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It is estimated that there are about 40,000 functional genes.In order to understand those genes related to human diseases and the novel functional genes, the genetic manipulated mice including transgenic mice and knockout mice have been generated to facilitate the purpose.This transgenic core has been collaborated with industrial and research institutes for years, and has built up competitive know-how for transgenic mice, embryo cryopreservation, and pathogen free rederivation.

小鼠长期以来即为研究人类疾病最佳动物模式,2001年美国赛洛因公司已将小鼠129的两个品系之基因序列解码完成提供付费使用,2003年小鼠B6的基因序列也被Mouse Genomic DNA Sequencing Consortium解码完成,预估约有4万个功能基因,随著基因操控技术的成熟,制造出各种带不同基因缺陷的模式动物供生物医学研究已非难事。

The results of the research are suggested that it is successful to construst the transgenic mice pBC-rtTA which is a regulated element of tetracycline system and a tool mouse for our lab and also successful to construst the transgenic mice which are conditional expression of Simian Virus 40 T Antigen leading to regulatable development of tumor by tetracycline system.

试验结果证明本实验成功建立转基因小鼠pBC-rtTA,该小鼠可作为实验室的转基因工具小鼠,是四环素调控系统中的调控元件。

RESULTS:Naokangtai capsule improved the abilites of active and passive learning and memory remarkedly in the model mice with cerebral ischemia-reperfusion injury,but there was no influence to the contents of MDA,SOD and MAO.In senile dementia mice induced by D-galactose,Naokangtai capsule possessed obvious effects of improving memory and spatial resolution and decreased contents of MDA and MAO in blood significantly.

结果:脑康泰胶囊显著增强脑缺血再灌注损伤模型小鼠被动和主动学习能力,但对血液MDA、MAO和SOD含量影响不显著;脑康泰除明显增强老年痴呆小鼠主动学习和空间分辨的能力外,还能显著降低小鼠血液中MDA和MAO水平。

Results:All dosage groups of Tou-Yu-Tong Pills could release chronic nerve injury caused by formaldehyde solution; remarkedly inhibit the pain of mice in the formalin test and prolong the latencies of paw-licking. It could significantly diminish pain caused by glacial acetic acid ,reduce the number of the writhing in mice.To the model of migraine ,Tou-Yu-Tong Pills can improve the content of 5-HT in brain tissue of mice ,and ruduce 5-HTAA.

结果 头愈痛丸对福尔马林致小鼠慢性神经性疼痛反应,能明显减少动物舔后足的时间,改善其伤害性疼痛行为反应;对冰醋酸致小鼠扭体反应,能显著延长扭体的潜伏期,减少扭体反应的次数;对小鼠偏头痛模型,能显著升高小鼠脑内5-HT的含量,且降低5-HIAA的趋势。

To establish the replantable models of tumon cell line S 180 and EAC using BALB/c mice respectively.

应用小鼠S1 80 肉瘤,小鼠EAC腹水瘤实体型两种瘤株,进行BALB c小鼠移植瘤模型的研究。

And 1‰ test groups were fed with 0.4‰, 0.8‰, 1‰ formaldehyde sulphoxylate solution. In the 10 and 30 day, the mice were killed, the cerebrum tissuce were took out, and fixed in the Zamboni fixation solution, conventionally produced frozen section and stained by immunohistochemistry.

对照组小鼠喂食静置自来水;实验组小鼠分别代以0.4‰、0.8‰、1‰次硫酸氢钠甲醛溶液喂食,10d和30d时分别处死各组小鼠10只,取出大脑,Zamboni固定液固定,常规冰冻制片,免疫组织化学染色,光镜观察。

Methods:Human CD80 cDNA was transfected into B16 melanoma cells by lipofectin-mediated gene transfer before the expansion of tumor cells were tested by MTT method in vitro;C57BL/6 mice were inculated subcutaneously either mock-transfected B16 cells (B16-neo)or CD80-transfected B16 cells (B16-CD80),then the latency,survival times and tumor mass growth were investigated.The lymphocytes were examined for both proliferation indices and specific cytotoxic activity by MTT method and improved LDH-releasing method,after syngenic Mixed Lymphocyte tumor cultures.

通过脂质体介导将CD80基因导入小鼠黑色素瘤B16细胞后,利用SABC法检测CD80的表达;MTT法测定肿瘤细胞体外增殖能力;将肿瘤细胞接种至同源小鼠皮下后观察成瘤期、荷瘤小鼠存活期及肿瘤结节生长速度;在同源淋巴细胞肿瘤细胞混合培养后,通过测定淋巴细胞增殖指数和CTL杀伤活性检测肿瘤细胞的免疫原性。

Anti-filament immunofluorescence indicated the distribution of primary vestibular neuron endings in vestibular sensory epithelium and the contacts between it and hair cells were normal.These results indicated that Smad4 conditional knockout mice were generated successfully, whose morphological and pathological changes in vestibulum auris internae were slightly.

该研究提示,软骨组织Smad4条件基因敲除小鼠动物模型造模成功;Smad4条件基因敲除小鼠的内耳前庭形态轮廓基本正常但细节上有一定程度的畸形,纯合子小鼠前庭末梢感受器病理改变轻微。

Results: Ghrelin ameliorated significantly the clinical and histopathologic seerity of the trinitrobenzene sulfonic acid-induced colitis; abrogating body weight loss, diarrhea, and inflammation; and increasing surial.

结果:Ghrelin能够明显改善三硝基苯磺酸诱导的小鼠结肠炎的临床和组织病理学改变;能够消除小鼠的体重减轻、腹泻和炎症;并且能增加小鼠生存率。

NOD/SCID (non-obese diabetic/severe combined immunodeficient) mice are immunodeficient mice which are generated by backcrossing severe combined immunodeficient mice with non-obese diabetic mice strains.

NOD/SCID(非肥胖糖尿病/重症联合免疫缺陷)小鼠是在SCID小鼠的基础上与非肥胖性糖尿病小鼠品系回交的免疫缺陷鼠。

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