尿素酶

"When urea is added to soils it is converted rapidly to ammonium carbonate by hydrolysis, a reaction which is activated by enzyme urease."

尿素施人土壤后很快水解成碳酸铵。这是由尿素酶催化的一种化学反应。

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

13 C-urea breath test（ 13 C-UBT）,histology and rapid urase test were used in diagnosing helicobacter pylori infection in 65 patients with gastric and duodenal ulcer,and 36 nomal persons.Any two tests of the above three were positive,the HP fection was confirmed.

对65例胃、十二指肠疾病患者和36例健康人行 13 C-尿素呼气试验、病理组织学和快速尿素酶三种方法检测幽门螺杆菌，其中任两项阳性者认为有HP感染。

40 patients presenting gastric symptoms underwent gastric mucosa biopsy while doing gastroscopy. All samples were analyzed by Wartin-Stany dying, rapid urase test, and 13C-urea breath test respectively as well as by histological examination. The patients with the same results by WS and RUT were regarded as chronic gastritis. In this patient, we analyzed the specificity, sensitivity and accuracy of 13CUBT.

取40例因上腹部症状而行胃镜检查的患者胃窦粘膜活检，分别行Wartin-Stany银染色、快速尿素酶实验、13C尿素呼气试验（13CUBT），并同时行病理组织学诊断；将WS和RUT结果相一致且病理结果为慢性胃炎的病例入选，分析13C尿素呼气试验（13CUBT）诊断慢性胃炎幽门螺杆菌感染的特异性、敏感性、准确性。

40 patients presenting gastric symptoms underwent gastric mucosa biopsy while doing gastroscopy. All samples were analyzed by Wartin-Stany dying, rapid urase test, and 13C-urea breath test respectively as well as by histological examination. The patients with the same results by WS and RUT were regarded as chronic gastritis.

取40例因上腹部症状而行胃镜检查的患者胃窦粘膜活检，分别行Wartin-Stany银染色、快速尿素酶实验、13C尿素呼气试验（13CUBT），并同时行病理组织学诊断；将WS和RUT结果相一致且病理结果为慢性胃炎的病例入选，分析 13C尿素呼气试验（13CUBT）诊断慢性胃炎幽门螺杆菌感染的特异性、敏感性、准确性。

All samples were analyzed by Wartin-Stany dying, rapid urase test, and 13C-urea breath test respectively as well as by histological examination. The patients with the same results by WS and RUT were regarded as chronic gastritis. In this patient, we analyzed the specificity, sensitivity and accuracy of 13CUBT.

取40例因上腹部症状而行胃镜检查的患者胃窦粘膜活检，分别行Wartin-Stany银染色、快速尿素酶实验、13C尿素呼气试验（13CUBT），并同时行病理组织学诊断；将WS和RUT结果相一致且病理结果为慢性胃炎的病例入选，分析13C尿素呼气试验（13CUBT）诊断慢性胃炎幽门螺杆菌感染的特异性、敏感性、准确性。

The tests often used were: rapid urease test 60%, urea breath test 40%, histopathology 36%.

常用的检测方法是：快速尿素酶试验60%，尿素呼气试验40%，组织病理学检查36%。

Results:thirty-nine citrobacters have urease genes,but only five have urease expression positive result.

结果：39株实验枸橼酸杆菌的尿素酶基因均为阳性，只有5株菌株尿素酶表达为阳性。

Stop drug 4 week hind check enzymatic experiment of gastroscope, fast urea and 14C urea are expiratory experiment, evaluate Hp eradicative rate and cankerous heal rate.

停药4周后复查胃镜、快速尿素酶试验和14C尿素呼气试验，评估Hp根除率及溃疡愈合率。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。