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A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物,经过扩增前导序列10~34aa基因序列,并重新克隆入质粒pRSET-A构建串联二聚体后,再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点,经一系列简单的酶切和连接,快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因,并成功表达其六聚体重组蛋白。

Objective To explore the feasibility of retrograde distal pancreatectomy starting from the neck of the pancreas in the treatment of pancreas tumor.

目的 探讨胰体肿瘤行先切断胰颈的逆行性胰体尾切除方法的可行性。

A new method for measuring surface area of flatfishes was presented in this paper. Digital camera is used to capture digital images of flatfishes with a background of standard plotting paper, image processing softwares (Photoshop CS2 or Adobe Acrobat Professional) are used to get pixels per square centimeter and the pixels of flatfishes, then surface area of flatfishes can be calculated by these data. Compared with traditional method, results of surface area measuring of 30 turbot revealed that relative errors of Photoshop Method and Acrobat Method were -4.40% to 3.08% and -1.83% to 1.30%, respectively.

提出一种快速、准确测定鲆鲽类体表面积的新方法,利用数码相机拍照获取带有标准坐标纸背景的鲆鲽类鱼体数码图像,采用图像处理软件Photoshop CS2和Adobe Acrobat Professional分别获得单位背景面积和鱼体图像的像素数,通过两者的比例计算出体表面积。30尾大菱鲆体表面积的实测表明,与传统计数方格面积法的测量结果相比,Photoshop法相对误差为-4.40%~3.08%,Acrobat法相对误差为-

This "pair" is easy to tell from other warblers by combination of greyish crown, green upperparts, narrow and very white wing bars, rather white supercilium and rather clean white underparts, pink legs, more skulking habits and frequently-given whistled "tsUUI" notes which to my ear sound very like the call of Daurian Redstart.

这两种柳莺可以和其他柳莺很容易区别,透过它们灰色的冠,绿色的体上,很细又很白的翼带,还算白的眉线,还算乾净、白色的体下,粉红色的脚,喜爱躲藏的习性,以及经常听到,且对我来说很像黄尾鸲叫声的「tsUUI」声音等特徵。

A small sturdy dog of an ancient breed originating in China,having a snub nose,a wrinkled face,a squarish body,short smooth hair,and a curled tail.

哈巴狗源于中国的一种鼻部短而扁平、面部多皱纹、体方、毛短而光滑且尾卷的品种古老的小体健壮的狗。

Liaoning Medical University, Jinzhou 121001 China)Abstract:Objective To study the distribution and liver targeting of glycyrrhetinic acid derivatives (stearin glycyrrhetinic acid ester-3-O-galactosidase, Gal-GAOSt) modifying norcantharidin liposomes (Gal-GAOStNC-LP) in rats.

目的 研究甘草次酸衍生物(甘草次酸硬脂醇酯-3-O-半乳糖苷,Gal-GAOSt)修饰去甲斑蝥素脂质体(Gal-GAOStNC-LP)在小鼠体内的分布及肝靶向性。方法利用薄膜分散法制备甘草次酸衍生物修饰去甲斑蝥素脂质体后,经小鼠尾静脉给药,采用HPLC法检测去甲斑

These results have indicated that the telencephalon of the subadults had the roughly similar structure with the adults, and that Onychodctylus fischeri is a relatively primitive type in amphibian. This research, at some extent, has filled in the blank space data in nerve system and nerve development of tailed amphibian type.

这些都表明亚成体爪鲵的端脑已大致具备了成体所具有的结构,并且也说明了爪鲵在两栖纲动物中属于较原始的种类,这在一定程度上填补了有关有尾两栖类神经系统资料以及神经发育资料的空白。

The DNA contents of the majority of multiple pengze crucian carp studied were close to that of tetraploidy, and the DNA contents of the minority of multiple pengze crucian carp were between that of triploid and tetraploidy.

结果显示,复合鲫的DNA含量明显多于母本种彭泽鲫的DNA含量,且所测14尾中多数个体的DNA含量超过或接近四倍体的DNA含量,少数个体的DNA含量介于三倍体和四倍体之间。

First, each morphometric character is related to fork length by linear regression analysis and each relationship is considered as a unit character.

首先,以每一非体节形质对尾叉长做廻归判定,其次,以变积分析法比较各个非体节形质,在雄雌间及各海域标本间之异同。

A new subdivision "marginal division",was found located at the caudal most edge of the neostriatum, near the rostral border of the globus pallidus in the brains of rat.

纹状体边缘区是舒斯云自1987年来先后在大鼠、猫、猴及人的新纹状体尾内侧、靠近苍白球边缘处发现的一个由梭形细胞构成的新亚区。

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Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势,并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。

This dramatic range, steeper than the Himalayas, is the upturned rim of the eastern edge of Tibet, a plateau that has risen to 5 km in response to the slow but un stoppable collision of India with Asia that began about 55 million years ago and which continues unabated today.

这一引人注目的地域范围,比喜马拉雅山更加陡峭,是处于西藏东部边缘的朝上翻的边框地带。响应启始于约5500万年前的、缓慢的但却不可阻挡的印度与亚洲地壳板块碰撞,高原已上升至五千米,这种碰撞持续至今,毫无衰退。