嵌合性

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗；(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力；(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性；(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡；(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型，应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况，明确其靶向性；(6)将荷瘤裸鼠分3组进行放射免疫治疗，分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

However, mosaicism of both CGG repeatlength and methylation status exists in fragile X patients.

全突变经常是嵌合性的，甲基化现象也会嵌合发生。

The new chimeric infectious DNA clone and its derived, a virulent chimeric virus are constructed from the nonpathogenic PCV1 in which the immunogenic ORF gene of the pathogenic PCV2 replaces a gene of the nonpathogenic PCV1, preferably in the same position.

所述新型嵌合的传染性DNA克隆及其衍生的有毒力的嵌合病毒是用非致病性PCV1构建的，其中，致病性PCV2的免疫原性ORF基因取代了非致病性PCV1的基因，优选是在相同的位置上取代。

The results indicated that:The multi\|locus DNA fingerprint with a new synthesized probe\|JL\|02,has enough polymerism and good stability,and should be very useful to monitor the chimerism in different tissues of ES cell chimeric mouse and to check whether an ES cell line has the capacity to enter the germ line,especially when involving strains that can not be discerned with coat color or biochemical markers.

结果表明：采用新型的人工合成的寡核苷酸多聚体探针(JL-02探针)的多位点DNA指纹图谱，具有足够的多态性和很好的稳定性。适用于鉴定ES细胞在嵌合体小鼠各种组织器官中的嵌合情况，也适用于鉴定ES细胞是否具有种系嵌合能力，尤其适用于在嵌合体研究中，最适宜的品种组合具有相同的毛色或相同的酶型。

Discrete TSPs for lethality are localized on the first, the second and the third larval instar for a 10 hr period after pupation with an interval of a few hours between TSP and the lethal phase.

对于成虫盘缺失和重复的关系主要在48小时为一个脉冲的热处理盘中进行，对dl基因的表达采用了遗传嵌合性的研究，其结果如下：1。

To develop safe and effective vaccines is the fundamental strategy to combat the fatal disease. Among the HIV vaccines in research, live attenuated vaccine is attractable since it can induce broad, long-lasting and strong immunity.

采用HIV与猴免疫缺陷病毒(SimianImmunodeficiency Virus，SIV)构建的SHIV嵌合病毒为开发HIV疫苗提供了一个新方向，但由于SIV与HIV的同源性很高，二者嵌合的SHIV在安全性方面存在一定的风险。

Methods:The subjects were 55 female recipients of cadaveric renal transplantation,whose donors were male.

近年来，通过对器官移植后长期存活患者末梢血的研究，发现其中有来自供体的细胞存在，即所谓的末梢血嵌合性〔1〕。

A chimeric primer was designated in which segment A near 3' end is complementary to HB plus strand just before the DR2 region gap, and segment B near 5' end is consensus to part of the human immunodefficient irus genomic sequence, without homogenetic relationship to HB genome.

设计一嵌合引物，使之3'端的A片断与HB正链互补，并恰好位于DR2缺口之前；5'端的B片断与人类免疫缺陷病毒的基因序列具有同源性，与乙型肝炎病毒的基因组无同源性。

Results: Human stem cells were successfully transplanted into 6 fetal sheep and 5 lambs were born and lived. PCR analysis showed that there was human derived cell engraftment in the liver of the 5 lambs. Immunohistochemical staining and ISH with Alu probe showed human derived hepatocytes in the lobes or around periportal area, expressing albumin and CR18. Alu ISH also showed there were positive cells in the epithelia of bile duets.

结果：在移植的 6 只胎羊中，5 只顺利生产；PCR 检测显示 5 只实验羊肝脏组织中均有人源性细胞的嵌合：免疫组化及 Alu 探针原位杂交显示人源性细胞分布於门管区周围和肝小叶中，表达白蛋白、CK18 等肝细胞分化标志；Alu 探针阳胜细胞也分布於胆管上皮中。

A protocol for nonmyeloablative allogeneic hematopoietic stem-cell transplantation that includes total-body irradiation and treatment with alemtuzumab and sirolimus can achieve stable, mixed donor-recipient chimerism and reverse the sickle cell phenotype.

包括全身照射以及阿仑单抗和西罗莫司治疗在内的非清髓性异基因造血干细胞移植方案，可取得稳定的混合供者—受者嵌合性，并且可逆转镰状细胞表型。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。