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The left and superior layers of the coronary ligament, the falciform ligament and the caudate lobe of liver are appeared simultaneously on any same transverse-sections from the second to the first hepatic portal.

从第二肝门至第一肝门平面的横断层上,可见冠状韧带上层与冠状韧带左层、镰状韧带、肝尾状叶总是同步出现。

The biomechanical tests showed that two kinds of artificial bones had not significant difference on compressive strength and Young\'s modulus(P>0.05),while the flexural strength of nano-nacre artificial bone was less than the control group(P<0.05).3.The results of CCK-8 showed that the difference were not significant in each group,the proliferation of osteoblast reached the peak at the 5th day;7 days after being co-cultured,the total protein content of study group was higher than control group and blank group(P<0.05),while the difference between control group and blank group was not significantP>0.05The difference of alkaline phosphatase activities among three groups was not significant(P>0.05The SEM view showed that osteoblast attached and grew well in two kinds of artificial bone.4.X-ray photography showed that two kinds of powder started to degrade in 2 weeks;this phenomenon became more appear in 4 weeks,nano-nacre powder degraded faster than micron-nacre powder,while the hole shadow was easy to be found;in 8 weeks,all the femoral holes recovered and returned to normal bone mineral density in all groups.Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone grew fastest around the bone defect area in study group,while most slowly in blank groupP<0.05 SEM(scanning electron microscope observation showed that nano-nacre powder degraded more quickly.The same result can be found through the demineralized sections morphometric analysis,and both of the composite artificial bones made from those two kinds of nacre powder had the good connection with the adjacent tissue in rats body without apparent inflammatory response.5.X-ray photography showed that rabbit\'s bone defects healed faster in study group since NNAB implanted than in control group since MNAB implanted.At 24 weeks after operation,bone density in radial defects had nearly accessed to the normal area,while lower in control group,and turned up nonunion in blank group;The checking of BMD showed that results in study group were higher than those in control group at 8,16 and 24 week(P<0.05), and the difference between the BMD values in study group at 24 week and those in blank group was not significant(P>0.05).The gross specimens showed satisfactory histocompatibility both in study group and in control group,with bone tissue growing from two sides into the center of implanted materials; Normal slices in HE stain and hard tissue grinding slices in Stevenel\'s blue/Van Geison\'s picro-fuchsin stain showed that the bone growth tendency was better in study group than that in control group,and the medullary cavity had been penetrated to the implanted materials in study group at 24 week;Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone in both groups grew fastest 8 weeks after surgery,while slow down at 16 week.

纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨分别与成骨细胞共培养后,其各时间点CCK-8法检测值与空白对照无显著差异(P>0.05),成骨细胞均在第5天达到增殖高峰期;培养7天后,实验组细胞蛋白含量高于对照组及空白组(P<0.05),后两者之间则无显著差异P>0.05碱性磷酸酶活性在三组间均无显著差异(P>0.05电镜下可见成骨细胞在两种人工骨上都有良好生长贴附能力。4.X-ray显示两种粉体在大鼠股骨骨洞植入第2周时都开始出现了降解,第4周时更为明显,纳米珍珠层粉较之微米珍珠层粉降解更快,而空白对照组骨洞阴影仍可见,至8周时,则所有组骨洞均己闭合修复,X-ray下已不可见原钻孔痕迹,恢复正常骨质密度;硬组织磨片四环素荧光双标记结果显示纳米珍珠层粉植入组较其余两组在骨缺损区周围新骨生长速度更快,空白组速度最慢P<0.05电镜观察及常规脱钙切片亦可见到纳米粉体降解较快;由以上两种原材料制得的纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨在大鼠体内均与周围组织结合良好,无明显炎症反应。5.X-ray显示纳米珍珠层/消旋聚乳酸复合人工骨植入兔桡骨缺损区后其骨愈合速度较对照组微米珍珠层/消旋聚乳酸复合人工骨植入的快,至植入术后24周,实验组骨缺损区接近正常骨密度,对照组骨缺损区密度较低,空白组则呈现骨不连状态;骨密度测量结果显示术后8周、16周、24周实验组的骨密度值高于对照组(P<0.05,24周实验组的骨密度值与术前所测得的正常值无显著性差异P>0.05动物取材大体所见均显示组织相容性良好,骨组织逐渐由植入材料两端向中央生长;常规切片HE染色及硬组织磨片Stevenel\'s blue/Van Geison\'s picro-fuchsin联合染色均可见实验组骨缺损区长势优于对照组,至术后24周,实验组骨髓腔与材料已呈相交通状;硬组织磨片荧光显微镜下观察,两组材料在术后8周处于骨生长最快速时期,16周时速度开始减慢,术后4、8、16周时实验组的新骨生长速度均较对照组的快

Methods Axial lamellate computed tomography scans of frontal drainage system were firstly performed with four layers spiral CT, and coronal, sagittal sections then reconstructed with AW 4.1 software on 65 adults ,both frontal sinusitis group (35 cases, 66 sides) and control group (30 cases. 59 sides). Related cells in frontal drainage system were studied and compared dynamically.

采用4层螺旋CT对65例成人,30例正常成人及35例额窦炎患者的额窦引流通道行水平位薄层扫描后用Advantage Windows 4.1(AW4.1)影像工作站进行冠状位、矢状位重建,动态分析和比较两组额窦引流通道内出现的相关气房。

The outer neuroblastic layer before it divided to inner and outer nuclear layer and outer complexiform layer and gonglion cell layer showed a temporal PCNA positive which was related to proliferation. The three cell layers had another PCNA positive when finished time changed from outer to inner after their morphologic development basically completed( outer nuclear layer:11~15 days,inner nuclear layer: 8~16 days, gonglion cell layer:16~21 days).

