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The largest diameter of the tumor was 10 centimeters. By light microscopy the tumor was composed of two parts. Main part consisted of small round or polygon tumor cells with an island or nestlike arrangement of organic structure. The other showed moderately differentiated characteristics.

光镜下肿瘤由两部分组成，大部分由大小一致的圆形、多角形小细胞组成，细胞排列成岛状、巢状等器官样结构；另一部分呈中分化腺癌形态特点。

AIM:To investigate the expression of Nestin in human neuroepithelial tumors and to evaluate the biological characteristics of Nestin positive tumor stem cells in vitro and the significance.

目的：检测巢蛋白在神经上皮肿瘤组织、细胞中的表达，评价Nestin+细胞体外生物学特性并探讨其意义。

Methods The neuroepithelial stem cells were isolated and cultured by microdissection, mechanical blowing and serum-free suspension culture.

方法采用显微解剖、机械吹打、无血清悬浮培养方法分离培养神经上皮干细胞，采用巢蛋白免疫细胞化学染色技术检测神经上皮干细胞，用NSE和GFAP免疫组化染色检测并计数神经细胞和神经胶质细胞。

Results: Neuroepithelial stem cells derived from the embryonic neural tube could expand into neurospheres in the free serum medium and express nestin.

结果： GFP转基因鼠神经管来源的神经上皮干细胞在无血清培养基内能增殖形成神经球，呈巢蛋白阳性。

DNA methyltransferases, homing endonucleases, nicking enzymes, specificity subunits and control proteins are also included.

还包括DNA甲基转移酶、趋巢性内切核酸酶、切割酶、专一亚单位和控制蛋白质。

Cytolysosomes increased,cellular nucleus became twisted,rough endoplasmic reticulums swelled,and chondrosome became vacuolization.Dead intervertebral disk cells were located in ?nidi? which were made of multilayer degenerative collagen fibers.

术后8周实验组椎间盘开始出现大量死亡细胞，表现为溶酶体明显增多，细胞核扭曲，粗面内质网扩张，线粒体空泡化，死亡细胞外周的&巢&增厚呈多层排列，致密颗粒增多。

Working with geese, he rediscovered the principle of imprinting in the behavior of nidifugous birds.

在对孵化后离巢鸟的研究中，他重新发现了胚教原理。

Following their infusion into nonirradiated animals, hMSCs homed at a ery low leel to arious tissues and no significant engraftment was found in other organs.

在未予照射的小鼠中，输注的hMSC归巢到肺部、骨髓、肌肉等组织的数量很少，在其他器官亦无明显的植入。

Results Risk for lung cancer caused by exposure to nitrosoamine increased significantly after adjustment of nonoccupational risk factors (RR=271,with 95% CI of 132 to 557),with a significant doseresponse relationship between exposure to nitrosoamine and lung cancer.

结论（1）本文数据提示病例－队列分析因标准误较小、抽样更简便而优于巢式病例－对照研究方法；（2）亚硝胺暴露与肺癌之间有较确定的关系，但受到吸烟和经济状况等因素作用的修饰，需进一步研究

Blood samples were obtained from 5 patients with AIDS, the C2~C3 region of HIV-1 envelope gene was amplified by nested polymerse chain reaction from plasma of each patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous and nonsynonymous substitutions as well as ds/dn ratios were calculated.

采用巢式PCR方法对5例艾滋病患者血浆HIV-1env基因C2~V3区进行PCR扩增，产物经纯化后克隆至pMD-18T载体中，每例患者分别挑取5~8个克隆，共34株，完成阳性克隆的鉴定、抽提纯化和测序，将获得的DNA序列及氨基酸序列进行分析，并计算其氨基酸同源替换率与非同源替换率的比值。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。