小鼠

We spliced the pronuclei vector and microinject the remain DNA sequence to pronuclei of the mouse zygote.We got 29 mice of G0 generation ,and 8 of the 29 mice are positive result which checked by method of PCR.

构建的乳腺特异性表达载体去除原核部分(αS1-casein基因调控序列+人胰岛素原基因)，显微注射小鼠受精卵雄原核，获得29只G_0代小鼠，PCR检测出其中8只为转基因刚性小鼠。

The experimental group has various changes in the different density of potentill anserine extract. the small rat pulmonary tissue surface ofⅰgroup can see sporadic petechia of different degree; pulmonary alveolus cavity can see sporadic erythrocyte under the microscope. the small rat pulmonary tissue surface of ⅱ group is no obvious abnormality by naked eye; pulmonary alveolus cavity can see sporadic erythrocyte and thin powder color liquid under the microscope.

实验组不同浓度的蕨麻提取物对小鼠肺组织的影响不同，ⅰ组小鼠肺组织表面肉眼见不同程度的散在点状出血、显微镜下肺泡腔内见散在红细胞；ⅱ组小鼠肺组织表面肉眼无明显异常，显微镜下肺泡腔内见散在红细胞及淡伊红色液体，两组均见肺泡壁毛细血管扩张充血及毛细血管内皮细胞呈不同程度的增生肥大。

Pre-experimental results showed, sensitivity varied among mice speaces, C57BL/6J mice were more sensitive to endotoxin than Balb/c mice, Balb/c mice were more sensitive than Kunming mice.

预实验的结果显示，C57BL／6J小鼠对内毒素的敏感程度大于Balb／c小鼠，Balb／c小鼠对内毒素的敏感程度大于昆明鼠。

The mice were randomly divided into two groups ( n =24 per group): the experimental group, the cultured cells were injected into the mice through the tail vein; the control group, the equivalent serum-free medium was injected. Six mice from each group were killed at 7, 14, 21, and 28 days after operation to receive HE staining, PCR gel electrophoresis, immunohistochemistry staining, and hepatic function detection.

将小鼠随机分为实验组和对照组（ n =24），实验组将诱导后细胞（1.6 × 105 个/只，含CD34+细胞1× 104 个）通过尾静脉注射入肝损伤小鼠体内，对照组注射等量无血清培养基，术后7、14、21、28 d 两组分别处死6 只小鼠，行HE 染色、PCR 凝胶电泳、免疫组织化学染色及肝功能检测。

Results The tail formation rate of model group was significantly higher than the rate of control group (P＜0.05) with a large number of Ⅲ and Ⅳ DNA damage cells. The tail formation rate of dichloromethane extraction groups were both significantly lower than the rate of model group (P＜0.05). p53 mRNA expression of model group was higher than the rest except the crude Cornus Officinalis group.

结果 D-半乳糖致衰模型组小鼠骨髓细胞DNA拖尾率高于正常对照组(P＜0.05)，且Ⅲ、Ⅳ级损伤细胞数较多；山茱萸生品及制品二氯甲烷萃取部位组小鼠骨髓细胞DNA的拖尾率均低于D-半乳糖致衰模型组(P＜0.05)。D-半乳糖致衰模型组小鼠骨髓细胞p53基因的表达高于正常对照组、阳性药组及制品组。

Method］ Observe its influence on the pain tolerance of mouse hot plate method, on mouse ear swelling caused by xylene, on the cut-tail blood time of mouse, and on rats' hemoglutination platelet collection.

方法］观察对小鼠热板法痛阈值的影响；对二甲苯所致小鼠耳廓肿胀的影响；对小鼠断尾出血时间的影响；对大鼠凝血机能及血小板凝集性的影响。

The control group were injected peritoneal physiological saline injection.Three study groups were injected acrylamide solution 5 mg.kg-1.d-1,30 mg.kg-1.d-1,and 60 mg.kg-1.d-1,respectively.On the 5th day,brains of each group were taken out quickly,fixed by Zamboni fixation solution,made into conventional freezing moviemaking,stained by immunohistochemistry staining,and then observed by light microscope.

对照组小鼠腹腔注射生理盐水；实验组小鼠分别代以5 mg·kg-1·d-1、30 mg·kg-1·d-1、60 mg·kg-1·d-1丙烯酰胺水溶液腹腔注射。5 d时分别处死各组小鼠，取出大脑，Zamboni固定液固定，常规冰冻制片，免疫组织化学染色，光镜观察。

Glucose load can significantly lower blood glucose in mice, but also significantly against adrenaline-induced increase in blood glucose response in mice, and it can obviously confrontation Phenformin mice caused by hypoglycemia.

可使葡萄糖负荷的小鼠血糖明显降低，也能明显对抗肾上腺素引起的小鼠血糖升高反应，而且它还能明显对抗苯乙双胍引起的小鼠实验性低血糖。

Antiviral 〔HBV, HCV and HIV〕, and anti-inflammatory 〔of Shigella dysenteriae, Bacillus anthracis, a, b-type melting Streptococcus pneumoniae, Staphylococcus aureus, E. coli, salmonella have both antibacterial antibacterial activity〕,〔Jiangtang glucose load can significantly lower blood glucose in mice, but also significantly against adrenaline-induced increase in blood glucose response in mice, and it can obviously confrontation Phenformin mice caused by hypoglycemia .

抗病毒〔 HBV ， HCV 与 HIV 〕，，抗炎〔对志贺氏痢疾杆菌，炭疽杆菌， a 、 b 型溶链球菌肺炎双球菌，金黄色球菌，大肠杆菌、沙门氏菌均有抗菌均有抗菌作用〕，降糖〔可使葡萄糖负荷的小鼠血糖明显降低，也能明显对抗肾上腺素引起的小鼠血糖升高反应，而且它还能明显对抗苯乙双胍引起的小鼠实验性低血糖。

PTH gene and protein expression levels were up-regulated in parathyroid gland and thymus in 1αase knock-out mice with hypocalcaemia.

1αase-/-小鼠表现为低钙血症？与正常血钙的WT小鼠相比，PTH基因和蛋白的表达水平在1αase-/-小鼠胸腺组织中均明显升高？

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。