尿素酶

Results: VTTEK ANI, the anaerobic bacilli detecting card, showed that Bartonella strains were negative to oxidase, catalase, PO4, PHC,βGAL, aGAL,βGLU, aGLU, URE, GLU, and positive to Amino acid but PRO. The Rapid TD32 A system revealed that all strains reacted negatively to URE,αGAL,βGAL,βGP,αGLU,βGLU, RAF, MNE.

结果：VITEK ANI厌氧菌鉴定卡检测结果：氧化酶、过氧化氢酶、半乳糖苷酶、葡萄糖苷酶等反应阴性，能够分解多种氨基酸，但不能分解脯氨酸，不能利用尿素、葡萄糖等糖类。

Methods:A rabbit model was developed with scald complicated with artificial infection of Pseudomonas aeruginosa.Studies were carried out by using the combat trauma spray group and the solution group.At day1,2,4,7,14,post experiments,rabbit serum was collected,the body temperatures of animals were taken,and such inflammatory factors as white cell count,serum levels of NO 2-/NO 3-,serum levels of tumor necrosis factor,serum superoxide anion,as well as GPT,AKP,aspartate carbamyl transferase,urea nitrogen were also collected to measure the functions of both the kidney and liver.

采用烫伤合并人工感染绿脓杆菌的家兔模型，用战伤喷剂与溶剂组于实验前和实验后第1、2、4、7、14天采集家兔血清，测量体温，检测白细胞数、血清NO 2-/NO 3-水平、血清肿瘤坏死因子水平、血清超氧阴离子等炎性因子水平以及谷丙转氨酶、碱性磷酸酶、天冬氨酸转移酶、肌苷和尿素氮等肝肾功能指标水平。

It was investigated that the immobilized catalase was affected by temperature and pH and inhibited by hydrogen peroxide.

研究了温度和pH值对酶活性的影响及过氧化氢和尿素对固定化酶的抑制作用。

Under the optimized conditions as follows: ratio of apple pomace and cottonseed meal 1︰1, urea content 2.6%, KH2PO4 content 0.09%, glucose content 2%, initial moisture content 62.9%, fermented at 30℃ for 60 h, 5662 and 30000 U/g of xylanase and cellulase were produced, respectively.

在最佳条件（基料棉粕和苹果渣比例为1︰1，第2氮源尿素2.6%，无机盐KH2PO4 0.09%，速效碳源葡萄糖2%，含水率62.9%，30℃培养60 h）下，分别获得了5662、30000 U/g的木聚糖酶和纤维素酶的高酶活发酵干曲。

They were reversible un-competitive inhibitors, and their inhibition constants of free enzymeK(subscript I were 254.00 and 136.78 mmolL^(-1), respectively. Urea was reversible mixed-type inhibitor, and its inhibition constants of free enzymeK(subscript I and enzyme-substrate complexK(subscript IS were determined to be 379.02 and 1182.00 mmolL^(-1), respectively.

Na2SO3和H2O2对酶的抑制均表现为可逆反竞争性类型，它们对游离酶的抑制常数K(下标 I分别为254.00和136.78 mmolL^(-1)；尿素对酶的抑制表现为可逆的混合型抑制，它对游离酶的K和对酶－底物复合物的抑制常数K(下标 IS分别为379.02和1182.00 mmolL^(-1)。

Otherwise, the application of chemical fertilizers enhance the activities of saccharase and phosphatase, as while, to enhance its root vigors in the appalication of organic and conventional fertilizer. The activities of urease was the highest at the treatment with 72000 kg/hm2 mature of human (296.8 mgNH3-N/g DW), the activities of proteinase was the highest at the treatment with 36000 kg/hm2 mature of duck (5.15 mg NH2-N/g DW), the activities of cellulase was the highest at the treatment with 36000 kg/hm2 pig mature (0.869 mg glucose/g DW), the activities of saccharase and phosphatase were both the highest at the treatment with 1980 kg urea, 3600 kg/hm2 Ca3(PO4) and 1305 kg/hm2 K2SO4, having the value of 13.10 mgglucose/g DW and 44.46 mg P2O5/100g DW respectively.

