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Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.
针对本实验目的,我们设计了以下的实验步骤﹔(1)二次代谢物回收及检测样本处理:我们将发酵所得的培养上清液,利用疏水吸附性树酯Amberlite XAD-2吸附回收,并以甲醇溶离及真空减压浓缩脱水等方式处理,以取得提供抗氧化活性筛选之检测样本;(2)抗氧化活性检测及菌种筛选:以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用;经此筛选程序,我们选获了具有抗氧化物质高生产力的菌株,命名为AMBL-029C;(3)抗氧化物质的分离纯化:针对生产菌株的发酵回收处理液,以矽胶薄层色层分离法经物质层析纯化后,并以高效能液相层析法(High performance liquid chromatography;HPLC)分析物质可得一初级纯化物质,命名为AMBL-029C-TS;(4)抗氧化物质的物理化学性质分析:由酸碱转溶(acid-base fractionation)实验得知,此抗氧化物质属於中低极性的强酸性物质,对温度(37℃-100℃)及酸碱度(pH3.0-13.0)均表现出高稳定性;(5)在抗氧化机制探讨方面,我们针对数种不同的抗氧化机制进行探讨,即: 1。
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E. , active layer (0~30 cm), hypoactive layer (30~50 cm) and relatively stable layer(50~100 cm).
结果表明,土坡水分状况分为3层,0~20 cm为活跃层,30~50 cm为次活跃层,50~100 cm为稳定层。
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The results showed that the soil water profile in 0~100 cm could be divided into 3 layers,i.e.,active layer (0~30 cm),hypoactive layer(30~50 cm) and relatively stable layer(50~100 cm).
结果表明,土壤水分状况分为3层,0~20 cm为活跃层,30~50 cm为次活跃层,50~100 cm为稳定层。
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During thawing period, its soil water changes as season changes (the dynamic rule of soil water has the important relation of the season): from June to July the quality-moisture content of soil water gradually increases and reaches the highest, and from July to August it begins to decreases. The range of the quality-moisture content of the ground litter and humus layer is lower than the illuvial horizon whose quality-moisture content is gradually increasing in this thawing season.
在土壤冻土融化期,草类落叶松林土壤水分存在明显的季节性变化,土壤枯枝落叶层、腐殖质层质量含水量比淀积层质量含水量变化幅度大;6~7月份土壤含水量逐渐增加,达到最高;8月份以后土壤含水量开始降低;土壤淀积层质量含水量保持逐渐上升趋势;坡顶土壤质量含水量明显小于坡底土壤质量含水量。
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The results indicate that the different thermal expansions and elastoplastic properties and distributed randomness of various mineral particles composed of oil shale will lead to the nonuniformity of thermal field , deformation field and stress field in heating process , which makes the particles of oil shale deform incompatibly : and the release of product pyrolysis results in irreversible damages of oil shale material and structure . These are the main reasons for the thermal fracture of oil shale .
研究结果表明:组成油页岩各种矿物颗粒热膨胀和弹塑性性质的不同及其分布的随机性,导致油页岩加热过程中温度场,变形场和应力场的非均匀性,使得油页岩颗粒间变形不协调和油页岩热解产物释放引起油页岩材料与结构性能发生不可逆的劣化损伤,这是油页岩发生热破裂的主要原因;热传导方式加热油页岩,温度的传递比较缓慢,加热井和生产井的间距不宜过大,对油页岩储层性质存在差异时,注热井应选择较高热导率的区段;油页岩的层理对油页岩地下原位开发形成渗透带至关重要,油页岩达到热解温度会产生附加的膨胀力,首先在层理处开裂,应充分利用油页岩层理赋存条件改造其渗透性。
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The results indicate that the different thermal expansions and elastoplastic properties and distributed randomness of various mineral particles composed of oil shale will lead to the nonuniformity of thermal field, deformation field and stress field in heating process, which makes the particles of oil shale deform incompatibly: and the release of product pyrolysis results in irreversible damages of oil shale material and structure. These are the main reasons for the thermal fracture of oil shale. When oil shale is heated through thermal conduction, temperature transfers slowly, so the spacing between heating well and production well should not be kept too long. When difference exists in oil shale reservoir's properties, heating well should be chosen in the high conductivity region. Oil shale's stratification is vital for developing permeation zone in in-situ retorting. Oil shale will generate additional expansion force as it reaches its pyrolysis temperature, and stratification will be cracked first, so the oil shale stratification should be utilized to enhance its permeability.
研究结果表明:组成油页岩各种矿物颗粒热膨胀和弹塑性性质的不同及其分布的随机性,导致油页岩加热过程中温度场、变形场和应力场的非均匀性,使得油页岩颗粒间变形不协调和油页岩热解产物释放引起油页岩材料与结构性能发生不可逆的劣化损伤,这是油页岩发生热破裂的主要原因;热传导方式加热油页岩,温度的传递比较缓慢,加热井和生产井的间距不宜过大,对油页岩储层性质存在差异时,注热井应选择较高热导率的区段;油页岩的层理对油页岩地下原位开发形成渗透带至关重要,油页岩达到热解温度会产生附加的膨胀力,首先在层理处开裂,应充分利用油页岩层理赋存条件改造其渗透性。
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The stitching method to enhance interlaminar toughness of stitched-laminates was mainly discussed in this paper based on our research and experiment.
在缝合式层间强度的提高研究中,从断裂力学的角度,采用作为层间裂纹扩展阻力的能量释放率来评价层合板复合材料的层间断裂韧性。
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Further more, a conforming interpolater modeled with one-layer solid elements is proposed, obtaining the same simulated result comparing to the one with solid continuum elements when interpolater is thinner than 30mm.
在此基础上,提出连续模面单层实体单元包覆垫层等效模型,当弹性垫层厚度小于30mm时,它和连续模面多层实体单元包覆垫层等效模型有相同的模拟效果。
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The results show that when the cathode humidified temperature increase, the humidification of membrane and fuel cell performance improved at lower current density, while the saturation in diffusion layer increase and the performance decreases at higher current density. Humidification of membrane and fuel cell performance improved when the anode humidified temperature increase, the saturation in diffusion layer decrease and fuel cell performance improved when the fuel cell working temperature increase, the resistance of diffusion layer decrease and the performance improved when the porosity largen. At last, the simulation results are compared with the experimental results and they are basically the same.
结果表明随阴极加湿温度的提高,在低电流密度运行时膜的润湿条件改善,燃料电池性能提高,在高电流密度运行时扩散层中液态饱和度增加,燃料电池性能下降;随阳极加湿温度的提高,膜的润湿条件改善,燃料电池性能提高;随燃料电池运行温度的提高,扩散层中液态饱和度下降,燃料电池性能提高;随气体扩散层孔隙率增加,气体扩散层阻力减小,燃料电池性能提高。
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The results showed that the vertical structure of Lithocarpus glaber forest was rather complicated, and could be divided into arbors layer, shrub layer, herbaceous layer, and interstratum plants.
结果表明,石栎群落垂直结构较为复杂,可分为乔木层、灌木层、草本层,亦有少量的层间植物。
- 推荐网络例句
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I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""
客人们在卡罗利娜·埃凯家里,举止就文雅一些,因为卡罗利娜的母亲治家很严厉。
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Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.
由著名品牌 Herm è s 设计,这件高贵的制服是香港本土制造,是我们第一套不配帽子的制服。
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Do not 'inflate' your achievements and/or qualifications or skills .
不要 '夸大' 你的业绩或成果,条件或者技能。