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In the third chapter, the ultrastructure of cercaria is introduced, the feasibility of using high-voltage pulsed electric field to eliminate schistosome is discussed, elimination mechanism of high-voltage pulsed electric field is researched and the factors which affect the elimination effect are analyzed.

第三章主要介绍尾蚴的超微结构,探讨采用血吸虫尾蚴高压脉冲电场杀灭技术的可行性,研究血吸虫尾蚴高压脉冲电场杀灭技术的机理,分析影响杀灭效果的电场参数、处理环境和生物特性因素。

It is the most powerful means of eradicating schistosomiasis. Current method of elimination cercaria is mainly the chemical method which has many disadvantages such as drug resistance, drug dilutedness, environmental pollution and poisoning the other biology, etc. For these reasons, this paper poposed a new method of elimination through high-voltage pulsed electric field, discussed the feasibility of the new method, studied the elimination mechanisms of using different forms of electrodes, analyzed various factors which influences the effect of elimination and introduced the structure of high-voltage pulsed electric field system at the stage of laboratory. Moreover, this paper showed the security problems that should be considered during the research and experiment.

针对常用的化学药物杀灭法存在的易使尾蚴产生抗药性、水域宽广致使药效降低、污染环境、毒杀其它生物等弊端,本文提出了一种利用高压脉冲电场杀灭血吸虫尾蚴的新方法,探讨了这种电子杀灭方法的可行性,分别研究了采用锐形电极和平板电极的血吸虫尾蚴杀灭机理,分析了影响尾蚴杀灭效果的各种因素,介绍了实验阶段血吸虫尾蚴高压脉冲电场杀灭系统的构成,指出了系统研制和试验过程中需要充分考虑的安全性问题。

The author studied firstly, systemtically and roundly on the membrane molecular biology of Cysticercus cellulosae during development, and put forward the biomembrane system, chemical compose, the process of phosphorus metabolism and biomembrane characteristic, and their changes during development.

首次对猪囊尾蚴膜分子生物学进行了较系统全面的研究,提出了猪囊尾蚴的生物膜系统、生物膜化学组成、膜磷脂代谢的途径及其生物膜特性以及发育过程中的变化。

ResultsNone was infected in the Ginger oil group, the infection percentage of the muddy water group was 10%, and the rates of worm reduction were 100% and 88.5%, respectively.

结果A组小鼠血吸虫尾蚴的感染率为0,减虫率达100%; B组小鼠感染率为10%,减虫率达88.5%;两组结果与对照组比较血吸虫尾蚴感染率差异均有显著统计学意义(P<0.001)。

Methods Cryostat sections for adult worms and paraffin sections for eggs in the liver of infected mouse, sporocysts and cercariae in snails were prepared, immunofluorescent test was performed to detect the presence of iNOS in adult worms, sporocysts, cercariae and miracidium, the distribution of this enzyme was observed in different stages of Schistosoma japonicum. Western blotting was used to further demonstrate the immunoreactivity to iNOS in adult worms.

收集日本血吸虫成虫制成冰冻切片,将含胞蚴、尾蚴的感染性钉螺和含成熟虫卵的小鼠肝脏制成石蜡切片,用免疫荧光法检测成虫、胞蚴、尾蚴以及毛蚴中的iNOS,并观察该酶在各期虫体内的分布;用蛋白质印迹法检测成虫匀浆中的可溶性蛋白是否具抗iNOS多抗的可溶性蛋白。

It consist the three developments of Teania solium, Oncosphere and Cysticercus cellulosae, that the Oncosphere takes an important role in inbreaking body and causing response of immunology.

猪带绦虫的生活史由成虫、六钩蚴和囊尾蚴三个发育阶段组成,六钩蚴是囊尾蚴的早期发育阶段,在入侵宿主及引起机体免疫学反应等方面扮演重要角色。

Albendazole and oxfendazole both inhibited the pathways of anaerobic glycolysis, partial inversed tricarboxylic acid cycle of Taenia Solium oncosphere, immature and mature cysticerci in vitro and immature and mature cysticerci in vivo in certain degree. They enhanced decomposing in fat, amino acid, and xanthine metabolism. On the other hand, absorption of glucose was hindered.

阿苯哒唑和奥芬哒唑对体外培养猪六钩蚴,未成熟期和成熟期猪囊尾蚴,以及体内发育未成熟期和成熟期猪囊尾蚴的糖无氧分解途径,丙酮酸羧化支路与逆向三羧酸循环途径均有一定程度抑制作用,代偿性促进糖异生及蛋白质,脂类和核酸分解途径,葡萄糖吸收受阻。

The cercariae′s tegumental has secreted and excreted function. The tail bin of it was described,which can help it working in the water.

尾蚴的体被具有分泌、排泄功能;尾蚴尾部有鳍,有助于尾蚴在水中活动。

Methodsthe Ginger oil were loaded on the slides, and the time of killing the Schistosoma Cercariae was recorded.

取生姜油100μl于载玻片上,观察生姜油杀灭血吸虫尾蚴的时间;并用已杀灭的血吸虫尾蚴感染小鼠,经灌注法加撕碎法收集虫体,计算感染率。

In this test, metabolites and enzyme activities involved in energy metabolism in Cysticercus cellulosae were determined systematicly in vivo and in vitro and under action of drugs.The principles of variation of energy metabolism in Cysticercus cellulosae and mechanism of action of the benzimidazol carbamate drugs were illustrated so as to select an effective drug against immature and mature cysticerci and to provide a theoretical and experimental chemotherapy.

本研究首次系统地测定了体内发育过程中、体外培养条件下及抗囊药物作用下猪囊尾蚴能量代谢的有关物质含量和酶活性的变化,阐明了猪囊尾蚴的能量代谢变化规律和苯并咪唑氨基甲酸酯类药物作用机理,为筛选有效的抗囊药物以及为药物治疗提供了重要的实验和理论依据。

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