尿囊

The strain of this virus was a strain which could not grow in chicken embryo and could not agglutinate chicken RBC.

此病毒为一种在鸡胚尿囊腔不生长，不凝集鸡红细胞的"O"相甲型流感病毒。

The life-cycle of C.baileyi in Beijing duck and in the CAM of chicken embryowas studied with both the light-microscope and the Nomarski interference-contrastmicroscopy.The results indicated that the life-cycle within duck was simillar tothat of CAM.It included excystation, merogony,gamogonyand sporogony.The thin-walled oocyst was first observed and described in China.

借助普通光学显微镜和微分干涉显微镜对C.baileyi在北京鸭体内的生活史和在鸡胚绒毛尿囊膜上的生活史进行了较详细的研究，结果表明：C.baileyi在北京鸭体内和在CAM上的生活史相同，主要包括"脱囊—裂殖生殖—配子生殖—孢子生殖"四个阶段。

It shows that the allantoin, which only takes little part in calves, was the main part (about73%~88%) in the cattle's urine, and the xanthine and hypoxanthine which are also take only much little part, can be neglected.

结果发现，试验牛尿中嘌呤衍生物中尿囊素大约为73%~88%，黄嘌呤和次黄嘌呤的含量很小，可以忽略不计，与以往对成年反刍动物的研究结果相似，与同个体犊牛期的研究结果相反。

The distribution, properties,structure and regulation of plant allantoinase were reviewed in detail.

文摘：全面综述植物酰脲降解代谢酶的研究现况，概述植物尿囊素酶的分布、性质、结构，以及调节控制，并简介植物尿囊酸酰胺水解酶和脲基乙醇酸酰胺水解酶的研究近况，同时指出待研究的方向。

METHODS: The different combinations of the biofilm were put on the surface of chick embryo chorioallantoic membrane.

将制备的生物膜置于正对观察窗的鸡胚绒毛尿囊膜表面的血管最少处，然后贴在鸡胚绒毛尿囊膜表面上，封口膜封闭假气室，继续孵育。

In order to study the effect of vascular damage in vivo during PDT, the chicken chorioallantoic membrane tumor model by using stable high GFP-expressing human adenoid cystic carcinoma cell strain is built for the experiment.

为了在活体肿瘤模型上实时监测PDT导致的血管损伤效应，使用稳定高表达绿色荧光蛋白的人涎腺腺样囊性癌细胞株，建立了基于鸡胚尿囊膜的肿瘤模型。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示：未妊娠时，泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达，波形蛋白在子宫内膜基质细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，牦牛子宫任何部位均不表达角蛋白7；妊娠30天左右时，泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达，波形蛋白在子宫内膜基质细胞和内胚层细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，角蛋白7在尿囊细胞内表达，偶尔在腔上皮细胞的细胞核边缘表达；消化法进行原代培养时，组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞；分离得到的子宫内膜基质细胞活率达90%以上，并可在体外传代7次以上；分离得到的子宫内膜腺上皮细胞活率可达85%以上，并可在体外传代5次以上；RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长，维持子宫内膜基质细胞正常生长的FBS添加量为20%，维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

The high sensitivity and specificity of real time RT-PCR were shown by The test result, 10~7 of the diluted allantoic fluid infected by AIV can also be detected, and has no cross-reaction with common Avian virus and Avian vaccines, such as low virulent NDV and IB V vaccine.5 days after the broiler has been injected with AIV vaccine, no matter hypodermal or muscular injection, the real time RT-PCR has not found any AIV nucleotide, indicating the use of AIV vaccine has no effect on the test results.

在检测禽流感感染的尿囊液时，尿囊液稀释至10~(-7)仍可检出病毒核酸，荧光RT-PCR方法的特异性也很强，检测时与禽群常用的疫苗如新城疫弱毒疫苗、传染性支气管炎弱毒疫苗等均不出现交叉反应。

METHODS: Constructing the CAM model by exposing CAM on the shell subpoint between 2 vitelline veins and generating an air chamber after 5 days of incubated with aseptic incubator at 37℃.

将鸡胚消毒后，置于37℃无菌恒温箱中孵育5 d，在距胚头1 cm两条卵黄静脉之间的卵壳投影部位磨切暴露尿囊膜，制成假气室，用无菌滤纸封闭窗口，制成鸡胚尿囊膜模型，备用。

Tri- sodium citrate with aqueous gold chloride were warmed up and mixed to make gold sol; The chicken IgG(anti-JG04 strain GPMV) was immobilized to a defined detection zone on a porous nitrocellulose membrane, while 3A_5 was conjugated to colloidal gold which served as a detection reagent.

在加样处沾取含有GPMV的尿囊液样品，GPMV与金标3A_5结合形成混合液，由于层析原理，混合液向上移动，在检测线上混和液中与金标颗粒结合的GPMV与鸡IgG结合，由于大量GPMV沉积在检测线上，出现红线；而如果尿囊液中不含有GPMV，检测线上不出现红线。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。