嵴

Results There was a similar distributive pattern of Neul, PPCA and β-gal in the inner ear. Neul intense staining was observed in the cochlear spiral ganglion cells, spiral limbus, spiral ligament, vestibular ganglion cells, cristae, maculae hair cells, and weak staining in inner hair cells, outer hair cells, supplying cells of the organ of Corti and stria vascularis. The intense staining of PPCA and β-gal were observed in the spiral ganglion and vestibular ganglion cells, and weak staining in the spiral limbus, spiral ligament, stria vascularis and organ of Corti. The inner ear exhibited no staining when Neul, PPCA and β-gal were deficient, respectively.

Neul最强的染色主要在螺旋神经节细胞、螺旋韧带、螺旋缘、前庭神经节细胞及壶腹嵴、球囊和椭园囊感觉毛细胞，较弱的染色分布于血管纹和Corti器内、外毛细胞及支持细胞；PPCA和β-gal在螺旋神经节和前庭神经节细胞有较强的染色，血管纹、螺旋韧带、螺旋缘和Corti器内、外毛细胞及支持细胞呈较弱的染色反应；各自酶缺乏时内耳免疫染色消失。

Objective: To determine whether the cells that we obtained from the in vitro culture of human embyonic genital ridges and dorsal mesenteries were the undifferentiated human embryonic germ cells , to find out the best kind of passaging method , and finally to identify these serial passaged cells .

目的：鉴定组织块培养法体外培养人胚胎生殖腺嵴和背侧肠系膜所获得的细胞为未分化的人胚胎生殖细胞；尝试不同时间和不同方法对人EG细胞进行传代培养，探讨最佳传代时机和传代方法；并确定传代培养的细胞仍为未分化的人EG细胞。

Methods: The hEGcs from the gonadal ridges and dorsal mesenteries of human embryos aged 5-10 weeks were cultured with or without cytokine in culture medium to observe their growth and the effects of different cell culture systems were compared.

取5~10周人胚胎生殖腺嵴和背侧肠系膜，用添加和不添加细胞因子的两种培养体系对比进行组织块原代及传代培养，选取生长情况良好的传至第4代的人EG细胞，移植到急性心肌梗死大鼠的梗死心肌周围，分别于移植后1天、1、2、4周处死大鼠，以鼠抗人细胞核抗体MAB1281作为示踪剂，通过免疫组化方法检测移植细胞的分布。

To isolate, cultivate and identify the human embryonic fibroblasts derived from the gonadal ridges and dorsal mesenteries of human embryos, and to detect the expression by hEFs of cytokines crucial for the growth of human embryonic germ cells in vitro.

目的：从人胚胎生殖腺嵴和肠背系膜中分离、培养及鉴定人胚胎成纤维细胞，检测hEFs表达人胚胎生殖细胞体外生长所需的重要细胞因子。

The hEFs derived from gonadal ridges and dorsal mesenteries of human embryos are successfully isolated and cultivated,and the cells express the cytokines essential for the growth of hEG in vitro.

成功地分离和培养了人胚胎生殖腺嵴和肠背系膜来源的成纤维细胞，证明其表达对于hEG体外生长起重要作用的细胞因子。

Methods Primary human embryonic germ cells were obtained by tissue culturing of embryonic genital ridges and mesenteries from the embryos aged 8 weeks, and then were passaged on the 8th and 16th day using pick-up and digestive passage method. Three kinds of digestive juice were used.

取8周人胚胎的生殖腺嵴和肠背系膜，进行组织块体外培养人胚胎生殖细胞，选择原代培养第8天和第16天的细胞，采用挑取传代法和消化传代法，其中的消化传代法采用三种不同的消化液进行传代培养，记录各代培养的细胞集落数。

Methods The hEFs were isolated by enzyme digestion from gonadal ridges and dorsal mesenteries of 5- to 9- week old human embryos. The cells were then cultivated. The biologic characteristics (morphology, growth characteristics and cell cycle) of these cells were also studied.

利用酶消化法从孕5～9周龄人胚胎的生殖腺嵴和肠背系膜中分离培养hEFs，检测其生物学特性（细胞形态、细胞生长特点、细胞周期）。

Methods The hEFs were isolated by enzyme digestion from gonadal ridges and dorsal mesenteries of 5-to 9-week old human embryos.

利用酶消化法从孕5~9周龄人胚胎的生殖腺嵴和肠背系膜中分离培养hEFs，检测其生物学特性(细胞形态、细胞生长特点、细胞周期)。

Methods: the hegcs from the gonadal ridges and dorsal mesenteries of human embryos aged 5-10 weeks were cultured with or without cytokine in culture medium to observe their growth and the effects of different cell culture systems were compared. the hegcs' from passage 4 were transplanted directly to infarcted myocardium.

取5~10周人胚胎生殖腺嵴和背侧肠系膜，用添加和不添加细胞因子的两种培养体系对比进行组织块原代及传代培养，选取生长情况良好的传至第4代的人eg细胞，移植到急性心肌梗死大鼠的梗死心肌周围，分别于移植后1天、1、2、4周处死大鼠，以鼠抗人细胞核抗体mab1281作为示踪剂[1]，通过免疫组化方法检测移植细胞的分布。

Methods:Human embryonic tissues containing genital ridges and dorsal mesenteries were isolated and then cultured in 5 different patterns: simple mechanical disaggregation,trysinization, combination of the former 2 and with/without feeder cells.

体外分离人胚胎生殖嵴和肠系膜组织，按单纯机械分离、酶消化分离、二者结合法及是否有饲养层将组织分为 5种不同的方式培养后，用碱性磷酸酶标记，计数阳性克隆并进行比较。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。