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General symptoms such as purpure in mucous membrance and skin, bleeding of internal organs, decreasing of weight and bad mental state caused by modeling were well improved in four control groups (Chinese traditional medicine group, western medicine group, PG original formula group and PG major formula group), the peripheral PLT was increased, spleen weight and its action of phagocytosis and damage to PLT were both decreased, morrow megakaryocytes differentiation and maturation were improved and hyperplasia descended, which showed significant differences compared with the model group. Differences among the four control groups were not significant. The optimal dose ratio of PG major formula was finded out with homogeneous design software UST3.0 and multiple regression: Astragalus root 48g, Rhubarb 15g, peach kernel 1Og, Agrimony 24g.

结果:模型建立成功;中、西药对照组、紫癜颗粒核心方组与原方组小鼠对于免疫法造模所引起的皮肤粘膜紫癜、内脏出血、体重减轻、精神状态差等一般症状均有较好的改善;小鼠的外周血小板计数得到提高;脾脏均重及对血小板的吞噬破坏降低;骨髓巨核细胞分化成熟程度增高,增生活跃度降低,与模型组相比较,均有显著性差异,四个对照组之间无显著性差异,利用均匀设计UST3.0统计软件分析,进行多元逐步回归处理,筛选出紫癜颗粒核心方的最佳剂量配比为黄芪48g,大黄15g,桃仁10g,仙鹤草24g。

An experiment was performed to compare effect of different cell media on embryonic germ cells derived from primordial germ ridge of Kunming albinotic strain mouse.

以昆明白品系小鼠胎儿生殖嵴为材料,以不同的培养液分离培养胚胎生殖细胞,发现小鼠胎儿肝细胞条件培养液的效果好于未添加白血病抑制因子的基础培养液,而差于添加了1 000 IU/mL LIF的基础培养液。

The cytotoxicity of these compounds were valuated by MTT and SRB methods using three cancer cell lines: HL-60, A-549, P-388. Cyclo-shikonin, and cyclo-alkannin have high cytotoxicity against HL-60.The chiral center of side chain has no effect on their activity. 2- 1-(Acetyloxy-4-methyl-3-pentenyl -5, 8-dihydroxy-1, 4-naphthoquinone (105) has strong inhibition effect on P-388 cells. 2- 1-(isooctyloxy-4-methyl-3-pentenyl -5, 8-dihydroxy-1, 4-naphthoquinone (108) and 2- 1-(2-furoyloxy-4-methyl-3-pentenyl -5, 8-dihydroxy-1, 4-naphthoquinone (112) have high inhibition on A-549.Shikonin (1), alkannin (2), and their derivatives have the structural features of a planar chromophores and short side chain. We examined the ability to inhibit the telomerase.

抗肿瘤活性初步筛选测试采用小鼠白血病P-388肿瘤细胞和人肺癌A-549肿瘤细胞进行,结果表明侧链羟基的手性对抗肿瘤活性无明显影响;有数个化合物具有很好的体外抑制活性,特别是化合物2-(1-异辛酰氧基-4-甲基-3-戊烯基)-5,8-二羟基-1,4-萘醌(108)和2-[1-(2-呋喃甲酰氧基)-4-甲基-3-戊烯基]-5,8-二羟基-1,4-萘醌(112)体外显示对人肺癌A-549肿瘤细胞有强效;2-(1-乙酰氧基-4-甲基-3-戊烯基)-5,8-二羟基-1,4-萘醌(105)对小鼠白血病P-388肿瘤细胞有强效;环紫草素和环异紫草素对人白血病HL-60细胞有强烈抑制作用。

Objective To explore the effects of the dose and the way and times of administration of alloxan and body weight of mice in diabetic mice model.

目的 探讨四氧嘧啶剂量、给药途径、次数和小鼠体重对四氧嘧啶致小鼠糖尿病模型的影响。

Objective: To study the effects of saponins from Anemarrhean Asphodeloides Bge on blood glucose level in normal and alloxan diabetic mice.

目的:研究知母皂苷对正常小鼠和糖尿病小鼠糖耐量及空腹血糖的影响,探讨其降糖作用机制。

Experimental observation was performed by using various models. Diabetic mice were induced by Alloxan and Adrenalin.

观察TCAE对正常小鼠血糖的影响,并分别采用肾上腺素的四氧嘧啶制备高血糖小鼠模型。

Experimental observation was perfonned by using various diabetic mice induced by alloxan, adrenalin, etc.

采用四氧嘧啶糖尿病小鼠模型以及由肾上腺素和葡萄糖引起的高血糖小鼠模型,观察TF对实验动物的降血糖效果。

Objective: To study the effects of alum, a lu minum chloride and aluminum hydroxide on aluminum contents in serum and brain of mice with high performance capillary.

中文摘要:目的:用高效毛细管电泳仪测定小鼠血清和脑组织中铝含量的方法,研究口服白矾、氢氧化铝和氯化铝对小鼠血清和脑组织中铝含量的影响。

Results It was demonstrated that β-TrCP expressed in oral epithelium, tooth bud, mesenchymal cell cytoplasm of ameloblast and edontoblast of different stage of tooth development.

结果β-TrCP在胚胎10.5、13.5、14.5、16.5、18.5d的小鼠牙胚上皮层与间充质层,以及出生后0、3、6d的小鼠成釉细胞与成牙本质细胞胞浆呈特征性表达。

In order to study the relation of cytochrome P-450 with the protective action of SY-801 and SY-640 against 〓-induced UDS and 3H-BP binding to nuclear DNA, the effects of SY-801 and SY-640 on liver microsomal cytochrome P-450 were studied in mice. The results indicated that the liver microsomal cytochrome P-450 concentration and aminopyrine demethylase activity were significantly increased in mice pretreated with SY-801 150mg/kg or SY-640 150 mg/kg once daily for three days.

本实验进而观察了SY-801对小鼠肝微粒体细胞色素P-450酶系的影响,结果表明SY-801和SY-640体内连续给药3天,可诱导小鼠肝微粒体细胞色素P-450含量明显增加,同时氨基比林脱甲基酶活性亦明显增加;给药1次2小时内二者均可抑制氨基比林脱甲基酶活性,而对肝微粒体细胞色素P-450含量则无明显影响。

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