尿嘧啶

By the polycondensation of amino acid 5-fluorouracil -1, 3-dipropyl esters with bis (2-chloroethyl) phos- phoramidic dichloride in the presence of 〓 or in vacuo, ten new polymers containing 5- fluorouracil, nitrogen mustard and amino acid were synthesized.

将1，3-双-5-氟尿嘧啶与二氯膦甲酸乙酯或二氯膦乙酸乙酯在DMF中反应制备得到20个三元共聚物，体外抗肿瘤试验表明：含有甘氨酸、缬氨酸、苯丙氨酸和酪氨酸的聚合加显示了一定的选择性，并且分析讨论了生物活性与结构之间的关系。

Piperate and methyl piperate exhibited a good protecting effect on cardiac myocytes while uridine had not activity.

对H202诱导的心肌细胞损伤，胡椒酸甲酯和胡椒碱具有明显的保护作用，咖啡酸甲酯具有部分保护作用，尿嘧啶苷无保护作用。

Seven polyamides containing 5-fluorouracil in the side chain were synthesized by the condensation of di-p-nitrophonyl N-(5-fluorouracil-1-acetyl)- glutamate with a series of polymethylene diamines and lysine ethyl ester.

用1，3-双-5-氟尿嘧啶和双（2-氯乙基）氨基磷酰二氯，在DMF中反应合成了10个含三个组份的聚合物。

The recombinant Hansenula polymorpha strains for secretory expression of HBsAg were screened and cultured in YPD, BMMY and MM media respectively. The expression levels of HBsAg were determined by ELISA, and the virus-like particles were observed by transmission electron microscopy.

将其电转化多型汉逊酵母尿嘧啶缺陷型宿主菌ATCC34438(Ura3-)，筛选分泌表达HBsAg的汉逊酵母工程菌株，并比较在YPD、BMMY、MM3种培养基中分泌表达HBsAg的水平，ELISA检测培养上清液中HBsAg的表达量，透射电镜观察类病毒颗粒。

Pombe which is not responsible for pseudouridylation of rRNA or snRNA. At last, genetic analyses of the functions of one pseudouridylation guide and two orphan guide snoRNAs were carried out by gene deletions.

最后，本文还利用同源重组方法对1个指导假尿嘧啶化修饰的功能snoRNA分子SP20和两个orphan-guide snoRNA分子SP12和SP16进行了基因缺失的遗传学分析。

Methods:Cardiac muscle of twoday neonate rattus was digested repeatedly by 0.0625% trypsin.The cardiocytes were collected in DMEM medium,which contains 10% fetal bovine serum.Myocardial cells were isolated with the technique of differential anchoring velocity with 90 min and were pured by Brdu.The cardiocytes were placed into CO2 incubator to incubate seven days after their purification.

应用0.0625%的胰蛋白酶重复消化出生第2 d乳鼠的心肌组织多次，收集的细胞用含10%胎牛血清的DMEM培养基中和，用差速贴壁分离法分离，在以溴脱氧尿嘧啶纯化心肌细胞后置CO2培养箱孵育7 d。

Using the MTT assay, agarose gel electrophoresis, transmission electron microscope, TUNEL method and immunohistochemistry (PCNA, bcl-2),it was studied in this experiment that the inducing APO, anti-proliferation and affection of expression of bcl-2 gene before and afterAPO of the Rubia Cordifolia L with the 5-fluorouracil(5-fu) for MGC-803 cells of gastric carcinoma.

本实验通过MTT法、琼脂糖凝胶电泳法、透射电镜观察法、TUNEL法、免疫细胞化学法（PCNA、bcl-2），研究了茜草提取物联用5-氟尿嘧啶（5-fluorouracil，5-Fu）对胃癌MGC-803细胞株的诱导凋亡、抗增殖作用及凋亡前后bcl-2基因表达的影响。

Methods 5-FU, MTX, CDDP in different concentrations were given to every group after transfected with Cyclin D1 SOND or ASODN for 24 h ,the do~-effects response was observed and 1C50 was calculated in every group.

在给予Cyclin D1 ASODN和Cyclin D1反义寡核苷酸转染细胞24h后，分别给予梯度浓度的5-氟尿嘧啶（5-FU）、氨甲喋呤、顺铂，观察各组的量效反应，计算IC50。

After the type 1 diabetic encephalopathy rat models were set by streptozotocin for 5 days, insulin and saline were used respectively to treat the related model rats for 55 consecutive days. Morris water maze and bromodeoxyuridine immunohistochemistry were used to detect the changes in spatial memory and learning, and the neural stem cell proliferation in the subgranular zone of hippocampal dentate gyrus of each rat group.

在用链脲佐菌素建立1型糖尿病脑病模型后的第5天，分别用胰岛素和生理盐水替代治疗55天，再通过Morris水迷宫测试及5-溴脱氧尿嘧啶（BrdU，神经干细胞DNA合成的标记物）免疫组织化学方法，分别观察和比较各组大鼠SGZ神经干细胞增殖和空间学习记忆的动态变化。

Objective To investigate the effect of Matrine infections on LoVo cell and its synergetic effect with 5-Fu.

目的探讨复方苦参注射液与5氟尿嘧啶对大肠癌LoVo细胞生长抑制及诱导凋亡的影响。

Shop assistant: Please check at the door.

店员：请您到门口柜台交费。

In the end, transportation of PAHs in the environmental matrix was preliminarily investigated and discussed according to PAHs data of soils and sediments from a certain region in Shanghai. The result indicated that there are two or more pathways on PAHs transportation in the environmental matrix.

最后，对上海特定地区内PAHs在环境介质中的迁移径途进行了分析讨论，结果表明上海市土壤和沉积物等环境介质中PAHs的迁移存在多种途径。