squawks?

邓小平你也有一个,我把你的搞烂了,你要不要叫几声?

All experiment animals lived well with no death or malignant arrhythmia following cell transplantation. Frozen sections of the heart showed a few blue-stained cells under the fluoroscope in the cell transplantation group 4 weeks later. This indicated that stem cells were successfully homing. Using hematoxylin-eosin staining, homing cells were distributed over the infarct zone, with the presence of single or mass. In the model control group, no blue-stained cells were determined.

细胞移植后两组大鼠均存活良好,未出现死亡及恶性心律失常,4周后细胞移植组心脏冰冻切片荧光镜检显示心肌内有少量胞核蓝染的细胞,提示干细胞成功归,结合苏木精-伊红染色,归细胞位于心肌梗死灶周边区域,呈单个或小团聚集;而模型对照组心肌切片未见胞核蓝染的细胞。

We biopsied 1-2 single blastomere from 6-8 cell cleavage stage intracytoplasmic sperm injection embryo, and using nested PCR amplified the high frequently mutation region G380R of FGFR3 gene of the single blastomere. The products of PCR were digested by restriction enzyme Bfm Ⅰ, then the digested products were detected by 10% PAGE to see whether the embryo inherted the mutation of the patient and to screen out normal embryo transfer.

活检经胞质内单精子注射(intracytoplasmic sperm injection,ICSI)授精的胚胎发育至6~8细胞期的1~2个单卵裂球,采用式PCR扩增单卵裂球的FGFR3基因的高发突变位点G380R区域,用限制性内切酶BfmⅠ消化式PCR的内扩增产物,再经10%聚丙烯酰胺凝胶电泳检测有无遗传患者的该种突变,从而筛选出正常胚胎移植。

The brickcaptured eagle d trap nest.

小鹰很喜爱那个,自出生以来那个那是它所有的天地。

The primers and the detection programs have been used to detect the pathogens in the inoculated-diseased cucumber plants .The details are reported as follows: 1. By using the primers ITS1/ITS4, only Fusarium isolates could be amplified to produce a special fragment of 750bp. This product generated 480bp, 200bp and 100bp fragments after digested by restriction enzyme HinfⅠonly from the pathogen of Cucurbits Fusarium Wilt.

引物ITS1/ITS4只在镰孢属菌株上扩增到一条750bp分子片段,其他供试菌株无扩增条带产生,扩增产物经HinfⅠ酶解后,只有瓜类尖镰孢菌产生480bp、200bp和100bp 3条带,说明该PCR-RFLP程序对瓜类尖镰孢菌具有特异性;式PCR引物BIK1/BIK4和BIK2/BIK3只有在黄瓜尖镰孢菌菌株上扩增到一条800bp分子片段,其他菌株均无扩增条带产生,说明该式PCR程序对黄瓜尖镰孢菌具有特异性。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。