嵌合体

The gene targeted mice are commonly produced by breeding the heterozygous targeted mice following microinjecting the homologous recombinant ES cells into the blastocoele cavity of 3.5dpc embryos at the blastocyst stage to give birth to chimeric mice.

传统基因敲除获得某一突变基因的纯合体动物首先要利用打靶胚胎干细胞获得嵌合体动物；随后用嵌合体动物与野生型个体交配得到突变基因的杂合体动物；最后才能通过杂合体动物之间的交配来生产纯合体动物。

This article reports the experimental results about y-ray induced chimaera of awned mutation in Mt generation of Triticum aestivum L.

在辐射诱变的实践中通常认为：M_1代所有的新的突变都将是杂合体，本文报道了用r-射线诱发长芒突变嵌合体的试验结果，并对嵌合体进行了遗传分析，结果表明：M_1代嵌合体的长芒突变是一种隐性基因突变，它可以是纯合突变。

The results indicate that day 5 p.c.primitive ectoderm cells are able to participate in embryogenesis after be-ing introduced into day 4 p.c.blastocysts and result in the production of adult chimeras.

嵌合体与C_(57)BL/6 J小鼠交配后所得的结果表明，原始外胚层细胞在嵌合体内能形成有功能的配子。上述结果说明，原始外胚层细胞与内细胞团细胞、体外培养的胚胎干细胞一样，具有发育全能性。

The leaves at physiological age of 20 days on the medium of MS+0.4 mg/L 6-BA+0.01 mg/L IBA reached the maximum inductivity of chimera buds,or 34.8%.

生理年龄为20 d的叶片，在MS+6-BA 0.4 mg/L+IBA0.01 mg/L 培养基中，嵌合体诱导率最大达到34.8%，暗培养会降低嵌合体的诱导率。

Chimera2 and 4 were similar to the langshan chickens separately on the performance of dustbathing and standing, while the behavioral pattern of the other three chimeras were close to the recipient.

比较发现，嵌合体1的这些行为的发生频率都位于狼山鸡相应行为平均值的95％的置信区间之內，其行为模式倾向于供体狼山鸡，嵌合体2和6分别在洗浴和站立行为的发生频率上与供体狼山鸡相似，而其他3只嵌合体鸡的行为模式却与受体AA肉鸡接近。

The results indicated that:The multi\|locus DNA fingerprint with a new synthesized probe\|JL\|02,has enough polymerism and good stability,and should be very useful to monitor the chimerism in different tissues of ES cell chimeric mouse and to check whether an ES cell line has the capacity to enter the germ line,especially when involving strains that can not be discerned with coat color or biochemical markers.

结果表明：采用新型的人工合成的寡核苷酸多聚体探针(JL-02探针)的多位点DNA指纹图谱，具有足够的多态性和很好的稳定性。适用于鉴定ES细胞在嵌合体小鼠各种组织器官中的嵌合情况，也适用于鉴定ES细胞是否具有种系嵌合能力，尤其适用于在嵌合体研究中，最适宜的品种组合具有相同的毛色或相同的酶型。

This paper includes the following aspects: the concept of chimerism, type, methods and the establishment of the mechanism of immune tolerance induction, the chimeric detection and its clinical application of a research progress.

本文就嵌合体的概念、种类、建立嵌合体的方法及其诱导免疫耐受的机理，嵌合体的检测及临床应用的研究进展作一综述。

After 6 to 7 days of culture, Eleven ES cell lines were selected and explanted. At last we obtained 4,3,4 and 3 ES cell lines separately. One of the B6C3F_1×B6D2F_1 ES cell lines was selected to future study its competent for germline transmission.We founded the ES cell line has normal karyotype on the chromosome analyse.In teratoma experiment by injected ESCs into C57 mices inguen, we gained teratoma contained cells come from all three embryonic germ layers.

选择一株具有高度杂合背景的B6C3F_1×B6D2F_1 ES细胞系进行核型分析、畸胎瘤实验后与ICR品系2n胚胎构建嵌合体并得到具有生殖系传递能力的嵌合体小鼠，证明此细胞系具有生殖系嵌合能力。

These ES cells were introduced into host embryos from outbred KMW though blastocyst injection. Using GFP, the attractive genetic marker, we have been able to follow the fate of ES cells in living embryos and to observe directly the contribution of these cells to mouse embryos. Totally, four GFP-expressing 'green' chimeric mice expressing GFP were obtained.

为研究嵌合体动物中供体胚胎干细胞在宿主胚胎发育中的走向和定位，同时探讨绿色荧光蛋白基因作为报告基因在转基因动物制作中的应用价值，本研究将pEGFP-N1基因导入小鼠ES-D3细胞系，得到稳定表达GFP的胚胎干细胞亚系ES-D3-GFP，通过对昆明小鼠的囊胚腔注射，获得了4只表达绿色荧光蛋白的嵌合体小鼠。

The fourth generation of transgenic medaka,inbred variety,was obtained by heterozygote (F2 and F3) breeding of two generations and homozygote (F4) breeding of one generation based on mosaic (F1) of transgenic medaka.

对嵌合体的转基因青鳉（F1）进行了两代的杂合体（F2和F3）筛选和一代的纯合体（F4）筛选，获得第4代转基因青鳉纯系品种。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失