小鼠

BALB/c mice were immunized with plasmid TR421-hCGβ or mock DNA three times. Two weeks after immunization, spleen cells from the immunized mice were harvested, and then analyze the CTL activity against Sp2/0-ehCGβ cells. The splenocytes derived from the immunized mice were adoptively transferred to the normal mice, which were subsequently given injections i.

以TR421-hCGβ质粒实施基因免疫，免疫后检测小鼠脾细胞，特异性细胞毒活性；同期将脾细胞过继免疫给正常小鼠，以过继TR421-hCGβ质粒免疫小鼠脾细胞为实验组、过继TR421质粒免疫小鼠脾细胞为对照组，检测脾细胞杀伤效应。

ABSTRACT］ Objective To study the pharmacological effects of oral medical ice and to provide for its evidence for clinical application. Methods The anti-inflammatory effect of OMI was determined on three inflammatory models: swelling of mice ear induced by dimethylbenzene, hind paw edema and back granuloma caused by hypodermic injection of agar in rats. The an- algesic action of OMI was studied by observing the mice's reaction to the heat simulation. The effect of OMI on bleeding time was tested by tail-cutting method.

摘要］ 目的探讨口腔医用冰的药理作用，临床用药提供依据方法制备二甲苯致小鼠耳部炎症、琼脂致鼠足肿胀急性炎症模型，大鼠琼脂肉芽肿慢性炎症模型，观察口冰对炎症的作用；采用热板法进行小鼠热刺激性疼痛试验，断尾法测定小鼠出血时间，观察口冰对小鼠疼痛及出血时间的影响。

Methods the anti-inflammatory effect of omi was determined on three inflammatory models: swelling of mice ear induced by dimethylbenzene, hind paw edema and back granuloma caused by hypodermic injection of agar in rats. the an- algesic action of omi was studied by observing the mice's reaction to the heat simulation. the effect of omi on bleeding time was tested by tail-cutting method.

制备二甲苯致小鼠耳部炎症、琼脂致大鼠足肿胀急性炎症模型，大鼠琼脂肉芽肿慢性炎症模型，观察口冰对炎症的作用；采用热板法进行小鼠热刺激性疼痛试验，断尾法测定小鼠出血时间，观察口冰对小鼠疼痛及出血时间的影响。

MethodsMouse twisting numbers induced by acetic acid, mouse pain threshold after heat stimulation on hot-plate and in hot-water tests were observed to investigate the analgesic effects, meanwhile, the contents of prostaglandin E (PGE2) and malondialdehyde and the activity of superoxide dismutase in mice serum and cerebrum were measured to explore primary analgesic mechanisms of calonyction aculeatum beans.

方法采用醋酸致小鼠扭体法，小鼠热板法及小鼠温浴法，观察月光花豆乙醇提取物的镇痛作用，同时检测热板法小鼠的血清和脑组织PGE2、MDA的含量及SOD活性。

ResultsThe ethanol extracts of Calonyction aculeatum Beans could remarkably reduce mouse twisting numbers and prolong pain thresholds, meanwhile, it also reduced the elevated MDA and PGE2 productions and enhanced SOD level in mice serum and cerebrum .

结果月光花豆乙醇提取物能显著减少小鼠的扭体次数，显著延长小鼠热板反应舔足潜伏期及温浴致小鼠缩尾反应潜伏期，同时能使热板法小鼠血清和脑组织PGE2，MDA含量明显降低，SOD活性明显增强。

The models of auricular edema in mice by dimethyl benzene and increased permeability of celiac blood capillary by acetic acid were studied,the results showed that the petroleum ether and acetic ester fraction of fermentation product of Calvatia gigantea could inhibit ear edema;acetic ester and n-Butanol fraction of fermentation product could reduce the permeability of celiac blood capillary by acetic acid.

大秃马勃Calvatia gigantea发酵液的石油醚、乙酸乙酯和正丁醇提取物进行二甲苯诱导小鼠耳廓急性炎症和醋酸所致小鼠腹腔毛细血管通透性实验，结果表明大秃马勃Calvatia gigantea发酵液石油醚提取物处理组、乙酸乙酯提取物处理组对二甲苯诱导小鼠耳肿胀具有明显的抑制作用；大秃马勃Calvatia gigantea发酵液乙酸乙酯提取物处理组、正丁醇提取物处理组对醋酸所致小鼠腹腔毛细血管通透性具有明显的抑制作用。

objective to compare the anti-aging effects of lycium barbarum polysaccharides and tanshinone on the skin of d-galactose-induced mice aging model.methods 60 female mice were randomly divided into 4 groups (n=15):mice in normal control group received subcutaneous injection of normal saline and mice in the other 3 groups received cervicodorsal injection of d-galactose［1000 mg/(kg1·d)］ for 42 d to establish subacute aging model.the experimental groups included agingmodel group,lycium barbarum polysaccharides ［200 mg／group,tanshinone ［1 500 mg／］ group.forty two days later,the dorsal skin samples were collected to determine sod activities、mda and hydroxyproline contents.

