少突神经胶质

Nodes of Ranvier; saltatory conduction; Schwann cells; oligodendrocytes; myelin basic protein; demyelination; remyelination; myelinopathy; myelinogenesis; myelin-associated protein; myelin sheaths; demyelinating neuropathy; myelin proteolytic protein; chronic inflammatory demyelinating polyradiculoneuropathy; multiple sclerosis; MS

郎飞氏结；跳跃传导；施旺细胞；少突神经胶质细胞；髓鞘碱性蛋白脱髓鞘；髓鞘再生；髓鞘质病；髓鞘形成；髓磷脂-关联的蛋白质；髓鞘；脱髓鞘神经病；髓磷脂溶解蛋白的蛋白质；慢性炎症性脱髓鞘多神经根神经病；多发性脑硬化；MS

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

The brain tissue of the different fetal age rat could be cultured into neural stem cells with the most number of cells for 12.5 dags embryo, which furthermore differentiated into neurons and glial cells.

胎龄为12.5天胎鼠大脑皮质培养出的神经干细胞数量最多，可分化为神经元、神经胶质细胞及少突胶质细胞。

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此，本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs，使用无血清培养基进行扩增、培养，用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定，对少突胶质系细胞表达部分营养因子的情况进行检测；采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测；将OPCs移植入成年SD大鼠玻璃体内，利用上丘逆行荧光标记技术，观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间；将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内，观察不同时期视网膜内髓鞘形成与分布特点，分析髓鞘的超微结构，并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

Results: ApoD was observed positively expressed in type2 astrocytes and oligodendrocytes from O2A lineage, while it was not expressed in type1 astrocytes from T1A lineage. Accordingly, we can verify the phenomenon found in the study of T1A and T2A gene different expression profile that ApoD mRNA was highly expressed in T2A and lowly expressed in T1A. Conclusion: It can be concluded that lipid metabolism related genes such as ApoD play important roles in the generation and differentiation of O2A lineage, which closely correlate with internal mechanism of lipid metabolism in the brain and myelogenesis.［Key words］astrocyte(T1A, T2A); oligodendrocyte; O2A lineage; apolipoproteinApoD

我们已采用基因芯片技术在mRNA水平研究两型星形胶质细胞的基因表达谱差异，发现ApoD在2型星形胶质细胞中差异高表达，故本文拟用激光共聚焦双重免疫荧光标记技术进一步在蛋白质水平观察ApoD在体外分化成熟的两型星形胶质细胞以及少突胶质细胞中的表达情况，以期为我们深入研究ApoD等脂代谢相关基因在脑内脂质代谢和神经髓鞘发育机制中所起的作用提供有用的实验依据，依此探讨胶质细胞发生和分化的信号机制。

Neuroglial cells (oligodendrocytes and astrocytes) play a very important role in establishment and maintenance of normal neuronal functions and formation of neurological diseases.

神经胶质细胞（主要是少突胶质细胞和星形胶质细胞）对于正常神经系统功能的建立和维持及神经系统疾病的产生都起着至关重要的作用。传统观念认为大脑功能的唯一发挥者是神经元。

Rasagiline as an adjunct to levodopa in patients with Parkinson＇s disease and motor fluctuations （LARGO, Lasting effect in Adjunct therapy with Rasagiline Given Once daily, study）: A randomised, double-blind, parallel-group trial, Incidence of neural tube defects in the leastdeveloped area of India: A population-based study,Long-term efficacy of early versus delayed radiotherapy for low-grade astrocytoma and oligodendroglioma in adults: The EORTC 22845 randomised trial

文章摘要：雷沙吉兰作为左旋多巴的辅助用药治疗帕金森病和运动波动患者（LARGO研究。雷沙吉兰每日1次辅助治疗的持久影响）：一项随机、双盲、平行分组试验，神经管缺陷在印度最不发达地区的发病率：一项基于人群的研究，早期和晚期放疗对成人低分级星形细胞瘤和少突神经胶质细胞瘤的长期疗效：EORTC22845随机试验

The St. Jude team first determined that CSCs are located in ascular niches by identifying cells carrying a protein called Nestin that marks stem cells (Nestin+ cells) in four types of brain cancer remoed from patients: medulloblastoma, ependymoma,oligodendroglioma and glioblastoma.

圣犹达研究组研究了病人的四种脑肿瘤：成神经管细胞瘤、室管膜细胞瘤、少突神经胶质瘤和成胶质细胞瘤，通过鉴别名为Nestin的蛋白来识别肿瘤干细胞，第一个验证了肿瘤干细胞存在于血管性的微环境中。

Curran and a team of American and Canadian researchers, in conjunction with the Philadelphia-based Radiation Therapy Oncology Group, a federally-supported clinical trials organization, found that among 289 patients with either anaplastic oligodendroglioma or anaplastic oligoastrocytoma receiving either a combination of three chemotherapy drugs with radiation or radiation alone, those whose tumors had deletions in chromosome locations 1p and 19q tended to live nearly two and a half times longer than those patients without the missing chromosome portions.

临床癌症杂志》上周报道，卡仑博士和由美国、加拿大研究人员组成的科研小组，联合了总部设在费城的由联邦政府支持的临床实验组织－美国放射肿瘤学协作组，进行了研究。289名病人患有退行性少树突神经胶质瘤或退行性少星细胞瘤，研究发现，这些病人在接受三种化疗药物结合放疗或者只接受放疗后，其中染色体缺乏1p 和19q 的病人比那些不缺乏的病人多存活了2倍半的时间。

Human hair keratin ; spinal cord injury ; rat ; tissue engineering ; tissue-engineered spinal cord ; connector ; morphology ; praxiology ; neuron ; axis-cylinder ; nerve fiber ; myelin sheath ; astrocyte ; oligodendrocyte ; microglia ; ependymal cell ; neural stem cells ; macrophage ; multinuclear giant cell ; lysosomal enzyme ; ubiquitin system ; suppression subtractive hybridization ; gene express ; CytohromeoxidaseⅢ; amino acid transporter system A2（Ata2）; Peroxiredoxins 5（prd 5）; cysteine proteinase inhibitor cystatin c

国家自然科学基金；广东省自然科学基金人发角蛋白；脊髓损伤；大鼠；组织工程学；组织工程化脊髓；桥接物；形态学；行为学；神经元；轴突；神经纤维；髓鞘；星形胶质细胞；少突胶质细胞；小胶质细胞；室管膜细胞；神经干细胞；巨噬细胞；多核巨细胞；溶酶体酶；泛素肽酶系；抑制消减杂交；基因表达；细胞色素氧化酶Ⅲ；氨基酸转运蛋白系统A2；抗过氧化物蛋白5；半胱氨酸蛋白酶抑制剂

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。