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AIM: To study the method for automatic analysis of blood vessels in digital chick embryo chorioallantoic membrane images and develop a special experimental analysis system based on digital image processing and biomedical engineering.

目的:以生物医学工程的角度,研究基于数字图像处理技术的鸡胚绒毛尿囊膜图像血管自动分析方法,设计和研制专用实验检测系统。

Methods RT-PCR, Western blot were used to detect the expression of bax in human gastric cancer cells line SGC-7901, and observe the effect of rhIL-24 on chorioallantoic membrane.

采用RT-PCR法和Western blot法检测rhIL-24对胃癌细胞株SGC-7901中促凋亡因子bax表达的影响,鸡胚绒毛尿囊膜技术观察其对血管形成的影响。

Objective: To study the angiogenesis promoting effect of Sexiang baoxin Pill on chicken embryo chorioallantoic membrane and cultured microvascular endothelial cells.

目的:探讨麝香保心丸在鸡胚绒毛尿囊膜模型及培养的微血管内皮细胞系统中的促血管生成作用。

We also found an angiostatin-like protein and the detection of its activity on chick embryo chorioallantoic membrane model shows a sign of inhibition of the positive effect of lung cancer cell culture supernatant on CAM.

对angiostatin类蛋白的功能鉴定结果发现在一定程度上可以抑制A459肺癌细胞培养上清对鸡胚尿囊膜血管生长的促进作用。

The molybdoneodymium heteropoly compound K_(11)[YPMo_(11O_(39)_2] was synthesized and used as catalyst in the synthesis of glyoxal by hydrogen peroxide and condensation of urea from allantoin .

尿囊素是一种被广泛应用于医药、农业和轻化工领域的重要的精细化工产品。

Carbamide reacting with H2SO4 forms aminosulfonic acid. Allantoin is prepared by oxidation of the aqueous solution of glyoxal into glyoxylic acid which is then used to synthesize allantoin.

氨基磺酸的合成是用尿素和发烟硫酸反应;尿囊素的合成是用硝酸氧化乙二醛溶液制得乙醛酸后,再加入氢氧化钙和氯化钙,使乙醛酸以钙盐形式析出,然后在盐酸介质中与尿素缩合而成。

The optimum work concentration of the indirect ELISA for detecting and screening positive hybridoma cells was established by research again and again. Polystyrene microtitration plates were coated with the purified DHV antigen at the amount of 0.5μg/well.

对鸡胚和鸭胚增殖的尿囊液病毒用反复冻融、氯仿处理、0.22μm滤膜过滤、PEG反透析浓缩以及Sephadex G-200分子筛层析的方法进行纯化,并以此来包被酶标板以及进行BALB/c小鼠免疫,效果良好。

Recombinant endostatin is a specific inhibitor of chick chorioallantoic membrane angiogenesis and human vitelline vein endothelial cell ECV-304 proliferation. The recombinant endostatin regressed established syngeneic B16 melanoma tumor in mice.

重组内皮抑素能够抑制鸡胚尿囊膜血管生成和人脐静脉内皮细胞ECV-304的增殖,在小鼠体内实验中对黑色素瘤产生良好的抑瘤效果(P<0.01)。

Methods Fifteen healthy, female SD rats were employed in the experiment. Sensitized systematically with keyhole limpet hemocyanin, the animals were inoculated with the same antigen through cochlea basal turn into the labyrinth. Adminstrated 5-bromo-2′-deoxyuridine intraperitoneally, the rats were sacrificed and the temporal bones were harvested at 3, 7,14 day after labyrinth vaccination respectively. The frozen sections of the decalcified samples were dealt with H-E staining and immunohistohemical methods to investigate the cellular infiltration, BrdUrd and IgG positive cells in the ES.

选用SD大鼠15只,以钥孔虫戚血蓝蛋白(keyhole limpet hemocyanin,KLH)全身免疫后,经耳蜗底回钻孔以相同抗原进行内耳免疫,然后分别在内耳免疫后3、7和14 d腹腔注射溴脱氧尿嘧啶核苷(bromodeoxyuridine,BrdUrd)后处死动物,取颞骨经组织学处理制作冰冻切片,用免疫组织化学技术观察内淋巴囊的细胞浸润、增殖和IgG细胞的分布状况。

OBJECTIVE To develop a method for the determination of allantion and lincomycin in Compound Lincomycin Hydrochloride Gel.

目的建立复方盐酸林可霉素凝胶中尿囊素和林可霉素的的含量测定方法。

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