小鼠

In vivo, composition E markedly protected mice from lethal challenge by heat-killed E. coli and heat-killed S. aureus. Conclusions:(1) Targeting on PGN, CpG DNA and lipid A, we successfully established the platform to screen anti-inflammatory traditional Chinese medicine using biosensor technology which is highly effective, objective and feasible.

该组分经MTT法测定对小鼠RAW264.7细胞活力无影响(≤400μg/ml)，经CCK-8法测定也对小鼠RAW264.7细胞活力无影响(≤800μg/ml)；对PGN、CpG DNA及LPS刺激小鼠RAW264.7细胞分泌TNF-α有显著的抑制作用，并呈现良好的剂量-效应关系；对致死剂量的热灭活大肠杆菌和热灭活金黄色葡萄球菌攻击小鼠有显著的保护作用。

Methods ICR Mice of tumor model was made by inoculated with tumor cells (S180, EAC, and Heps). The antitumor activity of brucine and its liposome was evaluated by tumor inhibitory ratio for S180 and Heps and life prolonging rate for EAC and Heps of mice with transplanted tumor.

用ICR小鼠接种S180、EAC腹水瘤、肝Heps瘤株造成相应的移植性荷瘤小鼠模型，考察马钱子碱及其脂质体对实体瘤模型（S180和Heps）小鼠的抑瘤率和对腹水瘤模型小鼠生命延长率的作用以研究两者的抗肿瘤活性。

METHOD: ICR male mice of tumor model was made by inoculating with tumor cells (S180, EAC, Heps). Anti-tumor activity of brucine was evaluated by tumor inhibitory ratio and life prolonging rate of mice transplanted with tumor. RESULT: Brucine had significant restraining effect on transplanted tumor growth of mice.

用ICR小鼠接种S180、EAC、Heps瘤株造成相应的移植性荷瘤小鼠模型，考察马钱子碱对实体瘤模型(S180、Heps)小鼠的抑瘤率和对腹水瘤模型小鼠生命延长率以研究其抗肿瘤活性。

Results Brucine and its liposome had significant inhibiting effect on transplanted tumor growth in mice. Inhibitory rates of brucine on Heps were 35.05% [1.61mg/], 43.70% [3.23mg/], and 46.09% [6.46mg/], while of its liposome on Reps was 45.41% 1.61mg/1 and 58.19% [3.23 mg/, respectively. The inhibitory rate of brucine on S180 was 37.59% [1.61 mg/], 36.13% 3.23mg/, and 38.87% [6.46 mg/, while of its liposome on S180 was 53.00% [1.61mg/] and 48.37% [3.23mg/], respectively. However, both of them had no distinct effect on prolonging the life of mice with transplanted tumor.

结果 马钱子碱及其脂质体能有效抑制实体瘤模型荷瘤小鼠体内肿瘤生长，1.61、3.23、6.46mg/马钱子碱对Heps小鼠的抑瘤率分别为35.05%、43.70%和46.09%，而1.61、3.23mg/马钱子碱脂质体的抑瘤率分别为45.41%和58.19%；1.61%、3.23、6.46mg/马钱子碱对S180小鼠的抑瘤率分别为37.59%、36.13%和35.57%，而1.61和3.23mg/马钱子碱脂质体的抑瘤率分别为53.00%和48.37%，明显高于马钱子碱；但两者对腹水瘤模型荷瘤小鼠的生存时间均无延长作用。

Moreover, in order to make clear whether IV infection touches off the cause and its pathogenesis of artherosclerosis inflammatory reaction. The study also uses IV of type A1 and A3 to infect repeatedly Jimpy mice with E gene defect, to observe AS histology change of Jimpy mice aorta and detect the change of IV antibody and inflammatory reaction medium (eg.TNF-α, IL-1) in Jimpy mice serum and express of monocyte chemotactic factor( MCF-1) and nuclear factor kB in Jimpy mice aorta tissue .

