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When C.farreri×P.yessoensiswere tested by using6pairs of microsatellite primers which could be applied to boththe two kinds of scallops,the specific bands could be amplified fromeachone of the 6 pairs,among which,the specific bands fromboth agnate and maternal colony could be distinguished obviouslyinthe amplified products of P13F449 and KMY134,and accordinglythe hybrid identity of hybrid generations could be affirmed.

将这6对具有属间通用性的微卫星引物对栉孔扇贝×虾夷扇贝进行PCR扩增,均能扩增出特异性条带,其中引物P13F449和KMY134的扩增产物中可以明显地分辨出来自父母本群体的特有条带,从而确定了杂交子代的杂种身份。

With rye cultivar AR71 as the source of resistance to wheat sharp eyespot,we tried to transfer the rye resistant genes into eight wheat cultivars or lines by pollen tube pathwaymethod and intergeneric crossing between wheat and rye.

以抗小麦纹枯病的黑麦AR71为抗源供体,以鲁麦1号、有蜡、百农3217、圆锥小麦TG29等8个小麦品种为受体,试图通过花粉管通道法和属间杂交的方法,导入供体的抗纹枯病基因。

Based on the Liriodendron interspecies hybridization study by professor Ye Peizhong,according to the satisfying and exact mating system and field design,the reciprocal cross with extensive genetic background of Liriodendron tulipifera Linn., the backcross and F 1 crossing which had never been made, and the genetic analysis of heterosis of the hybrid tulip tree were carried out.

在叶培忠先生的鹅掌楸属种间杂交育种研究基础上按遗传设计与环境设计开展了扩大北美鹅掌楸遗传基础条件下的正反交以及前人从未做过的回交和F1 间杂交,并进行了杂种马褂木杂种优势的遗传分析。

Chicken and quail intergeneric hybridization is a classic distant hybridization in fowl. In fowl science, chicken and quail belong to Aves, Galliformes, Phasiamidae, Gallus and Coturnix, and this hybridization combination has become the example of researching the incompatibility of different germ cell and sterile of distant hybridization.

鸡与鹌鹑杂交是属间杂交,其杂交种胚胎早期出现大量的死亡,并且杂交种出壳以前雌性胚胎全部死亡,这使鸡与鹌鹑杂交成为研究异种生殖细胞不亲和性和杂交种不育的典型。

Orientale additional and substitutional lines. chromosome number of 96 BC2 F3 individuals were accounted. Fifteen plants with 2n= 43 and eight plants with 2n= 44 were checked out. GISH results of the individuals with 43 chromosomes showed that they blonged to three different monosomic additions. Two disomic additions, ine double-monosomic addition, one trible- monosomic addition and one monosomic addition were found in the 2n = 44 individuals. One double-monosomic substitution and one monosomic sustitution were obtained.

为了筛选小麦-旱麦草异附加系、异代换系,对普通小麦品种Fukuho×东方旱麦草属间杂种的BC2F3代材料的96粒种子进行了染色体数目的检测,共检出15粒2n=43的种子,8粒2n=44的种子,进一步对以上材料进行的基因组DNA原位杂交,共鉴定出3个单体附加系,2个二体附加系,1个双单体附加,1个小麦三体单体附加,1个附加3条东方旱麦草染色体的小麦单体,在染色体数为42的个体中,检测出1个单体代换,1个双单体代换。

Basing on our early stage research work about collection and description of sugarcane germplasm and the aided project of Yunnan Natural Science Fund (2001 C0015Q), the wild species Erianthus fulvus, which has good characters, cold-resistant, drought-resistant, higher Brix and less chromosome number, was utilized for the first time through intergeneric hybridization with sugarcane cultivar. Its F_1 hybrids were appraised with some new techniques and methods for germplasm enhancement.

本研究在课题组考察采集和保育鉴定甘蔗野生种质资源的前期工作基础上,依托云南省自然科学基金资助项目(2001C0015Q),首次利用具有特异优良性状(抗旱、耐寒、锤度高、染色体数目少)的蔗茅野生种与甘蔗栽培种或品种进行属间远缘杂交,扩大甘蔗遗传多样性;采用新技术、新方法对蔗茅杂种F_1代材料进行早期鉴定评价研究,筛选具有育种价值的新种质,为进一步选育生态适应性广、抗逆性强、丰产高糖的甘蔗新品种奠定物质基础。

Besides, there still have never been reported about the situation of wild species originated from China in evolution of strawberry, research on cytogenetics of hybrids from the cross of wild species originated from China with cultivars of Fragaria will also be helpful for understand the relationship between wild species and cultivars and strawberry origin and evolution.

另一方面,原产我国草莓资源在草莓属进化中的作用至今尚未见报道,通过种间杂交及细胞遗传学研究可有助于探明草莓种质间的亲缘关系,为了解草莓的起源和进化提供参考。

The results show that,pollen mentor is an effective method to overcome incompatibility between Prunus mume and some other Prunus spp.

结果表明,花粉蒙导是克服梅花与其它李属植物种间杂交不亲和性的有效方法。

Sativa× Shennong 133 was done. Traits of both purpled awn and purpled stigma were appearance in F1 and purpled plant was appearance in F5, respectively.

bicolor品种沈农133为父本,进行属间远缘杂交,F1出现双亲没有的紫色芒子、紫色柱头新性状,F5出现紫色单株。

Aimed at the arising problems such as the cross-incompatibility and hybrid's sterility during distant hybridization of fruit trees, effects of different treatments on cross-compatibility of distant hybridization in stone fruits were studied based on the former studies, then physiological and biochemical changes during hybridization between plum and apricot were primarily discussed, and rescue and culture system of hybrids' embryos were also conducted, further the genetic variation and principle between the hybrids and their parents were analyzed and methods of identification of hybrids were established.

针对果树远缘杂交过程中存在的杂交不亲和性及杂种不育性等问题,在前人研究的基础上,研究了不同处理对核果类果树远缘杂交亲和性的效应,并探讨了李、杏属间杂交过程中发生的生理生化变化以及花粉与柱头的互作,建立了李、杏属间远缘杂种的胚培养技术体系,建立了杂种鉴定检测方法,并分析了杂种后代与其父母本之间的遗传变异及规律,最终将一批远缘杂交新种质定植于大田,同时对自然界存在的杏梅种质资源进行了RAPD分析。

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