PCNA显示在外成神经细胞层分化为内、外核层和外丛状层之前,和节细胞各有一次和增殖相关的阳性表达。3个细胞层在形态学的分化基本完成后,又出现了一次结束时间是由外向内变化的阳性过程(外核层:第11天至15天:内核层:第8天至16天,神经节细胞层: 16天到21天,这一次阳性表达过程可见到细胞形态向成年形态的转化。

RESULTS: VIP widespread expression existed on photoreceptor-outer, inner nuclear layer, inner plexiform layer of retinal and choroids; weak expression existed on ganglion cell layer and nerve fiber layer.

结果: VIP的表达主要位于视网膜光感受器外节、内核层、内丛状层和脉络膜,在神经节细胞层和神经纤维层有较弱表达。

Results: 1 The analysis revealed that GAP-43 immunopositive neurons in rats were localized in all the layers of lateral geniculate nucleus and the layers II -VI of visual cortex area 17,and GAP-43 is stained by a ring and dot figure and brown-yellow colour in cytomembrane ; 2, In the critical period, gene expression concentration and number of GAP-43 immunopositive neurons, in both the lateral geniculate nucleus and the visual cortex area 17, received from the deprived eyes, are reduced.

结果:1、GAP-43在正常大鼠视觉系统表达主要见于外侧膝状体全层和视皮质17区Ⅱ—Ⅵ层神经元胞膜中,呈环状或点状棕黄色免疫阳性反应;2、在敏感期内剥夺眼对侧外侧膝状体和视皮质17区GAP-43表达的免疫阳性神经元染色变淡(P<0.05),且免疫阳性神经元数目减少(P<0.05)。

RESULTS:In BN rat retina,Cx43 was immunolocalized to internal limiting membrane,nerve fiber layer,ganglion cell layer and pigment epithelium.Cx43 appeared in the form of distinct puncta distributed on ganglion cell.

结果:正常视网膜内界膜,视神经纤维层以及神经节细胞层明显表达,神经节细胞表达的主要位置是细胞质和细胞膜,色素上皮细胞全层表达,内外颗粒层及内外层状层无表达。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果:(1)层粘连蛋白组处于G_2~M期细胞比例较对照组显著提高,Go~G_1期细胞比例显著下降,提示层粘连蛋白促进内皮细胞DNA合成,及细胞分裂增殖;光镜下,对照组细胞分布成团状,细胞密度较低,细胞间连接紧密,细胞内微丝结成网状,细胞核大小一致;与对照组相比,层粘连蛋白组细胞生长旺盛,细胞密度高,向周边伸出大量板状及丝状伪足,细胞内微丝增多、拉长、集结成束,伸入伪足中,细胞核形状大小不一致;(2)倒置显微镜观察及细胞生长曲线显示,各组细胞数目随时间增加而明显增多,各实验组较对照组增生显著,EGF和LN联合应用组各时间点细胞数目最高;实验组处于S期和G_2~M期细胞数目增加,Go~G_1期细胞数目减少;提示EGF、LN单独及联合应用均可促进细胞增殖,但尚不能认为二者有交互作用;(3)倒置显微镜下,组织培养的猫角膜内皮细胞排列成密集的单层,细胞间连接紧密;组织学观察发现,培养的猫角膜内皮细胞形成完整的内皮层,贴附于脱水基质的后弹力膜上,与正常的角膜内皮组织结构相似;扫描电镜下,组织培养的猫角膜内皮细胞间紧密镶嵌排列,可见某些细胞呈近似六边形排列,细胞大小不甚一致,胞核清晰。

The fan delta, sand beach, sand bars and turbidite fan in the TST of SⅡ and SⅢ sequences are the most favorable exploration targets because of favorable source - reservoir - seal assemblages. Since the high - stand system tract in SⅡ sequence has good source - reservoir assemblage and is covered by the regional seal of SⅢ sequence, the hydrocarbon accumulation in this area is controlled by the local seal rock. The HST of SⅡ sequence becomes the second favorable exploration target zone. Because the scales of the sand bodies of alluvial fan and braided river in low - stand system tract of SⅡ and SⅢ sequences are limited, the formation of a large hydrocarbon accumulation is difficult.

良好的生储盖配置使SⅡ层序、SⅢ层序水进体系域的扇三角洲、滩坝及浊积扇砂体成为本区最佳勘探目标层。SⅡ层序高位体系域生储条件配置好,又有SⅢ层序区域盖层的整体封盖,油气聚集受局部盖层直接控制,可成为较有利的勘探目的层。SⅡ层序、SⅢ层序低位体系域的冲积扇、辫状河砂体体积规模较小,难以形成大油气藏。

Correlation between the two observers\'gradings was evaluated using analysis of Spearman. and P.05 was thought significance statistically.2 for the second objective of the study, 25 patients underwent helical 0.5mm axial CT, then the helical data was reconstructed with 0.5 mm slice width /increment and with 1.0 mm slice width /increment respectively, 15 abnormal structures of temporal bone were evaluated by a observer using a modified Likert scale, with unknown of the methods of imaging.

检验标准采用P<0.05.2对25例耳病患者行0.5mm准直多层螺旋CT扫描,将获得的容积数据以0.5mm层厚层距和1.0mm层厚层距各行横断和冠状重建,由一位神经影像诊断医师按照改良Likert scale评分标准对两种图像独立盲法评分。

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