小鼠

The mouse was carried out half nephric duct ligation to induce the renal interstitial fibrosis animal model,afterwards,to set up three groups,control group,sham operation group(intragastric administration with normal sodium according to 10ml/kg weight),treatment group...

采用小鼠单侧输尿管结扎法诱导肾间质纤维化的动物模型，用盐酸青藤碱治疗，并设对照组、假手术组(小鼠每日按10m l/kg体重用生理盐水灌胃，直至治疗结束)、治疗组(选用正清风痛宁缓释片按每日30mg/kg体重给小鼠灌胃，连续3周，)，检测血尿素氮、血肌酐、血清层粘连蛋白、纤维连接蛋白的含量，免疫组化法测肾脏组织转化因子β1(TNF-β1)的表达并进行肾脏病理学的观察。

Methods The effects of Wuweizi Ningshen Oral Liquid on sedation,hypnotics and anti-convulsion in mice were observed by counting spontaneous behavior,prolonging sleeping time cooperated with pentobarbital sodium and antagonizing convulsion induced by Nikethamide injection.

采用镇静、催眠、抗惊厥实验观察五味子宁神口服液对小鼠自主活动的影响，协同戊巴比妥钠对小鼠的催眠作用及对小鼠尼可刹米惊厥的抑制作用。

MethodsThe effects of Wuweizi Ningshen Oral Liquid on sedation,hypnotics and anti-convulsion in mice were observed by counting spontaneous behavior,prolonging sleeping time cooperated with pentobarbital sodium and antagonizing convulsion induced by Nikethamide injection.

方法采用镇静、催眠、抗惊厥实验观察五味子宁神口服液对小鼠自主活动的影响，协同戊巴比妥钠对小鼠的催眠作用及对小鼠尼可刹米惊厥的抑制作用。

MAIN OUTCOME MEASURES: Morphological changes in primary cultured and subcultured mouse BMSCs were measured. BMSC surface antigen of the second passage of mice (CD105, CD44, CD25, CD34) were determined using flow cytometry. The modified method was assessed to harvest the purity of stem cells. Activity of mouse BMSCs was identified using adipogenic and osteoplastic differentiation. The strength of fluorescent cells following multiple passage was observed.

主要观察指标：原代及传代小鼠骨髓间充质干细胞形态变化；对第2代小鼠骨髓间充质干细胞表面抗原，CD105，CD44，CD25，CD34进行流式细胞仪检测，评价改良方法获取干细胞纯度；通过成脂成骨分化，鉴定小鼠骨髓间充质干细胞的活性；观察多次传代后荧光细胞强度。

Results The results indicated that SIPAR was expressed in otic vesicles and eye vesicles obviously in mouse dpc9.5 embryo.

结果 SIPAR在dpc9.5的小鼠胚胎眼泡和听泡中有明显的表达，在dpc9.5～12.5的小鼠胚胎脊柱神经和脑部中枢神经都有表达，在dpc12.5小鼠胚胎脑、眼泡和脊柱中表达增强。

These ES cells were introduced into host embryos from outbred KMW though blastocyst injection. Using GFP, the attractive genetic marker, we have been able to follow the fate of ES cells in living embryos and to observe directly the contribution of these cells to mouse embryos. Totally, four GFP-expressing 'green' chimeric mice expressing GFP were obtained.

为研究嵌合体动物中供体胚胎干细胞在宿主胚胎发育中的走向和定位，同时探讨绿色荧光蛋白基因作为报告基因在转基因动物制作中的应用价值，本研究将pEGFP-N1基因导入小鼠ES-D3细胞系，得到稳定表达GFP的胚胎干细胞亚系ES-D3-GFP，通过对昆明小鼠的囊胚腔注射，获得了4只表达绿色荧光蛋白的嵌合体小鼠。

Methods Colony-forming unit of spleen, number of nucleated cells in bone marrow and spleen index were used to investigate the effect of polysaccharides at 50, 80 mg/kg from parmelia tinctorium intraperitoneal injection on hematopoietic function of mice irradiated with 137Cs γ-rays; DNA double-strand breaks were detected using alkaline single cell gel electrophoresis.

用内源性脾结节形成，骨髓有核细胞计数，脾脏指数观察石花多糖50，80 mg/kg腹腔注射对137 Csγ射线照射小鼠造血功能的影响；单细胞凝胶电泳技术检测小鼠淋巴细胞照射后的DNA双链断裂，观察给药后小鼠DNA损伤修复情况。

The microcirculation disturbance in mice was brought by rapid injection of adrenaline.The micro vascular diameter,the blood flow speed and micrangium crossing net patefaction number in auricle of mice at normal condition and in the 10,20,30 min after injection of adrenaline were observed by microcirculation microscope with analytical system.

连续给药4 d，末次给药30 min时将小鼠固定于微循环观测分析系统显微镜下，观测小鼠正常及腹腔注射盐酸肾上腺素(1 mg·kg-1)10、20和30 min时小鼠耳廓微血管的管径、血流速度及毛细血管交叉网开放数目。

In the in vivo study, 100 ICR mice were randomly assigned to 5 groups after paired by body weight as following: Group N (negative control group, peritoneally injected by saline daily); Group C (positive control group, peritoneally injected by D-galactose and saline daily); Group L, M, and H (peritoneally injected by D-galactose and intragastric administered with TP at 100, 200, 400 mg/day, respectively). Mice were sacrificed for histology and biochemistry studies after 8 weeks.

在动物体内实验中，选择清洁级ICR纯系小鼠100只，按体质量随机分为5组：阴性对照组每天腹腔注射生理盐水，其余4组每天腹腔注射120 mg/kg D-半乳糖以建立小鼠衰老模型，同时阳性对照组每日以生理盐水灌胃，L、M、H组分别以每日100、200、400 mg/kg TP灌胃。8周后处死小鼠，获取心肌标本进行组织学研究和生化检测。

Effect of A_3 on dimethylbenzene-induced mice ear edema Method:After peroral A_3 3 days, the mice ear edema models induced by dimethylbenzene were prepared. The mice ear edema was tested at 1 h.

A_3对二甲苯所致小鼠耳廓肿胀的影响方法：连续灌胃给药3天(1次/天)后，采用二甲苯制备小鼠耳廓肿胀的炎症模型，观察二甲苯致炎后1 h药物对小鼠耳肿胀的影响。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