另外，为了弄清流感病毒感染是否是触发动脉粥样硬化炎症反应的发生原因及其机理，本研究还用A1、A3型流感病毒反复感染载脂蛋白E基因缺陷小鼠，观察小鼠主动脉的动脉粥样硬化组织学改变，并检测小鼠血清中流感病毒抗体、炎症反应介质（如TNF-a、IL-1等）变化情况以及小鼠主动脉组织中的单核细胞趋化因子-1（MCP-1）和核转录因子kB表达情况。

Methods Tumor-bearing mouse models were set up by transplanting hepatoma (H22), sarcoma S180 and leukemia (L1210). Tumor inhibitory action and life-prolonging effect of AIDI on the mice, as well as its synergism with CTX were observed.

抗肿瘤体内实验：建立小鼠移植性肉瘤S180和小鼠肝癌H22的实体瘤模型，艾迪粉针剂对小鼠尾静脉给药，连续处理7d，第8天剥离瘤块称质量，计算抑瘤率，与化疗药合并用药考察减毒增效作用；建立小鼠L1210白血病模型，阳性对照组为顺铂，考察生命延长率。

Results and ConclusionThe study of time-efficacy relation expresses that after infusing the solution of Phyuanthus emblica Phyllanthus emblica, medicated serum in different time activates the spleen lymphocyte of small mice differently after the 30min but before 180min. During this period, on 2h the activation of the medicated serum is the most obvious. The quantity-efficacy relation research expresses that megadose promotes the propagate of the spleen lymphocyte of small mouse and activates the macrophage in the abdominal cavity more obviously. On the other hand, the small dosage influents the survival rate of the mouse S180 ascites tumor cell more obviously.

结果与结论时效关系研究表明，用余甘子冻干粉溶液灌胃后30～180 min内不同时相含药血清对小鼠脾淋巴细胞具有活化作用，并在给药2 h时实验血清活化作用明显；量效关系研究表明，余甘子大剂量组实验血清对促进小鼠脾淋巴细胞增殖，促进小鼠腹腔巨噬细胞细胞活化作用以及小剂量组实验血清对小鼠S180腹水肿瘤细胞存活率的影响均具显著差异性。

Adoping the simple and easy anthology to gather the unit to gather urine of 5 hours measures, Watch different series of urine quantitative changeization .(2) Experiment of immunity: Adopted CY methods copy immunity to repress the little rat pattern, To divide randomly small healthy white mice originated from kunming into normal contrast group ,pattern group, Fulin group, Guizhi group,Fulin and Guizhi group (Two medicine are according to 1 : 1、2: 1、 1: 2、 4: 3 the proportion compose ).Be total to 8 groups . To observed Fulin and Guizhi different combinition adjust the repressing small mice's spleen and thymus, Ascertaining by measuring little rat phagocyte the gobbleing uprate and serum IL-2 -* TNF — a 's content.

2免疫实验采用环磷酰胺法复制免疫抑制小鼠模型，健康昆明种小鼠随机分为正常对组、模型组、茯苓组、桂枝组、茯苓与桂枝配伍组(两药按1：1、2：1、1：2、4：3比例进行组合)共8组，观察茯苓桂枝药对及其各配伍组对免疫抑制小鼠脾、胸腺的影响，测定小鼠吞噬细胞吞噬率及血清IL-2、TNF—α含量。

Methods 16 female mice with alcoholic liver fibrosis were randomly divided into group A and B. The mice in group A(n=8) received syngeneic male bone marrow CD117+Lin- cell transplantation. The mice in group B(n=8) received isovolumetric Buffer 2 solutions infusion.

16只酒精性肝纤维化雌性小鼠随机分为移植组和对照组，移植组小鼠（n=8）接受同基因型雄性小鼠的骨髓CD117+Lin-细胞移植，对照组小鼠（n=8）接受等体积的Buffer2液输注。

Methods 16 female mice with carbon tetrachloride liver fibrosis were randomly divided into group A and B. The mice in group A (n=8) received syngeneic male bone marrow C-kit+Lin-cell transplantation. The animals in group B (n=8) received isovolumetric Buffer2 solutions infusion.

16只四氯化碳性肝纤维化雌性小鼠随机分为移植组和对照组，移植组小鼠（n=8）接受同基因型雄性小鼠的骨髓C-kit+Lin-细胞移植，对照组小鼠（n=8）接受等体积的Buffer2液输注。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。