山柰酚

Objective: To estabilish an RP-HPLC method for the determination of kaempferol, naringenin and quercetin in Euonymus alatus Thunb.

目的：建立同时测定鬼箭羽中降糖活性成分山柰酚、柚皮素及槲皮素含量测定的方法。

Their structures were identified by physico-chemical properties and spectral analysis. RESULTS Seven compounds were isolated as kaempferol,quercetin,astragalin,isoquercitrin,kaempferol-3-O-β-D-glucuronic acid methyl ester,4-hydroxybenzonic acid,hentriacontane.

结果分离得到7个已知化合物，分别为山柰酚、槲皮素、山柰酚-3-O-β-D-吡喃葡萄糖苷、槲皮素-3-O-β-D-吡喃葡萄糖苷、山柰酚-3-O-β-D-吡喃葡萄糖醛酸甲酯、对羟基苯甲酸、三十一烷。

A high-performance liquid chromatographic method for the simultaneous separation and determination of aryltetralin lignans and flavonoids in Podophyllum emodi Wall.var. chinesis Sprague was developed. The separation was performed on a Kromasil-C 18 column by gradient elution with phosphate buffer-methanol (0-13min, 50∶50, 33min, 30∶70) as mobile phase at a flow-rate of 0.8 ml/min, with detection 270nm.

首次建立了桃儿七中苦鬼臼葡萄糖甙、4-去甲基鬼臼脂素、表鬼臼脂素、苦鬼臼脂素、苦鬼臼酯酮、鬼臼脂素、鬼臼酯酮、去氧鬼臼脂素、去氢鬼臼脂素等9种木脂素以及山柰酚、槲皮素等2种黄酮类组分同时分离测定的高效液相方法。

Results Eleven compounds were isolated and identified as:quercetin(1),kaempferol(2),quercitrin(3),protocatechuic acid(4),gallic acid(5),protocatechuic acid ethyl ester(6),ethyl gallate(7),vanillic acid(8),1,5,7-trihydroxyl-3methylan...

结果得到11个化合物，分别鉴定为：槲皮素(1)、山柰酚(2)、槲皮苷(3)、原儿茶酸(4)、没食子酸(5)、原儿茶酸乙酯(6)、没食子酸乙酯(7)、香草酸(8)、1，5，7-三羟基-3-甲基蒽醌(9)、琥珀酸(10)、5-羟甲基糠醛(11)。结论化合物6、7、9、11为首次从该属植物中分离得到。

Three flavonoids compounds: kaempferol *!, quercetin *, and isoquercitrin *# were isolated from the leaves of Cyclocarya paliurus Batal.

采用溶剂萃取及多种色谱技术分离纯化，根据理化性质和光谱分析方法鉴定其结构，确定从青钱柳中分离得到山柰酚、槲皮素和异槲皮苷 3个黄酮单体化合物，并用反相高效液相色谱法对其含量进行了测定，测得各自在甲醇和水提取物中的含量：山柰酚 0 。

RESULTS Kaempferol, quercetin and impurities were separated.

结果 槲皮素，山柰酚与相邻杂质能很好分离，其分离度分别为1.96和5.32，理论塔板数以槲皮素峰和山柰酚峰计算，分别为5121和6673；在5～100mg·L^-1内峰面积与其浓度均呈良好的线性关系（r=0.9994，0.9996），样品回收率分别为97.58％和105.67％；两成分含量以湖南张家界的最高而湖北赤壁最低。

Kae is effective in inhibiting the action of PAF and it is a new PAF receptor antagonist.

山柰酚具抗PAF的作用，为一新的PAF受体拮抗剂。

The calculated thermodynamic parameters suggested that the interaction between KAE and DNA was driven mainly by hydrophobic force.

由求得的热力学参数推断山柰酚与DNA结合的驱动力主要是疏水作用力。

Results showed that hypochromism and red shifted KAE absorption spectra were observed in the presence of DNA,and that synchronous fluorescence of DNA-NR was quenched with addition of KAE,indicating that a strong competition for DNA binding ...

结果表明：加入DNA后，山柰酚的吸收光谱呈现减色和红移现象；山柰酚的加入使得DNA-NR同步荧光强度猝灭，表明山柰酚对NR与DNA的结合为竞争性抑制；山柰酚的加入使得DNA的粘度增大，热变性温度升高。

Results showed that hypochromism and red, shifted KAE absorption spectra were observed in the presence of DNA, and that synchronous、 fluorescence of DNA-NR was quenched with addition of KAE, indicating that a strong competition for DNA binding between KAE and NR existed, in addition, the relative viscosity and the value of melting temperature of DNA increased in the presence of KAE.

结果表明：加入DNA后，山柰酚的吸收光谱呈现减色和红移现象；山柰酚的加入使得DNA-NR同步荧光强度猝灭，表明山票酚对NR与DNA的结合为竞争性抑制；山柰酚的加入使得DNA的粘度增大，热变性温度升高。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。