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In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

In this paper, by using the method of geometrical optics and the theory of fiber optics mode, we analyzed difference characteristics of double-clad fiber andthe effect of all kinds of DCF's inner-cladding structures on1 the absorption efficiency of DCF and its laser.

因此在本论文里采用几何光学和纤维光学模式理论的方法分析了不同内包层结构的双包层光纤对光纤激光器性能的影响，建立理论分析模型，并设计了新型内包层结构的双包层光纤。

Results: All rats in experiment group devloped CAG at the end of 12 weeks with the following Pathological changes of CAG such as thin mucosa layer, thick muscle layer, decreased glandes, soakaging inflammation cells in microscope and with the time prolonging, the atrophy of gastric mucosa became more serious; In normal control group and normal feeding group, the IL-6 and IL-8 content in blood serum are the same at every time point; In experiment group, IL-6 became increased from 4wk and kept high level from 4wk to l2wk and had significant difference compared with normal controls.

结果：灌胃至12wk时出现胃粘膜层变薄，固有层腺体减少，固有层纤维结缔组织增生，粘膜肌层明显增生，慢性炎细胞浸润等萎缩性胃炎表现，随著热盐水灌胃时间的延长，32wk胃粘膜萎缩程度明显加重；正常对照组和正常餵养组大鼠各时间点血清IL-6，IL-8含量基本相同。

In the second layer,namely supercooled water layer,there are composed by ice crystal,snow,graupel,cloud droplet and raindrop.

云的第一层对第二层有播种作用，冰晶层对降水的贡献为7%，过冷水层对降水的贡献为54%，暖水层对降水的贡献为39%，降水的产生中冷云过程作用稍大，但暖云过程也起重要作用。

It is the spin of the molecules along each lay line of grid work that pushes some of the chi and new information from the regeneration chakras down the lay lines of the grid work.

正是分子及晶格层每一条层线的旋转，将来自再生脉轮的一些气和新信息推动到晶格层的层线之中。

Based on experience and harvest obtained home and abroad, pavement structure of asphalt overlay is studied out by forecasting future traffic capacity. Mid-layer of overlay structure is the most adverse layer in the whole structure by mechanics analysis, so the material composition design of mid-layer is considered as emphasis.

借鉴国内外已有经验成果的基础上，通过对旧路面状况调查，结合未来交通发展的需求，进行了加铺层的结构组成设计，并从力学角度分析了中面层为加铺层结构的最不利层，因此对其材料组成设计进行重点研究。

A new thought line of reservoir characterization is put forward based on the combination of the high resolution sequence stratigraphy,the architectural element and flow unit analysis method.It integrates the static geological body with the dynamic developing process,the reservoir heterogenesis with oil and water distribution from macro scale to micro scale.

应用高分辨率层序地层、结构单元及流动单元分析的三位一体理论指导储集层的表征建模，从而形成由宏观到微观、静态地质实体与动态开发过程、储集层非均质性与油水分布及流动规律相结合的体系是储集层精细描述的新思路。

The application of the permeability obtaining with stoneley-wave inversion in those complex lithology reservoirs with lower porosity and permeability shows that the permeability from stoneley-wave is a good reference to divide into zone for those reservoirs with relatively good wellbore conditions, big sediment thickness and good homogeneousness. This enlarges the application scope of acoustic logging.

斯通利波渗透率在低孔、低渗透性复杂岩性储层的应用结果表明，对于井眼条件相对较好、储层发育厚度大且均质的该类储层，可参考斯通利波渗透率进行储层的有效性划分，从而拓宽了声波测井的应用范围。

The fixed-NH+4 capacity of all natural layers in lou soil was determined in this thesis,they were 482.2±10.4,1134.9±4.0 and 553.0±11.3 mg N／kg soil in overburden layer,claying horizen and loess parent material horizen respectively.

确定了土娄土各自然层的固定态铵容量，覆盖层、粘化层和黄土母质层分别为(482.2±10.4) mgN/kg、(1134.9±4.0) mgN/kg和(553.0±11.3) mgN/kg土。

Hornbeam's bough divides into three, first is a black, second is the brown, shinbone three is a yellow.

铁树的树干分为三层，第一层是黑色，第二层是棕色，骶三层是黄色。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。