小鼠

In mice, Zanthoxylum bungeanum and lower dosage (500,1000mg/kg) of Illicium verum , Cinnamomum japonicum do not damage the bone marrow cell chromosome. But in high dosage, the Zb can inhibit the bone marrow cellproliferation, and the Iv and Cj can at different degree influence the stability of bone marrow cell chromosome.

花椒组和低剂量的八角、桂皮对小鼠骨髓细胞染色体没有损伤作用，高剂量花椒对小鼠骨髓细胞的增殖有一定抑制作用，高剂量八角、桂皮对小鼠骨髓细胞染色体的稳定性有不同程度的影响。

Animal models were worked out by injecting granule solution into the lung of mice through trachea to study pulmonary lesions in mice. All mice were intratracheally instilled every three days. At the tenth day, mice were sacrificed and. Lung organ coefficient, albumin, total protein, acid phosphatase and Alkaline phosphatase were measured.

为研究纳米二氧化钛对小鼠肺部的损伤，将小鼠采用非暴露式气管内注入法注入颗粒悬液建立动物模型，每隔2d染毒一次，在第10d处死小鼠，测定肺脏器系数及肺部组织中白蛋白、总蛋白、酸性磷酸酶和碱性磷酸酶的含量。

It shows that Iridoids composition of Valeriana jatamansi Jones have inhibit function on gastrointestinal function of normal mice.1.2 Effection of Iridoids composition of Valeriana jatamansi Jones on gastrointestinal function of mice with reserpineMethods:Compared with normal group,model group and YJBH group,mice with reserpine which has given Iridoids composition of Valeriana jatamansi Jones as 71.2mg/kg,35.6mg/kg,17.8mg/kg,8.9mg/kg was detected gastric emptying and small intestinal propulsion after 6 days drug given.

结果：蜘蛛香环烯醚萜35.6mg/kg、17.8mg/kg组能减缓胃排空和小肠推作用(P.05)，说明蜘蛛香环烯醚萜对正常小鼠胃肠功能有一定的抑制作用。1.2蜘蛛香环烯醚萜对利血平化小鼠胃肠功能的作用方法：小鼠每日腹腔注射利血平造模，同时灌胃给予蜘蛛香环烯醚萜71.2mg/kg、35.6mg/kg、17.8mg/kg、8.9mg/kg与空白组、模型组和越鞠保和丸组对照，给药6日后测定动物的胃排空率和小肠推进率。

Considering the merits of in vivo studies in biotransformation i. e. activation or inactivation, protein-binding such as sex hormone binding protein , toxicokinetics, and more evaluative in hazard identification and risk assessment, we selected a battery of short-term and long-term in vivo studies, including uterotrophic assay in weaning mice and ovariectomized adult mice, an in vivo multiple endpoints assay, and a gestational and lactational exposure assay to identify the estrogenic effects of endosulfan on the offspring.

考虑到体内试验在代谢转化、血浆蛋白如性激素结合蛋白的结合、代谢动力学等方面比体外试验优越，在危害鉴定和危险度评定中更有价值，我们选择了多项体内试验，包括对幼年小鼠和卵巢切除成年小鼠的子宫增殖作用、对成年去势小鼠的体内多终点观察以及大鼠孕期和哺乳期接触对雄性子代生殖系统结构和功能的改变，即去雄性化或雌性化作用等，来验证硫丹的体内雌激素样作用。

In result 2.5% of the rats got slight hepatic enzymes increase, and 6.25% of the rats got slight fat degeneration for hepatic tissue specimens. Most of the rats with abmormal results occurred in the toxic and lethiferous group, and there was the increasing tendency with the increase of dose. There were no changes for renal function and tissue biopsy.

实验结果显示共有2.5％的小鼠出现了轻度的肝酶升高，组织病理有6.25％的小鼠肝组织出现了轻度的脂肪变性，结果异常的小鼠绝大多数发生于中毒剂量及致死剂量组，且有随着剂量增高而增加的趋势；肾功能及组织病理无明显改变。

The result fights allergic experiment to show, dispel the wind stops land of can different level restrains urticant Ding allergy of akin and passive skin mixes the bandicoot that antiserum causes 2, the sex of small rat late hair of chloric benzene be caused by exceeds 2 nitro- of 4- quick the thymus index that reaction; still can increase small rat when dosage greatly and lienal index; stop urticant experiment shows, dispel the wind stops urticant Ding can reduce the time of fit of small rat itching with dextrose lead anhydride and itching duration apparently, can increase Guinea pig to be able to bear or endure those who suffer phosphoric acid histamine send urticant threshold.

