小鼠

Methods: Kuming mice has been employed in this experiment, which divied into several groups. the blood lactate, blood urea nitrogen and glycogen were detected after loading swimming .

方法和结果：本课题通过对实验组、对照组的雄性昆明种小鼠负重游泳后的血乳酸水平的差异与变化；实验组、对照组的雄性昆明种小鼠游泳后血清尿素氮的差异与变化；实验组、对照组的雄性昆明种小鼠肝糖原含量的差异与变化，来判定受试物是否具有抗疲劳的作用。

METHODSMice with swelling of the earflaps induced by xylene, proliferation of granulation tissue induced by cotton pellets were treated with ethanol extract of the walnut leaf, and the weight of immune organ was weighed.

方法］采用二甲苯致小鼠耳壳肿胀，植入棉球致小鼠肉芽组织增生及称取免疫器官质量等方法，观察核桃叶乙醇提取物对小鼠抗炎作用及对免疫器官质量的影响。

Methods The immunofluorescency, Western blotting and RT-PCR were performed on mouse liver tissue obtained from 4 experimens pcDNA3.1(+-HBX and 1 normal control, 24 h after delivery the HBX gene eukaryon expression vector pcDNA3.1-HBX into mouse by the means of hydrodynamic injection.

实验动物分成模型组和对照组，模型组利用已构建的含有HBX基因的真核表达质粒pcDNA3.1-HBX，对照组以等量生理盐水代替，采用流体动力学法将质粒经尾静脉高压注入小鼠体内，24h后取小鼠肝组织，行免疫荧光、RT-PCR和Western blot法从不同水平检测HBX在小鼠肝组织内的表达情况。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

Results The expression of endogenous LN was found in the basement membrane of epithelium, glands and blood vessels in uterine endometrium of both estrus and early pregnancy. And its was faint that LN present in basement membrane as the implantation occurs, but the LN immunostaining in the epithelial cells of endometrium was stronger. The exogenous LN has no obvious influence on blastocyst attachment and implantation. LN-antibody intra-uterine injection showed significant repressing effect on mouse blastocyst implantation.

结果 动情期及妊娠早期小鼠子宫内膜内源性LN在上皮和腺基膜及血管内皮基膜均有表达，并随着植入的发生基膜的LN表达减弱，而内膜上皮中LN免疫染色逐渐增强；外源性LN对小鼠胚泡粘附与植入子宫内膜不产生明显影响，层粘连蛋白抗体明显抑制小鼠胚泡着床。

The result showed HSV seropositive mice was really induced to produce tumor special or non-special factors, possibly included natural killer cell and lymphokine activated killer cell, which cooperated with mtHSV to kill tumor cells in course of mtHSV mediated tumor therapy. The effect in wtHSV seropositive mice was better than in mtHSV seropositive mice.

实验证明HSV免疫小鼠确实诱导产生了肿瘤特异性或非特异性因子，可能包括NK细胞和LAK细胞(lymphokine activated killer cell)，这些肿瘤特异性或非特异性因子在mtHSV介导的肿瘤治疗中与mtHSV一起协同杀死了肿瘤细胞，HSV-1F免疫小鼠的这种作用强于mtHSV免疫小鼠。

The sixth, there were no significantly differences between colchicine groupsand vinblastine groups on the metaphases, in the experiment of prepare mouse4-cell embryo stage single blastomere chromosome specimen by using optimumparameters; and there were significantly differences between colchicine groupsand vinblastine groups on the metaphases (P＜0.05), in the experiment of preparemouse 8-cell embryo stage single blastomere chromosome specimen by usingoptimum parameters.The end, the preparation quality of mouse 4-and 8-cell embryo stage singleblastomere specimen were 2.5, 2.7, 2.4, 1.9, respectively.

低渗液选用0.45％氯化钠时，低渗效果优于选用0.5％柠檬酸钠和0.35％氯化钾的。6、在利用最佳参数制备小鼠4-细胞期胚胎单卵裂球染色体标本时，经统计分析，两种阻断剂的作用效果差异不显著；而在制备小鼠8-细胞期胚胎单卵裂球染色体时阻断剂选用长春花碱的效果优于秋水仙素的(P＜0.05)。7、秋水仙素组和长春花碱组小鼠4、8-细胞胚胎单卵裂球染色体标本制备质量分别为：2.5、2.7、2.4、1.9。

Methods: nutmeg volatile oil gavage route of administration used to observe and calculate the value of LD50 in mice was observed in mice dose of pentobarbital sodium Excellency the hypnotic synergy strychnine convulsion convulsion caused by mice and the percentage of the incubation period, The incubation period the percentage of death and survival, as well as on coronary artery ligation in rats with acute myocardial infarction confrontation role.

肉豆蔻挥发油采用灌胃给药途径，观察并计算小鼠LD50值，观察对小鼠阁下剂量戊巴比妥钠的催眠协同作用，对士的宁引起小鼠惊厥的惊厥潜伏期及百分率、死亡潜伏期及存活百分率的影响，以及对冠脉结扎所致大鼠急性心肌梗死的对抗作用。

Tpap is an age-dependent gene in mouse testis. The expression of Tpap corresponds to the appearance of spermatids of mice and indicates that Tpap may have an important role in male mammalian spermatogenesis.

结果表明，Tpap基因为小鼠年龄依赖性表达基因，小鼠Tpap的表达与小鼠精子发生的过程有很强的一致性，推测该基因在哺乳动物精子发生中起重要作用。

In 3 of 14 mice surviving long-term transplanted with bone marrow cells transduced with high-titer virus, bone marrow, spleen and thymus from two mice and bone marrow and spleen from another mouse contained the intact proviral genome. Long-term expression of the transferred gene was seen in one mouse at level of 7%of endogenous murine α-globin gene.

5%。在2只长期造血重建小鼠的骨髓、脾、胸腺及另一只小鼠的骨髓及脾中整合有人β-珠蛋白基因，在一只重建小鼠的骨髓和脾中有人β-珠蛋白基因表达，约为鼠α-珠蛋白基因的7 ％。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。