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尿素酶

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Under conditions of 35 ℃ and pH=7, the activity of urease absorbed on TiO2 particles was measured by potentiometry.

在35 ℃,pH为7的条件下采用电位法测定吸附在TiO2上的尿素酶的活性变化。

The activity of urease is sensitive to bronopol.

尿素酶的活性对溴硝醇比较敏感。

Objective To develop and evaluate the method for determination of specific antibody against Helicobacter pylori Urease subunit B.

目的建立并评价幽门螺杆菌尿素酶B亚单位特异性抗体的检测方法。

The modified culture medium is fit to observe masculine fungi of urease and can reduce the single-valve experiment.

改进后的培养基易于尿素酶阳性菌的观察,可以减少单管试验。

Three gastric biopsies were taken within 2-3cm around the pylorus of gastric antra,urease detection,HE fuchsin staining and immunohistochemical analysis of Helicobacter pylori IgG antigen,HBsAg and HBcAg were performed separately.

2例肝病或非肝病因伴有胃病行胃镜检查的患者,取胃窦小弯距幽门周围( 2 - 3 )cm处粘膜 3块,分别做快速尿素酶试验及嗜伊红、品红染色,并行免疫组化检测幽门螺旋杆菌HPlgG型抗原。

All the isolates were first identified by different culture media including Niger seed agar. CACA agar, urease tests, ID32 identification kit, 37℃ growth test, and capability of forming capsule, and then the sequence of the LSUrDNA D1/D2 region were further analyzed.

分别以油菊籽、咖啡酸玉米琼脂、刀豆氨酸-甜菜碱-溴麝香草酚蓝培养基、尿素酶试验、37℃生长试验、ID32生化鉴定板和英膜形成试验等初步鉴定后,再经大亚基核糖体DNA的D1/D2区域序列分析进一步鉴定菌种。

The results Showed that twenty isolates have the following biological aspects:① Capability of forming capsule,② Growth at 37℃ within 3days,③ Forming brown colonies on Niger seed agar, CACA agar,④ Urea hydrolysis test positive within 96 hours.⑤ No blue colonies were found on CGB agar.⑥ The genus could be identified by ID32 identification kid, but the species could not.⑦ The sequence of the LSUrDNA D1/D2 region analyzed confirmed to be C. neoformans var neoformans.⑧ Serotype A and AD were found in these isolates, Serotype A was predominant(83.33%),⑨ Some biodiversities of isolates existed.

结果 20株菌皆具有以下特征:①形成英膜,②37℃3天内生长试验皆为阳性,③咖啡酸玉米琼脂和油菊籽培养基皆可以产生褐色菌落,④尿素酶试验96h皆呈阳性,⑤CGB培养基未见蓝色菌落生长,⑥ID32鉴定板可以鉴定到属,种的鉴定结果各不相同,⑦大亚基核糖体DNA的D1/D2区域序列分析证实皆为新生隐球菌的新生变种,⑧血清型仅见A和AD二型,以A型为多数(83.33%),⑨20株临床分离株随机扩增DNA多态性分析有一定的生态多样性。

Fasting and postprandial serum gastrin levels were determined by radioimm-unoassay in each 30 cases of normal gastroduodenal mucosa, chronic active gastroduodenitis, and active duodenal ulcer.

活检证实的正常胃、十二指肠粘膜,慢性活动性胃、十二指肠炎和活动性十二指肠溃疡各30例。胃窦部活检标本幽门螺杆菌尿素酶试验阳性者分别为16例,30例和30例。

Methods Rapid urease test was used to detect Hp present infection in26075cases examined by using the fiber gastroscope.

应用快速尿素酶试验对26075例接受纤维胃镜检查的患者作胃黏膜Hp现症感染检测。

Methods: 90 patients with upper digestive tract discomfort were endoscopied and the status of H-pylori was detected by histodiagnosis, mucous smear staining and rapid urease test, as well as by 14C-UBT.

对90例因上消化道不适而进行胃镜检查的患者,于胃窦部取多块活检标本进行组织学、粘膜涂片染色和快速尿素酶试验,以决定是否感染HP,并作14C-UBT。

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