小鼠

In order to examine the safety of Evening Primrose Oil Capsule in toxicology, a series of tests are performed, including the acute peroral toxicity test in mice, Ames test, PCE micronucleus test in Bone Marrow cells on mice, sperm abnormality test on mice and 30 days feeding test on rat.

为了探讨其毒理学安全性，本研究利用小鼠急性经口毒性试验、鼠伤寒沙门氏菌/哺乳动物微粒体酶试验、小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸形试验、30天喂养试验等方法对月见草油胶囊进行了食品安全毒理学检测。

Methods Mammary adenocarcinoma EMT6 was cultured and propagated, then implanted on female BALB/c mice. Tea polyphenols was peroral administrated and local injected, C-Jun in tumor tissue was detected by immunohistochemical method.

应用小鼠可移植性乳腺癌EMT6细胞系，经培养传代后，以纯系BALB/c小鼠为荷瘤动物进行肿瘤移植，并采用茶多酚灌胃及局部注射两种干预措施，以免疫组化方法检测小鼠乳腺癌组织C-Jun表达。

Monocytes generated from bone marrow of Balb/cj mouse were cultured for 6 days in complete RPMI 1640 medium containing 10％ FBS, rmGM-CSF and rmIL-4.50 mg·L-1 acteoside or isoacteoside was added to cells on day 6 of culture for 24 h.The surface molecules expression level of DCs and their phagocytose ability were analysis by flow cytometry.

采用细胞因子诱导法，从Balb/cj小鼠骨髓细胞贴壁分离获得单核细胞，加入含10%胎牛血清、10 μg·L-1重组小鼠粒细胞巨噬细胞集落刺激因子及重组小鼠白细胞介素-4(rmIL-4)的RPMI 1640完全培养基培养，在培养第6天，实验组加入车前子苯乙醇苷类化合物(10，50，100 mg·L-1)，对照组加入RPMI 1640或LPS(1 mg·L-1)，流式细胞仪检测DCs表面分子CD11c，CD86，MHC II和CD80的表达以及各组DCs吞噬功能的变化。

Many tumor specific antigens such as mouse PlA, human MAGE, BAGE and GAGE families had been isolated during recent decade. PlA is a tumor specific antigen expressed by mouse mastocytoma P815. It is one kind of cancer-testis antigens and expresses in various mouse tumor such as mastocytoma P815, plasmoma J558 and fibrosarcoma Meth A but hardly in normal tissue except testis and placenta.

近十年来已经成功分离出多种鼠或人的肿瘤特异性抗原，如小鼠P1A基因和人的MAGE、BAGE、GAGE家族等。P1A为小鼠肥大细胞瘤P815所表达的肿瘤特异性抗原，它是一种CT抗原，在多种小鼠肿瘤如肥大细胞瘤P815、浆细胞瘤J558和纤维肉瘤Meth A等中表达，而在除睾丸、胎盘之外的正常组织中不表达。

The mutation potential of ethephon was by investigated the micronucleus test of bone marrow polychromatophilic erythroblastsPCE cells and sperm shape abnormality test in mice.

为测定乙烯利对人体健康的影响，采用小鼠骨髓细胞微核试验和小鼠精子畸形试验分别检测了乙烯利对小鼠体细胞和生殖细胞的致突变性。

The results confirmed that inhibition of JNK indeed improve disorder in morphology, pathology and praxiology in MPTP PD model. Our research offers a new strategy for researching anti-PD drugs.

JNK抑制剂也改善了PD小鼠的行为学，使小鼠在爬杆试验、悬挂试验和游泳试验中的评分显著提高，提示小鼠的肢体协调能力有所改善。

In order to test the probability of Math1 gene induces hair cell regeneration in cochlea of presbycusis mice,we prosecuted the study and divided into three parts as following.

本研究拟行C57BL/6小鼠老年性聋动物模型评价；小鼠耳蜗外源基因导入途径及方法的研究；老年性聋C57BL/6小鼠内耳Math1基因导入后毛细胞样细胞的观察。

Intracytoplasmic sperm injection is a very important assistant reproductive technology for treatment of man infertility and fundamental research , Success in ICSI in human and other animals has recently been achieved., The present pilot studies were conducted to establish a preliminary procedure for ICSI in mice, explore the experimental conditions of ICSI in mice, investigate factors influencing the test results, and accumulate technologic basis for the next studies on pronuclear exchange in mice .

本部分研究论文初步建立了小鼠ICSI技术的基本操作程序，摸索了小鼠ICSI研究的操作条件，总结了一些影响显微注射效果的重要因素，并为下一部分小鼠原核置换的研究奠定了雄厚的技术基础。

Objective To investigate adenoviral vector mediated exogenous gene expression in mouse lun gs and the effect of mIFN-γ transgene expression on allergen-induced pulmonar y eosinophil infiltration in a murine asthmatic model.

目的研究重组复制缺陷腺病毒在小鼠肺内气道上皮细胞中的转基因表达水平及其介导小鼠γ-干扰素的转基因表达对过敏原诱导的小鼠哮喘模型的预防性治疗作用。

We found that both SL7207 and SL7207/pEGFP-N1 vaccines can not induce theprotective immunity,while SL7207/pGFP-FL vaccines can trigger antitumorresponses in partial (2/10) of orally immunized mice.And these two tumor-free micealso got the immunity to rechallenge the 10-fold dose of H1 tumor cells when given40 days post the primary challenge.

我们发现，野生型的SL7207和SL7207/pEGFP-N1均不能诱发小鼠的保护性免疫，而SL7207/pGFP-FL则可以在部分小鼠（2/10）中诱发保护性免疫，使小鼠免生肿瘤，并能抵抗在初次荷瘤攻击后40天，10倍于首次剂量的H1肿瘤细胞的再攻击。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