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尿酸酶

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The three electrode system which composed of two platinum electrodes and saturated calomel electrode, act as working electrode, counter electrod...

当体液中的尿酸通过酶膜时,与酶进行反应,检测出该处产生的反应电流,然后将其换算成尿酸浓度加以显示。

The last part of this article emphasis on the mechanism of cichory extract N2. Hepatic lipase, NO and xanthine oxidase are observed in this aspect, and gene expression of hepatic lipase is also detected.

本部分研究观察了菊苣提取物 N2 对影响脂质代谢的肝脂酶、尿酸代谢的关键酶黄嘌呤氧化酶、一氧化氮的活性和含量,还从分子生物学角度观察了肝脂酶的基因表达。

Methods: Research on middle-aged and old person who hospitalized in our hospital from January, 2000 to October,2006, measure the blood uric acidwith zymology, the boundary is SUA which is bigger than 420mmol/l, Comparate and analysis the clinical material of hypeluricemia group, hypeluricemia merge coronary disease group and normal uric acid group.

以2000年1月至2006年10月在我院住院的中老年人为研究对象,采用酶学测定血尿酸,以SUA大于420mmol/l为界限,比较分析高尿酸血症组及高尿酸血症合并冠心病组与尿酸正常组的临床资料。

The experiment two: enzyme preparation significantly improved average daily gainand feed conversion ratio (P<0.05). Enzyme preparation significantly increased energymetabolizability and digestibility of crude fiber, crude protein and neutral detergent fiber,but had no remarkable effect on digestibility of dry matter, crude fat and acid detergentfiber. Enzyme preparation significantly decreased the relative viscosity of duodenal andjejunal digesta. The pH of intestine had no noticed difference in all groups. Enzymepreparation significantly decreased relative weight of gizzard, proventficulus, duodenum,jejunum and ileum. Enzyme preparation significantly increased villus size of duodenumand jejunum, and villus to crypt ratio of duodenum and ileum significantly increased too.Enzyme preparation considerably decreased ileal crypt height (P<0.05), and didn"t affectthickness of intestinal wall. Supplementing enzyme preparation, the serum glucose, totalprotein and alanine aminotransferase, but enzyme preparation hadn"t noticed influenceupon uric acid, total cholesterol, triglyceride and high-density lipoproteins. Enzymepreparation significantly increased insulin, triiodothyronine and insulin-like growthfactor-Ⅰ. Adding enzyme preparation, the percentage of thyroid stimulating hormone andgrowth hormone in the serum increased 16.44%, 19.18% and 18.84%, 21.74%respectively, and the percentage of glucagon and thyroxine decreased 12.07%, 14.36% and 13.79%, 15.40%, but failed to reach statistical significance (P>0.05). Enzymepreparation significantly increased (P<0.05) the trypsin and amylase activity of duodenaland jejunal digesta, but enzyme preparation didnt affect significantly (P>0.05) theintestinal lipase activity and pancreatic digestive enzyme. Enzyme preparation had nosignificant effect on caecal microbial population.

试验二:酶制剂显著提高平均日增重和饲料转化率(P<0.05);酶制剂显著提高能量代谢率及粗纤维、粗蛋白、中性洗涤纤维消化率(P<0.05),而对干物质、粗脂肪、酸性洗涤纤维消化率影响不显著;酶制剂显著降低十二指肠和空肠食糜相对粘度(P<0.05);添加酶制剂对肠道pH影响不显著;酶制剂显著降低肌胃、腺胃、十二指肠、空肠、回肠相对重(P<0.05),显著提高十二指肠和空肠绒毛高度,显著增加十二指肠和回肠绒毛高度/隐窝深度,降低回肠隐窝深度(P<0.05),对肠壁厚度影响不显著;酶制剂显著提高血清葡萄糖、总蛋白和谷丙转氨酶浓度(P<0.05),对尿酸、总胆固醇、甘油三酯及高密度脂蛋白浓度影响不显著,显著提高胰岛素、T_3、IGF-Ⅰ水平,添加酶制剂后,促甲状腺激素、生长激素分别提高16.44%、19.18%和18.84%、21.74%,胰高血糖素和T_4分别降低12.07%、14.36%和13.79%、15.40%,但差异不显著;酶制剂对胰腺消化酶活性影响不显著,显著增加十二指肠和空肠胰蛋白酶、淀粉酶活性,对小肠脂肪酶活性影响不显著;酶制剂对盲肠微生物菌落数影响不显著。

Laboratory evaluation consists of hematocrit (hemoconcentration suggests preeclampsia), platelet count, protein excretion, serum creatinine, serum uric acid, serum alanine and aspartate aminotransferase concentrations, and lactic acid dehydrogenase concentration.

实验室评估包括红细胞压积,血小板计数,蛋白排泄,血肌酐,血尿酸,血清丙氨酸氨基转化酶和天门冬氨酸氨基转化酶,和乳酸脱氢酶浓度

The level of serum uric acid and urinary uric acid were determined by phosphotungstic acid method.

目的 观察青秦液对高尿酸血症大鼠尿酸代谢及相关酶活性的影响。

Methods Acteoside was extracted from roots of Scrophularia ningpoensis .

从玄参根中分离提取苯丙素苷成分Acteoslde,采用矿黄嘌呤造成小鼠高尿酸血症,观察Acteoside对小鼠高尿酸血症的影响,并进一步从体外观察Acteoside对黄嘌呤氧化酶的冲制作用。

Objective To study the content of uric acid in serum, to screen the uricemia patients, and to avoid the diseases caused by uricemia. Methods An enzymetic colorimetric method was used to determine uric acid of 2 525 middle school students in Shanghai.

目的 了解中学生血清尿酸含量,以便尽早发现高尿酸血症患者,避免由此引起的疾病方法采用酶比色法,应用日立-7150型全自动生化分析仪上对上海市2 525名中学生的空腹血清进行尿酸测定。

This work is based upon the characteristic of an enzyme capable to recognize a particular molecule through an oxidation-reduction effect. In this study, the uricase is utilized as the sensing components of our sensor system to initiate biological and subsequent electrochemical reactions. After the reactions, the output infinitesimal currents are transformed into voltages shown as electrical signals.

本研究是利用酵素能够藉由氧化还原作用以识别特定分子的基本特性,使用尿酸酶做为感测器系统之生物电化学反应的感测元件,再将输出的微小电流转换为电压并呈现一电讯号。

According to the dope and de-dope principle of conducting polymers, the active enzymes (for example glucose oxydase, uricase, and so on) were reimmobilized into the conducting polymer film, and formed the good performance conducting polymer-enzyme molecule assembly.

利用导电聚合物的掺杂去掺杂原理,将活性酶分子(如葡萄糖氧化酶、尿酸氧化酶等)重新固定到该导电聚合物膜中,形成性能良好的导电聚合物-酶分子组合体。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

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