目的 比较枸杞多糖与丹参酮对衰老小鼠皮肤的抗衰老作用。方法 60只雌性昆明种小鼠，随机分为4组(n=15)：正常对照组皮下注射生理盐水；其余3组每日颈背部皮下注射 d-半乳糖［1000 mg/］造成小鼠衰老模型，同时各组分别灌胃生理盐水、枸杞多糖［200 mg/］、丹参酮［1 500 mg/］。42 d后，测定小鼠背部皮肤组织匀浆中超氧化物歧化酶活力、过氧化脂质代谢产物丙二醛含量和皮肤羟脯氨酸含量。

The test of white blood cell count was finished by the different number of bifidobacterium suspension coloclysis and general irradiation with 5.0 Gy γ ray to establish acute radiation damage pattern.The 30 survival rate was tested and reported after the bifidobacterium suspension coloclysis of 1×109CFU/ml and 5.0 Gy ray to establish acute radiation damage pattern number in every day.

WBC计数：用不同数量级浓度的双歧杆菌菌悬液对小鼠灌肠28 d后，5.0 Gy照射后3 d、6 d和10 d采取小鼠外周血检测WBC数。30 d小鼠生存率：用1×109CFU/m l双歧杆菌菌悬液灌肠28 d后，用一次性8.0 Gy60Coγ射线全身照射，建立急性放射损伤模型，每日上下午各观察小鼠1次，记录死亡动物数和死亡日期。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

Results: Tyroserleutide can significantly increase the life span of H22 tumor-bearing mice by 50-70% in dosages of 20ug/kg/d-80ug/kg/d,specially the high dosage of 80ug/ml can significantly increase the life span by 69.24%; Tyroserleutide can inhibit the growth of transplanted hepatocellular tumor BEL-7402 in nude mice,the rate of tumor inhibition was25-50% in dosages of 40-320ug/ml ,the inhibition rate of 160ng/ml was 44.03%; Tyroserleutide could inhibit the growth of H22 and BEL-7402 tumor in a dose-dependent manner. Simultaneously, tumoricidal activity of tyroserleutide against BEL-7402 cell line in vitro was observed hinger when compared with the control group(P.05).The inhibition effect of 72hrs was higher than 24hrs,48hrs,96hrs.And specially the high dosage of 160ug/ml can significantly inhibit growth of tumor cell by 19.36%. Tyroserleutide can activated PEM and marked enhance cytotoxicity andphagocytosis functions in vitro and in vivo. The OD values of cytotoxicity were observed hinger when compared with the control group(P.05).The cytotoxicity of macrophages activated by tyroserleutide against BEL-7402 and B16-F10 was 35.58%,61.2% in vitro and21.39%,47.63% in vivo. The cytotoxicity rate of nude mice PEM was 32.86%,73.07% in vivo. Furthermore, tyroserleutide alone could stimulated the production of IL-1B TNF- a and NO by M . Tyroserleutide and LPS could synergistically activated M producing more cytotoxicity effectors. Conclusion: Tyroserleutide had inhibition functions against hepatoma carcinoma .Its possible mechanisms were related to the affect that Tyroserleutide could inhibit tumor cell directively and induce tumor cells apoptosis or death effectively.

结果：酪丝亮肽能显著延长腹水型肝癌H_(22)小鼠的生存时间，给药剂量为80μg／kg／d时疗效最显著，达到69.24％，在20μg／kg／d-80μg／kg／d剂量范围内生命延长率为50-70％，给药剂量与荷瘤鼠生存时间呈现一定量效关系；酪丝亮肽能显著抑制人肝癌BEL-7402移植瘤裸鼠的肿瘤生长，给药剂量为160μg／kg／d时疗效最显著，抑制率为44.03％，并且在40-320μg／kg／d剂量范围内抑制率为25-50％，给药剂量与肿瘤抑制率呈现一定量效关系；酪丝亮肽体外对人肝癌BEL-7402细胞生长有一定的抑制作用，在作用72hrs时各浓度酪丝亮肽对肿瘤细胞的抑制作用较24hrs、48hrs、96hrs明显，其中浓度为100μg／ml时抑制率达19.36％；酪丝亮肽体内外均能增强小鼠腹腔巨噬细胞对肿瘤细胞的杀伤：体外作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强，与效应细胞对照组相比有显著性差异(P＜0.05)杀伤率分别达到35.58％、61.2％；体内作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强，与生理盐水对照组相比有显著性差异(P 。05)，杀伤率分别达到21.39%、47.63%；裸鼠腹腔巨噬细胞经酪丝亮肤作用后对BEL一7402、B 16一F10杀伤功能明显增强，与生理盐水对照组相比有显著性差异(P.05)，最高杀伤率分别达到32.86%、73.07%；酪丝亮肤能增强单核巨噬细胞系统的吞噬功能，吞噬指数与生理盐水组比较有显著性差异(P.05)；酪丝亮肤体外作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO，与效应细胞对照组相比有显著性差异(P.05)；酪丝亮肤体内作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO，与生理盐水对照组相比有显著性差异(P.05)；酪丝亮肤能促进鼠巨噬细胞株R戌W264.7分泌合成IL一1p和NO，IL一1日、NO水平分别在酪丝亮肤作用24hrs、12hrs时达到高峰，酪丝亮肤单独应用能提高巨噬细胞的分泌合成功能，而且酪丝亮肤能与LPS协同作用刺激巨噬细胞的细胞毒效应分子分泌合成。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。