少数

B Of the six basic media MS, MS1/2 (half-strength of MS salts and vitamins), WPM, DKW, B5 and SH, MS1/2 was the most proper one to induce somatic embryos. Somatic embryos generally regenerated directly from excised zygotic cotyledons. PGRs combination affected somatic embryogenesis significantly. Medium with NAA 1mg/L, TDZ 0.05mg/L, IBA 2—10mg/L combined with BA 10mg/L, or IBA 10mg/L integrated with BA 0-2mg/L gave the highest induction rate. Excised zygotic hypocotyls had the strongest potential to produce callus. Callus induction was also affected significantly by media and PGRs. The proper callus induction condition was MS1/2 medium containing NAA 1mg/L, IBA 10mg/L, BA 2-5mg/L and TDZ 0.05mg/L. Harvest period affect somatic embryogenesis significantly. Zygotic embryo explants collected from the end of July to the middle of August had strong potential to generate somatic embryos, when endosperm finished solidification, different parts of the embryos were completely formed, the size of embryos occupied about 2/3 of the embryo sac. Provided with optimized conditions, direct somatic embryogenesis rate can attain to 33. 68%, and callus induction rate of hypocotyls was up to 90.7%. Cytological observation on megasporogenesis and zygotic embryogenesis of Manchurian ash showed that the ovary was twicarpellum, twilocular with two ovules each loculus. The ovule was tenuinucellar and anatropous, with one megasporcocyte. The development of embryo sac is of the Polygoum type.

体细胞胚胎发生研究的结果表明：(1)成熟过程中的合子胚是诱导水曲柳体细胞胚胎发生的最佳外植体材料；(2)在所试验到的MS、MS1/2（将MS的所有成分均减半）、WPM、DKW、B〓、SH等六种基本培养基中，MS1/2是最适合诱导水曲柳体细胞胚胎发生的基本培养基；(3)水曲柳的体细胞胚胎发生以直接发生为主，体细胞胚主要来自于从合子胚分离的完整子叶；(4)培养基中的激素组合对水曲柳的体细胞胚胎发生有显著影响，诱导直接体细胞胚发生较好的激素组合有NAA 1mg/L+IBA 2，5，10mg/L+BA 10mg/L+TDZ 0.05mg/L和NAA 1mg/L+IBA 10mg/L+BA 0，2mg/L+TDZ 0.05mg/L；(5)合子胚分离的下胚轴具有最强的愈伤组织诱导潜力，少数愈伤组织可以分化出体细胞胚；(6)愈伤组织的诱导也受培养基和激素配比的显著影响，最适宜诱导的培养条件为MS1/2+NAA 1mg/L+IBA 10mg/L+BA 2，5mg/L+TDZ0.05mg/L；(7)采种时间对体细胞胚胎发生有显著影响。7月末到8月中旬的合子胚具有较强的体细胞胚发生潜力，此时种子尚未成熟，胚乳已呈固态，种胚的各个部分已分化完全，种胚体积占胚腔的大约2/3；(8)在各自综合的最适条件下，完整子叶的体细胞胚诱导率可达33.68%，下胚轴的愈伤组织诱导率可达90.7%。

If you're one of the lucky few to own one look us up for a test drive!

假如你是厄运的少数拥有自己的一，寻找试驾我们！

Lovecraft's readership was limited during his life, but his works have become quite important and influential among writers and fans of horror fiction.

在他生前，其著作仅有少数的读者；但在他离世后，其著作成为在恐怖小说作家及爱好者中相当重要并具影响力的作品。

Most people solve the accommodation needs of low-end market has been very few and can not be regulated.

大多数人解决了住房需求的低端市场一直是极少数，不能调节。

Nuclear DNA content analysis revealed that hybrid plants had different nuclear DNA content:higher,lower than the parental sum or equal to it.Contrast to the lower and equal hybrids,bigger amount of hybrids with higher DNA content had abnormal leaf,bad growth vigour and poor fertility.

结果表明：杂种形态变异广泛，多数呈中间类型，少数呈偏亲性状；杂种细胞DNA含量不一致，呈现大于、等于、小于双亲细胞DNA含量之和的类型；DNA含量大于双亲之和的杂种与DNA含量等于或小于双亲之和的杂种相比，有较高比例的材料叶型出现畸形和植株生长势弱，育性也更差。

The handful of foreign reporters granted visas were carefully herded to points from which only loyalist rallies could be viewed.

获得签证的少数外国记者只能集中到那些精心挑选的只有忠诚分子集会的地点。

Luckily, I am in the minority.

幸运的 ，我在少数。

Along with English botanist Joseph Hooker, Lyell was one of a small handful of people Darwin had shown early drafts of his own work on natural selection.

与英格兰植物学家霍克一道，赖尔是达尔文早先把自己关于自然选择的著作草稿拿给他们看的少数人之一。

Only a few musicians know how to strike a lyre.

只有少数的音乐家会弹七弦琴了。

Qin told me with great enthusiasm that his studio at MacDowell was the first time he was able to work in large scale.

我在麦克道尔基金会任职期间曾认识许多艺术家，但只有极少数艺术家的作品具有超越性的宇宙意义。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。