嵌合

Now, they are standing in a splitting gap, jumping from one block to anothe, growing up with every step in company with the formation of a new block, every link embeded in times advancing wheels.

他们的每一步成长都伴随着新的板块的形成，每一个环节都细微地嵌合在时代前行的车轮中。

This done, shetried to work out whether chimerism influenced parental care in marmosets.

在此发现之后，罗斯博士又尝试了研究嵌合现象是否会对狨猴双亲关爱有任何影响。

The results indicated that the c-terminal fragment of Arabidopsis CRY1 gene was successfully integrated into the rape genome, mainly in the form of tabling, with a few in the form of single copy. The transformed gene could be stablytransmitted in the progeny, with a transmitted line rate of 62.5%. The genetic pattern of the transformed gene in the T_2 generation was mostly non-Mendelian. The transgene was expressed in some of the transgenic plants, but kept silent, in other plants.

结果表明，转化的外源目的基因多数以嵌合形式，少数以单拷贝形式整合到转基因甘蓝型油菜的基因组中；目的基因能稳定地遗传给后代，株系间遗传传递率为62.5％；T_2代的遗传行为少数呈现孟德尔遗传，多数呈现非孟德尔遗传现象；拟南芥CRY1基因能在转基因油菜中表达，但也出现了沉默现象。

Circular RNA/DNA chimeric oligonucleotides to transfect cells and make a single?

本文介绍了RNA/DNA嵌合分子介导的高效基因修复技术。

AIM: To determine the cleavage activity of anti-transforming growth factor β1 hammerhead ribozymes which was inserted into U1 small nuclear RNA in cell-free system.

目的：研究抗转化生长因子β1 U1snRNA嵌合型锤头状核酶的细胞外切割活性。

The cell cultures were monitored by provirus DNA PCR, RT-PCR, reverse transcriptase activity assay and real-time RT-PCR, The results of RT activity and DNA PCR, RT-PCR were positive in the supernatant of cell cultures and viral particles were also clearly observed under electron microscope.

本试验基于代次毒分析结果，选取LTR 变异率较高的U3 区，以驴胎皮肤弱毒疫苗感染性分子克隆株pLGFD3-8 和驴强毒株感染性分子克隆株为父本操作系统进行基因替换，构建EIAV 非编码区强弱毒嵌合的全基因分子克隆，将阳性重组质粒命名为pLGFD9-12。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine, thewhole genomes of DLA-EIAV and EJAV L provirus were cloned and sequenced. Aninfectious molecular clone derived from DLA-ELAV was constructed and characterized.Three DLA-EIAV/ L chimeric viruses were constructed. Promoting efficacy of the longterminal repeat of the DLA-EIAV was charactenzed.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

To understand the infectivity by porcine endogenous retrovirus with porcine skin fibroblast cell in vitro and in vivo, porcine skin fibroblast cell established by our laboratory were co-cultured with neo/HEK293 cell for the infection of RERV in vitro, and were subcutaneously transplantated to SCID (severe combined immuno-deficiency) mice for the infection of PERV in vivo, laying the foundation for valuation of biologic safety of xenotrans-plantation. The event of neo/HEK293 cells infected by PERV occurred during co-culture of porcine skin fibroblast cells with neo/HEK293 cells, expanding the rang of the infection of porcine endogenous retrovirus. Afterpig cells transplantated subcutaneously in SCID mice, the microchimerism (78.57%) of pig cells occurred widel, and there was phenomena of integration of PERV provirus (85.71%) in several organs or tissues remote from the injected sites, indicating infection of PERV in SCID mice in vivo. yet, there is no evidence of active viral replication in analysis of PERV env RNA of these tissues or organs.

为了解猪皮肤成纤维细胞PERV在体外和体内的感染性，通过建立猪皮肤成纤维细胞系，将所建细胞系与人胚胎肾293细胞体外共培养，并移植于严重联合免疫缺陷鼠皮下进行猪皮肤成纤维细胞PERV的体外和体内感染性实验，结果表明，猪皮肤成纤维细胞与人胚胎肾细胞共培养过程中，猪内源性逆转录病霉感染人胚胎肾细胞，进一步证实和拓宽了猪细胞PERV感染人细胞的范畴；猪皮肤成纤维细胞移植SCID鼠皮下后，导致SCID鼠发生猪细胞微嵌合(78.57%)和PERV在体内感染(85.71%)并且波及远离移植部位的多种组织或器官，但是并未检测出SCID鼠组织中表达PERV env RNA。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine,thewhole genomes of DLA-EIAV and EIAV L provirus were cloned and sequenced.Aninfectious molecular clone derived from DLA-EIAV was constructed and characterized.Three DLA-EIAV/L chimeric viruses were constructed.Promoting efficacy of the longterminal repeatof the DLA-EIAV was characterized.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

The gene expression of chimeric virus could be detected in 293T cells transfected by SHIV proviral DNA.

用此 SHIV 原病毒 DNA 转染 293T 细胞，细胞中能够检测到嵌合病毒基因的转录与翻译；在细胞培养液上清中亦可检测到装配出的病毒颗粒。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失