土壤脲酶活性以施用72000 kg/hm2人粪尿的处理最高（296.8 mgNH3-N/g DW），蛋白酶以施用鸭粪36000 kg/hm2的处理最高（5.15 mg NH2-N/g DW），纤维素酶以猪粪36000 kg/hm2的处理最高（0.869 mg葡萄糖/g DW），蔗糖酶、磷酸酶活性均以施用1320 kg/hm2尿素、2400 kg/hm2 Ca3(PO4)和870 kg/hm2K2SO4的处理最高，分别达到13.10 mg葡萄糖/g DW和44.46 mg P2O5/100g DW。

Potassium, natrium determines it is good that use ion chooses electrode indirect method it is better also with indirect method to determine at calcium of direct way;, it is next occasionally phosphor of; of law of azotic arsenic Ⅲ determines use phosphor molybdic acid is ultraviolet law outcome is better it is better with result of law of personal candy kinase that; blood sugar determines; urea determines use Niao enzymatic ultraviolet anhydride of better; flesh uses rate law result enzymatic law outcome is better; albumin determines, it is vanadium acid next alkalescent phosphoric acid of oxidation law; is enzymatic determine outcome of fluid of use AMP amortize is better; amylase determines law of thing of enzymatic standard background and iodic colorimetric law coefficient of variation all bigger, former a bit small, latter of percent of pass is tallish, each lab can try to choose according to his condition.

结论钾、钠测定使用离子选择电极间接法好于直接法；钙测定用间接法也较好，其次是偶氮砷Ⅲ法；磷测定使用磷钼酸紫外法结果较好；血糖测定用己糖激酶法结果较好；尿素测定用脲酶紫外速率法结果较好；肌酐用酶法结果较好；白蛋白测定用溴甲酚绿法较好；胆红素测定用酶法结果好，其次是钒酸氧化法；碱性磷酸酶测定使用AMP缓冲液结果较好；淀粉酶测定酶法底物法和碘比色法变异系数均较大，前者稍小，合格率后者稍高，各实验室可根据自己的条件加以选用。

In this thesis, the submerged fermentation and solid-state fermentation of Coriolus versicolor laccase, the enzymatic characterization, and the application of laccase in indigo and gold orange Ⅱ decolorization was investigated. The major results were as follows:The laccase production by Coriolus versicolor was performed in submerged fermentation, using corn husk as carbon source and carbamide, ammonium sulfate as nitrogen source.

本文对Coriolus versicolor漆酶的液体发酵和固态发酵进行了研究，并且对Coriolusversicolor漆酶的酶学性质及其在靛蓝、金橙Ⅱ脱色降解方面的应用进行了探讨，其研究成果如下：对Coriolus versicolor漆酶的液体发酵工艺参数进行了优化，结果表明，以玉米皮为碳源，尿素和硫酸铵为氮源，利用Coriolus versicolor液体发酵产漆酶时，C／N以20为宜。

The results indicated that the optimum OBEOP condition was showed as enzyme OX-1 of 4.5 g/L, 30% H2O2 of 15 g/L, temperature of 95 ℃, processing time of 40 min, pH value of 10~0.5, bath ratio of 1:30. It is found that the whiteness, woolliness and the strength loss with enzyme was superior to those treated with traditional sodium silicate process. Adding urea in OBEOP can help to improve the fabric whiteness, woolliness and reduce the strength loss.

试验结果表明：棉织物酶氧一浴最佳工艺条件为：净棉酶4.5g/L，双氧水12mL/L，温度95℃，处理时间60min，pH值10~10.5，浴比1：30；经净棉酶处理后棉织物的白度和毛效优于碱氧退煮漂一浴法工艺，而且织物的强力损失少；在棉织物酶氧一浴法工艺中添加尿素，有助于提高织物白度、毛效，并能减小强力的损失。

The results showed that the activities of urease, nitrate reductase, nitrite reductases and the yield of above ground plants were the highest with the fertilization pattern of taking 50 percent nitrogen from organic fertilizer and the other 50 percent nitrogen from urea.

结果表明：采用50%的N由有机肥提供，50%的N由尿素提供的施肥模式时，黑麦草根际土壤脲酶、硝酸还原酶、亚硝酸还原酶活性最强，黑麦草地上部产量最高。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。