嵌合

A TYLCCNV DNAβhybrid having TbCSV DNAββC1 lost the ability to elicit the vein thickening and enation phenotypes.On the other hand,TbCSV DNAβ hybrids containing TYLCCNV DNAβpC1 or the AP fragment failed to induce the characteristic vein thickening and enations.

嵌合有TbCSV DNAββC1的TYLCCNV DNAβ丧失了诱导脉增厚和耳突表型的能力；嵌合有TYLCCNV DNAββC1或AP片段的TbCSV DNAβ也不能诱导植株产生典型的脉增厚和耳突。

Type A band was detected in nearly all the organs and tissues,when coat color chimerism was over 85%, indicated the ES cellhas a fairly high potential to differentiate into cells of endoderm, mesoderm and ectoderm,In addition, only type A band was observed in the muscles of 6 mice which coat color chimerism is over 85%,the results indicated that these muscles were differentiated only from ES cells.

当毛色嵌合率大于85%时，几乎所有的器官组织都检测到A型条带，显示了ES细胞在发育形成内、中、外胚层的细胞方面具有很高的分化潜能。另外，在毛色嵌合率大于85%的其中的6只嵌合鼠的肌肉中，只观察到A型的条带，表明这些肌肉只单独来源于ES细胞。

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗；(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力；(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性；(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡；(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型，应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况，明确其靶向性；(6)将荷瘤裸鼠分3组进行放射免疫治疗，分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

However, mosaicism of both CGG repeatlength and methylation status exists in fragile X patients.

全突变经常是嵌合性的，甲基化现象也会嵌合发生。

In addition, 19 (50.0%) embryos were considered to be chromosomal mosaics due to diploid mosaicism and abnormal mosaicsm.

在≤4细胞期胚胎中，嵌合型胚胎占18.2%，但在5～8细胞期和≥9细胞期胚胎中，嵌合型胚胎的比例分别增至68.4%和50.0%。

Objective Using fluorescence in situ hybridization to analyze chromosome mosaicism in human preimplantation embryos and access the influence of mosaicism on in vitro fertilization-embryo transfer and preimplantation genetic diagnosis.

目的 应用荧光原位杂交技术对人类早期胚胎染色体的嵌合型进行初步分析，并探讨胚胎染色体嵌合型对常规体外受精-胚胎移植成功率以及植入前诊断的影响。

ObjectiveUsing fluorescence in situ hybridization to analyze chromosome mosaicism in human preimplantation embryos and access the influence of mosaicism on in vitro fertilization-embryo transfer and preimplantation genetic diagnosis.

目的应用荧光原位杂交技术对人类早期胚胎染色体的嵌合型进行初步分析，并探讨胚胎染色体嵌合型对常规体外受精-胚胎移植成功率以及植入前诊断的影响。

The results indicated that:The multi\|locus DNA fingerprint with a new synthesized probe\|JL\|02,has enough polymerism and good stability,and should be very useful to monitor the chimerism in different tissues of ES cell chimeric mouse and to check whether an ES cell line has the capacity to enter the germ line,especially when involving strains that can not be discerned with coat color or biochemical markers.

结果表明：采用新型的人工合成的寡核苷酸多聚体探针(JL-02探针)的多位点DNA指纹图谱，具有足够的多态性和很好的稳定性。适用于鉴定ES细胞在嵌合体小鼠各种组织器官中的嵌合情况，也适用于鉴定ES细胞是否具有种系嵌合能力，尤其适用于在嵌合体研究中，最适宜的品种组合具有相同的毛色或相同的酶型。

Then replate them onto feeder layer culture system (containing leukemia inhibitory factor) in order to passage and expand. Subsequently we identified the expanding cells by alkaline phosphatase staining, karyotype analysis and the formation of embryoid body which are typical characteristics of ESCs.

以分离培养的细胞作为供体细胞，近交系C57BL/6J为受体囊胚供者，采用囊胚注射法构建嵌合胚，体外恢复培养后移植进动情期子宫中，结果生出了黑白杂合的嵌合鼠。

After 6 to 7 days of culture, Eleven ES cell lines were selected and explanted. At last we obtained 4,3,4 and 3 ES cell lines separately. One of the B6C3F_1×B6D2F_1 ES cell lines was selected to future study its competent for germline transmission.We founded the ES cell line has normal karyotype on the chromosome analyse.In teratoma experiment by injected ESCs into C57 mices inguen, we gained teratoma contained cells come from all three embryonic germ layers.

选择一株具有高度杂合背景的B6C3F_1×B6D2F_1 ES细胞系进行核型分析、畸胎瘤实验后与ICR品系2n胚胎构建嵌合体并得到具有生殖系传递能力的嵌合体小鼠，证明此细胞系具有生殖系嵌合能力。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失