尿囊

Methods Viruses were passed in embryonated hen eggs, and virion RNA was extracted from allantoic fluid and reverse transcribed to synthesize cDNA. cDNA was amplified by PCR and the PCR product was purified with a purification kit.

方法病毒在鸡胚中传代，从收获的尿囊液中提取病毒粒RNA，通过逆转录合成cDNA.cDNA通过PCR进行扩增，接着PCR产物用纯化试剂盒进行纯化。

The GPMV-containing allantoic fluid sample was added to the membrane and allowed to react with 3A5-coated particles.

该试纸条不与AIV、EDSV、IBDV、IBV、GPV、CELO的尿囊液和MV、CDV发生交叉反应，说明该试纸条有较高的特异性。

The indirect ELISA developed by McAb/C6-H8 was used to screen the allantoic fluid of NDV, and the results showed that McAb/C6/H8 react only with NDV Genotype VI strains, while not with the other strains.

用McAb／C6-H8建立的间接ELISA法检测病毒的尿囊液，结果表明：McAb／C6-H8仅与基因Ⅵ型的毒株反应，而不与其它毒株进行反应。

Firstly , the V4 stock was purified three times by plaque formation. It's difficult to see clear plaques in CEF cells due to the avirulent characteristic , so we treated the V4 stock with trypsin and then allantoic Fluid and Mg2+ were added in the layer gel. In this case, we could see small plaques occasionally, but not stably.

本实验首先对实验室保存的V4株进行了三轮的蚀斑纯化，由于V4株属无毒株，其在CEF细胞中很难形成可见的蚀斑，因此我们采用了用胰酶处理V4病毒，并在上层胶中添加尿囊液及Mg2+，这样可以偶尔产生可见的蚀斑，但实验结果重复性很差。

The amniotic fluid and allantoic fluid of chicken embryo have been analyzed and the resonances of most substances in them were assigned by 1-dimention and 2-dimention Nuclear Magnetic Resonance methods. This work could be the basis of quantitative analysis of metabolites and studying the metabolites changing between the amniotic and allantoic fluid during the hatching process. Also, it provided a new way to study the process of embryo developing.

用一维及二维液体高分辨核磁共振(Nuclear Magnetic Resonance，NMR)方法综合分析了鸡胚羊水和尿囊液的成分，对其中的大多数谱峰进行了归属，发现了一些未经报道的小分子代谢物，为研究胚胎发育过程中各个胚囊中体液的交换和小分子的代谢以及进一步对发育过程中代谢物含量变化的定量研究打下了基础，并为研究胚胎发育的过程提供了新的思路。

The avian influenza virus RNA was directly extracted and purified from chicken em-bryo allantoic fluid. According to published gene sequence,a pair of specific primers to the nucleoprotein gene of AIV was designed and synthesized. After that,the NP cDNA was amplified by RT-PCR .Then the complete NP gene was sequenced.

直接从鸡胚尿囊液中提取H9N2亚型禽流感病毒的RNA，并根据已发表的A型流感病毒株的核苷酸序列，设计了1对特异引物，采用RT-PCR技术成功地扩增了AIV的NP基因；将NPcDNA克隆后进行了序列测定。

The PCR products appeared to be approximate 600 bp, the expected size. The allantoic fluid of infected embryonating eggs of 6 IBV reference strains and 6 field strains were amplified by RT-PCR using three pairs of primers of 1.7, 0.2, 0.6 kb on same and different condition, 3, 5, 9 strains of IBV were amplified respectively. While 12 different sentypes of the 12 IBV strains can be divided in 6 genotypes.

用1.7、0.2、0.6 kb 3对引物对6个IBV毒株和6个分离株的含毒尿囊液在相同和不同条件下进行RT-PCR，结果3对引物分别扩增出3、5、9株IBV，同时可将不同血清型的12个IBV株分成6种基因型。

Virus was also cultured in allantoic cavity of chicken embryo with statistically significant HA titer at 1:6.8 as compared with virus control group (P.01) incubated 24 hours after injected with MEK first then injected with IBV.

在鸡胚尿囊腔中，先注入MEK孵育24 h后，再注入B型流感病毒的鸡胚也培养出病毒，HA滴度为1：6.8，与病毒对照组比较P.01，有统计学意义。

Methods The chicken chorioallantoic membrane assary was used to detect the effect of NDGA on angiogenesis.

采用鸡胚绒毛尿囊膜试验，检测NDGA对血管生成的影响。

In lungs, AQP1 was abundant in peribronchiolar capillaries, postcapillary venules, bronchiolar basal membrane and alveolar epithelial cells.

结果表明，托吡酯在给药剂量范围内（0.068μg-6.8μg／鸡胚）可抑制鸡胚尿囊膜的血管生成，剂量为0.68μg／鸡胚和6.8μg／鸡胚时抑制效果明显，最高抑制率达33.5％。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。