结果抗过敏试验显示，祛风止痒酊能不同程度地抑制抗血清诱发的大鼠同种被动皮肤过敏和2，4-二硝基氯苯所致的小鼠迟发性超敏反应；大剂量时还可增加小鼠的胸腺指数及脾指数；止痒试验显示，祛风止痒酊能明显降低右旋糖酐诱导的小鼠搔痒发作次数及搔痒持续时间，并能增加豚鼠耐受磷酸组胺的致痒阈。

Results: In acute experiment, there was no significant difference of body weight or organ indexes between the normal control and arsenic poisoning groups ; while the CH50 level, lymphocyte accruing rate, phagocytosis rate and macrophagocyte index in arsenic poisoning group were significantly lower (P.05 or P.01) than those in normal group. There was no significant difference of body weight or organ index between arsenic poisoning group and EXD (10 g) treated arsenic poisoning group, while CH50, lymphocyte accruing rate, phagocytosis rate, and mavrophocyte index were significantly higher in EXD (10 g) treated arsenic poisoning group than those in arsenic poisoning group (P.05 or P.01). In EXD (5 g) treated arsenic poisoning group, results of all the tested indexes were the same as those of arsenic poisoning group.

结果：与正常对照组相比，砷中毒组小鼠体重、脏器指数无明显改变；CH50、淋巴细胞增殖、巨噬细胞吞噬率及吞噬指数明显降低，差异有显著性（P.05或P.01；与砷中毒组相比，竹荪治疗低剂量组小鼠体重、脏器指数、CH50、淋巴细胞增殖、巨噬细胞吞噬率及吞噬指数无明显改变化；竹荪治疗高剂量组除小鼠体重、脏器指数无明显改变外，CH50、淋巴细胞增殖、巨噬细胞吞噬率及吞噬指数明显升高，差异有显著性（P.05或P.01）。

Micrococcal lysis assay of the milk samples showed that the cDNA was efficiently expressed in the mammary glands with similar expression levels ranging from 60 to 87 mg/L. Different tissues of each vector-injected mouse were assayed for transcription of hLYZ mRNA by dot-blotting and for expression of hLYZ activity by Micrococcal lysis assay. The results showed that expression of the three recombinant vectors was mainly restricted to mammary glands with some degrees of ectopic expression in spleen, intestines and/or kidney.

注射后72 h采集乳样，经微球菌溶解实验证明，3组小鼠乳汁中hLYZ的表达量分别为87、69、60 mg/L；从每组小鼠的12种组织提取总RNA，经Dot blotting检测证明，三种重组载体除在乳腺中表达外，p205C3LYZ还在小鼠的脾和肠、pBJLYZ在脾、pBCLYZ在脾和肾中具有一定的异位表达；在有hLYZ mRNA转录的上述脏器中用微球菌溶解试验均可检测到hLYZ活性。

Methods:Prepared multilaminar liposome vaccine by film evaporaqtion combined with lyophilization; Immunized BALB/c mice with the obtained liposomeencapsulated influenza split vaccine and the routine influenza split vaccine, serum antibodies were assayed on weeks 213 by the haemagglutinationinhibition test and ELISA, the HI test for the special antiHA antigen and the ELISA test for the IgG. Protective immunity against intranasal virus challenge was determined at 12 weeks postvaccination.

采用薄膜法与冷冻干燥法相结合的方法制得多层脂质体；用制得的脂质体流感疫苗和普通疫苗分别免疫小鼠，用血凝抑制实验和酶联免疫吸附试验对免后2～13周的小鼠血清的抗体水平进行检测，HI检测其特异性抗体，ELISA检测IgG抗体水平并对小鼠的保护效率进行检测。

The results show that Yiliqi can enhance the delayed type hepersensitivity of mouse and phagocytosis of macrophage,and it can also increase the positive rate of peripheral T cell containing acid α-naphthyl acetate esterase and the number of antibody forming cell in spleen.

实验结果表明，小鼠连续20天给与该受试物，在人使用量的1倍、10倍、30倍剂量组能明显增强小鼠迟发型变态反应及增加外周血T淋巴细胞ANAE阳性率，并可提高小鼠巨噬细胞吞噬功能和脾脏抗体生成细胞数。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