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I think there are too many shallow rich bratty people there, but everybody eventually finds people they get along with.

学校有一些肤浅令人讨厌的有钱人。共有 1条回复信息,1 页,每页 10 条,当前为第 1页首页上一页下一页尾页

In this paper, the analytical expressions of seismic input for homogeneous elastic half-space are first derived in oblique incidence of plane SV-wave, and then a finite element model for a real hydropower cavern group composed of main powerhouse- transformer chamber- tailrace surge tank cavern is created. Characteristics of seismic responses such as displacement, acceleration and stress are studied in detail at different incidence angles and some results are compared with those given by the literature.

本文首先推导了平面SV波斜入射下均质弹性半无限空间的地震动输入表达式,然后建立了某水电站主厂房室-主变室-尾水调压室等构成的地下洞室群有限元模型,详细研究了不同入射角度下各洞室的位移、加速度、动应力等地震反应特点,并与文献进行了对比。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank,选择包含人主要T细胞抗原表位序列的人PSCA基因片段,应用异种化抗原设计技术,保留人T细胞抗原表位,设计异种化PSCA融合抗原片段;(2)根据核酸序列按中心模板法设计引物,应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因,以PCR、限制性酶切和DNA序列测定法进行鉴定:(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点,采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4),并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中,转染293T细胞,借助免疫荧光+流式细胞术考察插入片段表达效率,最终选定PSCA_3片段进行下一步研究;(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下,插入pVAX1-neo-IRES—GM/B7载体中,构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB,并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况;(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞,制备小鼠前列腺癌模型,并采用股四头肌肌肉注射+电脉冲法(Electroporation,EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB,同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照,通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率,来评价该DNA疫苗在小鼠体内的抑瘤效果。

The results show that Cd2+ for Pistia stratiotes and Myriophyllum spicatum L root-like effects of microbial flora with the microorganism or physiological groups, training time and Cd2+ concentration varies; Micro-organisms under Cd2+ stress would have two distinct effects: First, the growth of micro-organisms are not suited to reduce the number or extinction; Second, the growth of micro-organisms adapt to an increase in the number or the accumulation.

结果表明,Cd2+对大薸和穗状狐尾藻根系微生物的影响随微生物区系或生理类群、培养时间和 Cd2+浓度的不同而不同;受重金属镉胁迫后微生物会产生两个明显的效应:一是不适应生长的微生物数量会减少或灭绝;二是适应生长的微生物数量会增加或积累,在低浓度下,重金属对微生物数量一般有刺激作用,而在高浓度下一般为抑制作用,也有表现出刺激作用。

Calyciflorus clones were cultured at 15℃, 20℃, 25℃and 30℃with 3.0×106 cells/mL of Scenedesmus obliquus to investigate their differences in life history characteristics and their responses to increasing temperature.

结果表明,温度对萼花臂尾轮虫存活率和繁殖率的影响在不同克隆间存在着差异。20℃和25℃下,四个克隆轮虫的世代时间和净生殖率均分别无显著的差异;20℃、25℃和30℃下,四个克隆轮虫的平均寿命和出生时的生命期望亦然。

The invention discloses a treating method of tail water membrane filtering condensation fluid in chemical industry park, the steps of which are that the pre-treated membrane filtering condensation fluid goes through a device filled with resinous adsorbent under the common temperature and a flow quantity of 0.5-8BV / h, the absorbed water can be discharged after neutralizing with small capacity of alkali, desorbing and regenerating the resin by natrium hydroxydatum solution with the flow capacity of the desorbing agent is 0.5-3.0BV / h, the high concentration desorbing fluid disposes safely and the low concentration desorbing fluid can be periodically used to preparing next pluralities of desorbing agent.

本发明公开了一种化工园区尾水膜滤浓缩液的治理方法。其步骤为:将预处理后的膜滤浓缩液在常温和0.5~8BV/h的流量条件下,通过装填有树脂吸附剂的装置,吸附出水加少量碱中和后即可排放;将上述树脂用氢氧化钠溶液脱附再生,脱附剂流量为0.5~3.0BV/h,高浓度脱附液安全处置,低浓度脱附液可用于配制下一批脱附剂循环套用。

The dog clutch, of course, provided an instant release when machining under power (and many owners made up simple automatic knock-off devices to allow exact turning up to a shoulder) but a second reason behind its fitting was a practical difficulty unmentioned by the makers: as owners quickly discovered, when screwcutting with the help of the headstock clutch it was necessary for the carriage to be wound back manually using the wheel at the tailstock-end of the leadscrew whilst the changewheels remained in mesh.

狗离合器,当然,提供了一个即时释放下加工时功率(和很多业主提出了简单的自动敲小康装置,使转向精确到肩膀),但第二个原因是其拟合实际困难未提及的制造商:作为业主很快发现,当screwcutting的帮助下启闭离合器有必要的运输必须使用手动伤口回到车轮在尾座年底的丝杠,而留在changewheels网格。

This thesis investigated the VOC emission from drying of Chinese fir and Eucalyptus plantation lumber in a pilot-scale kiln.

本论文以杉木、尾叶桉为研究对象,对木材精油和木材生材在常温状态下释放的有机挥发物成分进行了分析研究,重点研究了木材在高温和常规干燥条件下VOC释放成分,释放规律及影响因素,评价了木材干燥过程中VOC释放对环境的影响,为有效合理控制VOC排放和制定其排放标准提供了基础数据。

The hull (4) has an abruptly down-swept trailing edge portion (18) which, in the preferred embodiment, is in the form of a flap extending across the full width of the transom of the hull and which projects beyond the level of an upswept portion of the underside (14) of the hull by only a small fraction of the length of the hull.

该船体(4)具有一个突然下掠的后缘部分(18),在优选实施例中,该后缘部分呈横过船体尾板全宽延伸的折翼的形式,并且该后缘部分超过船体下侧面(14)的上掠部分的水平只伸出一个船体长度的一个小部分。

Sea perch embryos were cryopreservated in liquid nitrogen for 0.5~24h, 4 survival embryos were also obtained and one of them hatched, furthermore the fry without feeding survived for 3 days.

其中妒鱼胚胎成活4枚,孵化1枚,孵化后的妒鱼苗在没有投喂饵料的情况下存活了3天(在液氮中保存了28h);牙醉胚胎成活5枚,孵化4枚,孵化后其中l尾牙虾苗在没有投喂饵料的情况下存活了6天(在液氮中保存了24h)。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?